SrtA (sortase)

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Plasmid
pET28a-S-tag-SrtA 60-206
Parent Plasmid
pET28a original
Expressed
6His-S-tag-SrtA 60-206
DU Number
DU20983
Genbank
ADC38675
Tag
6His
Species
S. aureus
Sequence of Insert

CATATGAAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGAC
AGCCAAGCTAAACCTCAAATTCCGAAAGATAAATCAAAAGTGGCAGGCTA
TATTGAAATTCCAGATGCTGATATTAAAGAACCAGTATATCCAGGACCAG
CAACACCTGAACAATTAAATAGAGGTGTAAGCTTTGCAGAAGAAAATGAA
TCACTAGATGATCAAAATATTTCAATTGCAGGACACACTTTCATTGACCG
TCCGAACTATCAATTTACAAATCTTAAAGCAGCCAAAAAAGGTAGTATGG
TGTACTTTAAAGTTGGTAATGAAACACGTAAGTATAAAATGACAAGTATA
AGAGATGTTAAGCCAACAGATGTAGAAGTTCTAGATGAACAAAAAGGTAA
AGATAAACAATTAACATTAATTACTTGTGATGATTACAATGAAAAGACAG
GCGTTTGGGAAAAACGTAAAATCTTTGTAGCTACAGAAGTCAAATAAGGA
TCC

Amino Acid Sequence
MGSSHHHHHHSSGLVPRGSHMKETAAAKFERQHMDSQAKPQIPKDKSKVA
GYIEIPDADIKEPVYPGPATPEQLNRGVSFAEENESLDDQNISIAGHTFI
DRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRDVKPTDVEVLDEQK
GKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK*
Vector Type
Bacterial
Antibiotic
Kan
Comments
Bacterial expression plasmid for 6HIS- S-tag-SrtA aa 60-206

Estimated MW of expressed protein : 20694 Daltons
100042 R 1000 1001 1047 19007
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