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UBC1

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Plasmid
pET156P-UBC1
Parent Plasmid
pET15b 6His 3C
Expressed
6His-UBC1
DU Number
DU20018
Genbank
NM_005339
Tag
6His
Species
Human
Sequence of Insert
GGATCCATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGA
GGTGCTGAAGAGCGAGGAGACGAGCAAAAATCAAATTAAAGTAGATCTTG
TAGATGAGAATTTTACAGAATTAAGAGGAGAAATAGCAGGACCTCCAGAC
ACACCATATGAAGGAGGAAGATACCAACTAGAGATAAAAATACCAGAAAC
ATACCCATTTAATCCCCCTAAGGTCCGGTTTATCACTAAAATATGGCATC
CTAATATTAGTTCCGTCACAGGGGCTATTTGTTTGGATATCCTGAAAGAT
CAATGGGCAGCTGCAATGACTCTCCGCACGGTATTATTGTCATTGCAAGC
ACTATTGGCAGCTGCAGAGCCAGATGATCCACAGGATGCTGTAGTAGCAA
ATCAGTACAAACAAAATCCCGAAATGTTCAAACAGACAGCTCGACTTTGG
GCACATGTGTATGCTGGAGCACCAGTTTCTAGTCCAGAATACACCAAAAA
AATAGAAAACCTATGTGCTATGGGCTTTGATAGGAATGCAGTAATAGTGG
CCTTGTCTTCAAAATCATGGGATGTAGAGACTGCAACAGAATTGCTTCTG
AGTAACTGAGCGGCCGC
Amino Acid Sequence
MGSSHHHHHHSSGLEVLFQGPGSMANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN*


Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial expression plasmid to express Ubc1 (E2-25K) with N-terminal 6HIS tag (contains preScission protease site)

Estimated MW of expressed protein : 24851 Daltons
100042 R 1000 1001 1047 19007
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