ZNRF2 (genomic)

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Plasmid
pAAV + SEPT + ZNRF2 targeting construct
Parent Plasmid
pAAV MCS
DU Number
DU40015
Genbank
NM_147128.3 GI:40316919
Species
Human
Sequence of Insert
gcggccgcCATTTTTATATGTGCAATTTAATAGCATTTAAGACATTCACAACCCCCATCTTTATCTGGTTCTAAACACTTCATCGCCCCAAAAGAAAACCTCTGGTGTGATTCCACTTACATGAGGTAGCTAAAATACTCAAACTCATAGAAGCAGAGAGTAGACTAGTGGTGGCCAGGGGCTGGGCGGGGGGAAGGGGAGTGGTGGATTAATGGGTATAACATTTTAGTTATGCACGATGAGTAAGTTCTAGACATCTGCCATACAACATAGTGCCTACAGTTGACAATACTATaTTGTACACTTGAAGATTTGTTAAGAGGATAGAGCATATGTTAGTCTTACCACAATAAAATAAAGTTTTTAAAAGGGGAAAAAACGGGTCAGGCGTGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGTGGATCACGAGGTCAGGAGTTCGAGACCAGCCCGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAACTAGCCAGGCGTGGTGGTGGGCGCCTGTAATCCTAGCTACTCGGGAGGCTGAGACAGGAAAATCACTTGAACCCAGGAGGCGGGGGTTGCAGTGAGCCGAGATAGTGCCACTGCACTCCAGTCTGGGTGACAGAGTGAGACTCCATCTCAAATAAAAGCGGGGTGGTGGGGGGGGAGAAAACCCCCTACCCATTAAGCAGTCAtTCCCCATATCCCTCTGTCCCCAATACTGATCTCCTTTCCCTCTCTATGGATGTATCTATTCTGGACATTTCATATAAATGGGTTCATACAATAGGTGGTTTCTTTTACTTTGTGTAGTATTTTTGACGTTTATCCATGTTTTAGCATGTGGTGGTTCTTCCTTTTCTTGGCTTAGTAATATTCCATTGTGTGGATACATCATCTTTTGTTTATCCGTTGCGCAGTTTGTAGACACTGGGTTGTTTCCACCTTTGGGCTGTTGTGAACAATGCTGCTGTGAACCTCCGTGCACAAGGATTTTCTTGGGGACCTGTTTCTTCCTCTCATTCTTGCCTCCTACCACTCTACCCATTACCATATTTTTCCAAACCAGACATCAGCACACTGGCTACGTGTGTGTTTAGGAAAGCATCTGTGGAGGGAAACAGCTTGCCTCATGCTGCCCCTGCTTCCTGTCTACCCTTCTCCAAGCCTTTTTCGTCCGCTGTCACCgagctccaccgcggtggcggccgctctagaactagtggatccataacttcgtatagcatacattatacgaagttatggaattaattcgctgtctgcgagggccagctgttggggtgagtactccctctcaaaagcgggcatgacttctgcgctaagattgtcagtttccaaaaacgaggaggatttgatattcacctggcccgcggtgatgcctttgagggtggccgcgtccatctggtcagaaaagacaatctttttgttgtcaagcttgaggtgtggcaggcttgagatctggccatacacttgagtgacaatgacatccactttgcctttctctccacaggtgtccactcccaggtccaactgcaggtcgagcgtcgagaacgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgttattttccaccatattgccgtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtctttcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctctggaagcttcttgaagacaaacaacgtctgtagcgaccctttgcaggcagcggaaccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacacctgcaaaggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatggctctcctcaagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtatgggatctgatctggggcctcggtgcacatgctttacatgtgtttagtcgaggttaaaaaaacgtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgatgataagcttgccacaaccatggctgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgggggatccagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttctagggggccccggcgaggccgggatgagttgggaataacttcgtatagcatacattatacgaagttatccaaattttaaggtaccactgtgcatatgtatgaattcGGGAGCTGATGGGGAGACCCGTAGAGCCTCACAGGTTGGAGGAGCTTCGGATCCCACTGGGAAGGTTGGTTCTCCGTCTGTCTCCCTGCCTCTTCTTCCTCTACGGGTCCCTCTGCTCCACAGGGGTAGAACATCAATCTGTGCGAGGAAGGCCAGGCGGAGGGTGTACCCACTGCCTTGCACTGGCCTTCTCCCTAGAGGGCCGGGAGGCAGGAAGAGCCATTTCCTGTGGGGCCACAGCACTGGGCACAGTTAAAAGTAGCAGGGCCCAGATATGCCTTGGGACTCCAGTGTGAGCCTCGTCCTTGTTTCCAGCTGGAAGGAAGGCACCCTCTTGCCCAAGACAGGACACTGTGCTGCCTGGGGCCAGCACCTGcTGAATCCTCGAGCCCAGAGCTTCTTCCTGACACTCAGGCCTTTCAAAGCCTGACTCTCAGGGCCACTGCTGCACAGGGTGACAACCGGGCATGGCACGCAGTGTGGCTGGTGCAGAGGAGGCTTAAGGCTGGCCTGGGAGAGATTCTTGGTCTAGCTCCCAGCACGGAGCCTTGCTTGTGTCCCAGGGCGGCACTAACCAGAACCAGAAAAGGAAATCTTTGGCCCTCGGGGAGAATTATGCCAATTATCTGTCTGGTTCCTGTCTTTGAAAATGCCCAGATGTTGGTTTACAAATCAGGAAGCTGTCTTGGGGCAATCTCTGTTCCCTCTTTGATGCAGAGAGCCTTTCTCTGTAAACACTGGAGAGCAGGGCTTCACTGTCTGGCTCCCCTCCCGCCTCTTGCAGGAAGGACTAGATGCTGCTGATCAGCTGATCATGGCTGAGCCGGGTAGAGCTTGGGGCTCTCCTAAGAGGTCACCCACTGGGAGGGAGCTTCACAGCTGTTCTTTTGACCCAGGAAGGAGCTCTTTCAGGACTGTGGCACCCAGAGCCGTGGCCCTCCTGCCATGCCATTGTTCCCACTTCCCTGCCCTTGGGAGTCTTGGAGACAGCTGACTAGCTGCCCTGAGGGGTCCACCGCTCTCACCTTCTCACTGGTGGCTGCTGCCATTCTCCTGGGCTCTGCACAGAAAGCTTTGGTCCTTCTGAAAGCCACTCTGCCCCTTCCTGCAGCACCAGATCCCCCTGAGCCGGTACTGCTGCCTCCACCTGGGCCAAGACTAGTCgcggccgc
Vector Type
Other
Antibiotic
Amp
Comments
For knock-out of ZNRF2 using Bunz method.

All ZNRF2 plasmids MUST be grown at 30 degrees C or less to prevent recombination.
100042 R 1000 1001 1047 19007
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