UBE2F | Ubiquitylation Resource

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Plasmid

pGEX6P-1-UBE2F

Parent Plasmid

pGEX6P1

Expressed

GST-UBE2F

DU Number

DU14051

Genbank

NM_080678

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGCTAACGCTAGCAAGTAAACTGAAGCGTGACGATGGTCTCAA
AGGGTCCCGGACGGCAGCCACAGCGTCCGACTCGACTCGGAGGGTTTCTG
TGAGAGACAAATTGCTTGTTAAAGAGGTTGCAGAACTTGAAGCTAATTTA
CCTTGTACATGTAAAGTGCATTTTCCTGATCCAAACAAGCTTCATTGTTT
TCAGCTAACAGTAACCCCAGATGAGGGTTACTACCAGGGTGGAAAATTTC
AGTTTGAAACTGAAGTTCCCGATGCGTACAACATGGTGCCTCCCAAAGTG
AAATGCCTGACCAAGATCTGGCACCCCAACATCACAGAGACAGGGGAAAT
ATGTCTGAGTTTATTGAGAGAACATTCAATTGATGGCACTGGCTGGGCTC
CCACAAGAACATTAAAGGATGTCGTTTGGGGATTAAACTCTTTGTTTACT
GATCTTTTGAATTTTGATGATCCACTGAATATTGAAGCTGCAGAACATCA
TTTGCGGGACAAGGAGGACTTCCGGAATAAAGTGGATGACTACATCAAAC
GTTATGCCAGATGAGCGGCCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMLTLASKLKRDDGLKGSRT
AATASDSTRRVSVRDKLLVKEVAELEANLPCTCKVHFPDPNKLHCFQLTV
TPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLTKIWHPNITETGEICLSL
LREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIEAAEHHLRDK
EDFRNKVDDYIKRYAR*

Vector Type

Bacterial

Antibiotic

Amp

Comments

N ter GST for bacterial expression.

Estimated MW of expressed protein : 47900 Daltons

100042 R 1000 1001 1047 19007

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