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Synonyms
CUL4B
Plasmid
pSC-B-Cullin4b
Parent Plasmid
pSC-B
DU Number
DU20034
Genbank
NM_003588
Species
Human
Sequence of Insert
View Sequence
GGATCCATGATGTCACAGTCATCTGGATCAGGAGATGGGAATGATGATGAGGCTACTACCTCTAAAGACGGTGGTTTTTCTTCCCCCAGTCCCTCAGCTGCTGCTGCTGCTCAGGAGGTCAGATCTGCCACTGATGGTAATACCAGCACCACTCCGCCCACCTCTGCCAAGAAGAGAAAGTTAAACAGCAGCAGCAGTAGCAGCAGTAACAGTAGTAACGAGAGAGAAGACTTTGATTCCACCTCTTCCTCCTCTTCCACTCCTCCTTTACAACCCAGGGATTCGGCATCCCCTTCAACCTCGTCC
TTCTGCCTGGGGGTTTCAGTGGCTGCTTCCAGCCACGTACCGATACAGAA
GAAGCTGCGTTTTGAAGACACCCTGGAGTTTGTAGGGTTTGATGCGAAGA
TGGCTGAGGAATCCTCCTCCTCCTCCTCCTCATCTTCACCAACTGCTGCA
ACATCTCAGCAGCAGCAACTTAAAAATAAGAGTATATTAATCTCTTCTGT
GGCTTCGGTGCATCATGCAAACGGCCTAGCCAAATCTTCTACCACCGTCT
CTAGCTTTGCTAACAGCAAACCTGGCTCTGCTAAGAAGTTAGTGATCAAG
AACTTTAAAGATAAGCCTAAATTACCAGAAAACTACACAGATGAAACCTG
GCAAAAACTGAAAGAAGCAGTGGAAGCTATTCAGAATAGTACTTCAATTA
AGTACAATTTAGAAGAACTCTACCAGGCTGTAGAAAATCTCTGTTCTTAC
AAGATTTCTGCAAACTTGTACAAACAGCTGAGACAGATCTGCGAAGATCA
CATCAAAGCACAGATTCATCAATTCAGAGAGGATTCATTGGATAGCGTTC
TTTTTTTAAAGAAGATTGATAGATGCTGGCAAAACCATTGCAGACAAATG
ATCATGATCAGGAGCATTTTTTTGTTTCTGGATAGAACTTACGTTCTTCA
GAATTCAATGCTACCCTCCATTTGGGACATGGGACTGGAGTTATTTAGGG
CTCATATTATAAGTGATCAGAAAGTGCAGAATAAGACAATTGATGGCATT
CTTCTCTTGATTGAGAGGGAAAGGAATGGTGAAGCAATTGATAGAAGTTT
ACTTCGAAGCCTTTTAAGCATGCTGTCTGATTTGCAAATTTATCAAGATT
CTTTTGAACAACGATTTTTGGAAGAAACTAACCGGCTCTATGCAGCTGAA
GGCCAAAAATTAATGCAAGAAAGAGAGGTTCCTGAATATCTACATCATGT
TAACAAACGTCTAGAAGAAGAAGCAGACAGACTTATTACTTACTTAGATC
AGACCACCCAGAAGTCATTAATTGCTACTGTAGAAAAACAACTTCTAGGT
GAACACTTAACAGCAATTCTTCAGAAAGGTTTAAATAACCTCCTTGATGA
AAACCGAATTCAAGATTTGTCTCTTCTGTATCAGCTCTTCAGTAGAGTTC
GAGGTGGAGTTCAGGTTCTTTTGCAGCAGTGGATCGAATATATCAAGGCA
TTTGGCAGCACTATTGTAATTAATCCTGAAAAAGATAAAACCATGGTTCA
AGAATTGCTGGATTTTAAAGATAAGGTTGACCATATAATTGATATCTGCT
TTCTGAAGAATGAGAAATTTATCAATGCCATGAAAGAAGCATTTGAAACG
TTCATTAACAAAAGACCAAATAAACCAGCTGAACTTATAGCTAAGTATGT
AGATTCAAAACTTCGTGCAGGCAACAAAGAAGCTACAGATGAAGAACTTG
AGAAAATGTTGGATAAAATTATGATCATATTTAGATTTATCTATGGCAAG
GATGTTTTTGAGGCCTTCTATAAGAAAGATTTAGCCAAGCGCCTGTTAGT
CGGAAAGAGTGCATCTGTAGATGCTGAAAAATCAATGCTGTCCAAACTTA
AACATGAATGCGGAGCTGCTTTCACCAGCAAACTTGAAGGAATGTTTAAA
GACATGGAACTTTCTAAAGACATCATGATTCAGTTCAAACAGTATATGCA
GAATCAGAATGTTCCGGGAAATATTGAGTTAACTGTGAATATCCTGACAA
TGGGCTATTGGCCGACATATGTGCCTATGGAAGTTCATTTACCACCAGAG
ATGGTAAAACTTCAGGAGATTTTCAAGACATTTTACCTAGGCAAACATAG
TGGCAGGAAACTTCAGTGGCAGTCAACCCTAGGACACTGTGTGTTAAAAG
CAGAATTTAAAGAGGGTAAAAAGGAACTCCAGGTCTCTCTTTTTCAAACA
CTGGTGCTGCTAATGTTTAATGAGGGAGAGGAGTTCAGTTTAGAAGAGAT
CAAGCAGGCAACTGGAATAGAGGATGGAGAGTTAAGGAGAACACTGCAGT
CATTAGCCTGTGGCAAAGCTAGAGTTCTGGCGAAAAATCCAAAGGGCAAA
GACATTGAAGATGGTGACAAGTTCATTTGTAATGATGATTTCAAACATAA
ACTTTTCAGGATAAAGATCAATCAAATCCAGATGAAAGAAACGGTTGAAG
AACAAGCAAGCACTACAGAAAGAGTATTTCAAGACAGACAGTATCAAATT
GATGCTGCAATTGTTCGAATTATGAAGATGAGAAAGACACTTAGCCACAA
TCTCCTTGTTTCAGAAGTGTACAACCAGTTGAAATTTCCAGTAAAGCCTG
CTGATCTTAAGAAGAGAATAGAATCTTTAATTGACCGGGACTACATGGAA
AGAGATAAAGAAAATCCAAACCAGTACAACTATATTGCATAGGCGGCCGC
TTCTGCCTGGGGGTTTCAGTGGCTGCTTCCAGCCACGTACCGATACAGAA
GAAGCTGCGTTTTGAAGACACCCTGGAGTTTGTAGGGTTTGATGCGAAGA
TGGCTGAGGAATCCTCCTCCTCCTCCTCCTCATCTTCACCAACTGCTGCA
ACATCTCAGCAGCAGCAACTTAAAAATAAGAGTATATTAATCTCTTCTGT
GGCTTCGGTGCATCATGCAAACGGCCTAGCCAAATCTTCTACCACCGTCT
CTAGCTTTGCTAACAGCAAACCTGGCTCTGCTAAGAAGTTAGTGATCAAG
AACTTTAAAGATAAGCCTAAATTACCAGAAAACTACACAGATGAAACCTG
GCAAAAACTGAAAGAAGCAGTGGAAGCTATTCAGAATAGTACTTCAATTA
AGTACAATTTAGAAGAACTCTACCAGGCTGTAGAAAATCTCTGTTCTTAC
AAGATTTCTGCAAACTTGTACAAACAGCTGAGACAGATCTGCGAAGATCA
CATCAAAGCACAGATTCATCAATTCAGAGAGGATTCATTGGATAGCGTTC
TTTTTTTAAAGAAGATTGATAGATGCTGGCAAAACCATTGCAGACAAATG
ATCATGATCAGGAGCATTTTTTTGTTTCTGGATAGAACTTACGTTCTTCA
GAATTCAATGCTACCCTCCATTTGGGACATGGGACTGGAGTTATTTAGGG
CTCATATTATAAGTGATCAGAAAGTGCAGAATAAGACAATTGATGGCATT
CTTCTCTTGATTGAGAGGGAAAGGAATGGTGAAGCAATTGATAGAAGTTT
ACTTCGAAGCCTTTTAAGCATGCTGTCTGATTTGCAAATTTATCAAGATT
CTTTTGAACAACGATTTTTGGAAGAAACTAACCGGCTCTATGCAGCTGAA
GGCCAAAAATTAATGCAAGAAAGAGAGGTTCCTGAATATCTACATCATGT
TAACAAACGTCTAGAAGAAGAAGCAGACAGACTTATTACTTACTTAGATC
AGACCACCCAGAAGTCATTAATTGCTACTGTAGAAAAACAACTTCTAGGT
GAACACTTAACAGCAATTCTTCAGAAAGGTTTAAATAACCTCCTTGATGA
AAACCGAATTCAAGATTTGTCTCTTCTGTATCAGCTCTTCAGTAGAGTTC
GAGGTGGAGTTCAGGTTCTTTTGCAGCAGTGGATCGAATATATCAAGGCA
TTTGGCAGCACTATTGTAATTAATCCTGAAAAAGATAAAACCATGGTTCA
AGAATTGCTGGATTTTAAAGATAAGGTTGACCATATAATTGATATCTGCT
TTCTGAAGAATGAGAAATTTATCAATGCCATGAAAGAAGCATTTGAAACG
TTCATTAACAAAAGACCAAATAAACCAGCTGAACTTATAGCTAAGTATGT
AGATTCAAAACTTCGTGCAGGCAACAAAGAAGCTACAGATGAAGAACTTG
AGAAAATGTTGGATAAAATTATGATCATATTTAGATTTATCTATGGCAAG
GATGTTTTTGAGGCCTTCTATAAGAAAGATTTAGCCAAGCGCCTGTTAGT
CGGAAAGAGTGCATCTGTAGATGCTGAAAAATCAATGCTGTCCAAACTTA
AACATGAATGCGGAGCTGCTTTCACCAGCAAACTTGAAGGAATGTTTAAA
GACATGGAACTTTCTAAAGACATCATGATTCAGTTCAAACAGTATATGCA
GAATCAGAATGTTCCGGGAAATATTGAGTTAACTGTGAATATCCTGACAA
TGGGCTATTGGCCGACATATGTGCCTATGGAAGTTCATTTACCACCAGAG
ATGGTAAAACTTCAGGAGATTTTCAAGACATTTTACCTAGGCAAACATAG
TGGCAGGAAACTTCAGTGGCAGTCAACCCTAGGACACTGTGTGTTAAAAG
CAGAATTTAAAGAGGGTAAAAAGGAACTCCAGGTCTCTCTTTTTCAAACA
CTGGTGCTGCTAATGTTTAATGAGGGAGAGGAGTTCAGTTTAGAAGAGAT
CAAGCAGGCAACTGGAATAGAGGATGGAGAGTTAAGGAGAACACTGCAGT
CATTAGCCTGTGGCAAAGCTAGAGTTCTGGCGAAAAATCCAAAGGGCAAA
GACATTGAAGATGGTGACAAGTTCATTTGTAATGATGATTTCAAACATAA
ACTTTTCAGGATAAAGATCAATCAAATCCAGATGAAAGAAACGGTTGAAG
AACAAGCAAGCACTACAGAAAGAGTATTTCAAGACAGACAGTATCAAATT
GATGCTGCAATTGTTCGAATTATGAAGATGAGAAAGACACTTAGCCACAA
TCTCCTTGTTTCAGAAGTGTACAACCAGTTGAAATTTCCAGTAAAGCCTG
CTGATCTTAAGAAGAGAATAGAATCTTTAATTGACCGGGACTACATGGAA
AGAGATAAAGAAAATCCAAACCAGTACAACTATATTGCATAGGCGGCCGC
Vector Type
Other
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
IMAGE clone source carries a c2179a mutation conferring Leu727Ile. This was corrected in this clone using mutagenic oligos 55184/55185.