UBC1 | Ubiquitylation Resource

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Plasmid

pMalP UBC1

Parent Plasmid

pMAL6P

Expressed

MBP-UBC1

DU Number

DU20008

Genbank

NM_005339

Tag

MBP

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGA
GGTGCTGAAGAGCGAGGAGACGAGCAAAAATCAAATTAAAGTAGATCTTG
TAGATGAGAATTTTACAGAATTAAGAGGAGAAATAGCAGGACCTCCAGAC
ACACCATATGAAGGAGGAAGATACCAACTAGAGATAAAAATACCAGAAAC
ATACCCATTTAATCCCCCTAAGGTCCGGTTTATCACTAAAATATGGCATC
CTAATATTAGTTCCGTCACAGGGGCTATTTGTTTGGATATCCTGAAAGAT
CAATGGGCAGCTGCAATGACTCTCCGCACGGTATTATTGTCATTGCAAGC
ACTATTGGCAGCTGCAGAGCCAGATGATCCACAGGATGCTGTAGTAGCAA
ATCAGTACAAACAAAATCCCGAAATGTTCAAACAGACAGCTCGACTTTGG
GCACATGTGTATGCTGGAGCACCAGTTTCTAGTCCAGAATACACCAAAAA
AATAGAAAACCTATGTGCTATGGGCTTTGATAGGAATGCAGTAATAGTGG
CCTTGTCTTCAAAATCATGGGATGTAGAGACTGCAACAGAATTGCTTCTG
AGTAACTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSMANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN

Vector Type

Bacterial

Antibiotic

Amp

Comments

Bacterial expression plasmid to express Ubc1 (E2-25K) with N-terminal MBP tag (contains preScission protease site)

Estimated MW of expressed protein : 69148 Daltons

100042 R 1000 1001 1047 19007

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