UBE2E3 | Ubiquitylation Resource

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Plasmid

pET28a(+) UBE2E3

Parent Plasmid

pET28a original

Expressed

6H-UBE2E3

DU Number

DU14049

Genbank

NM_006357

Tag

6His

Species

Human

Sequence of Insert

View Sequence

AGATCTATGTCCAGTGATAGGCAAAGGTCCGATGATGAGAGCCCCAGCAC
CAGCAGTGGCAGTTCAGATGCGGACCAGCGAGACCCAGCCGCTCCAGAGC
CTGAAGAACAAGAGGAAAGAAAACCTTCTGCCACCCAGCAGAAGAAAAAC
ACCAAACTCTCTAGCAAAACCACTGCTAAGTTATCCACTAGTGCTAAAAG
AATTCAGAAGGAGCTAGCTGAAATAACCCTTGATCCTCCTCCTAATTGCA
GTGCTGGGCCTAAAGGAGATAACATTTATGAATGGAGATCAACTATACTT
GGTCCACCGGGTTCTGTATATGAAGGTGGTGTGTTTTTTCTGGATATCAC
ATTTTCATCAGATTATCCATTTAAGCCACCAAAGGTTACTTTCCGCACCA
GAATCTATCACTGCAACATCAACAGTCAGGGAGTCATCTGTCTGGACATC
CTTAAAGACAACTGGAGTCCCGCTTTGACTATTTCAAAGGTTTTGCTGTC
TATTTGTTCCCTTTTGACAGACTGCAACCCTGCGGATCCTCTGGTTGGAA
GCATAGCCACTCAGTATTTGACCAACAGAGCAGAACACGACAGGATAGCC
AGACAGTGGACCAAGAGATACGCAACATAAGCGGCCGC

Amino Acid Sequence

View Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRRSMSSDRQRSDDESPSTS
SGSSDADQRDPAAPEPEEQEERKPSATQQKKNTKLSSKTTAKLSTSAKRI
QKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITF
SSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSI
CSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYAT*

Vector Type

Bacterial

Antibiotic

Kan

Comments

N ter 6HIS for bacterial expression.

Estimated MW of expressed protein : 26554 Daltons

100042 R 1000 1001 1047 19007

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