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Plasmid
pET28a(+) UBE2F
Parent Plasmid
pET28a original
Expressed
6H-UBE2F
DU Number
DU14052
Genbank
NM_080678
Tag
6His
Species
Human
Sequence of Insert
View Sequence
GGATCCATGCTAACGCTAGCAAGTAAACTGAAGCGTGACGATGGTCTCAA
AGGGTCCCGGACGGCAGCCACAGCGTCCGACTCGACTCGGAGGGTTTCTG
TGAGAGACAAATTGCTTGTTAAAGAGGTTGCAGAACTTGAAGCTAATTTA
CCTTGTACATGTAAAGTGCATTTTCCTGATCCAAACAAGCTTCATTGTTT
TCAGCTAACAGTAACCCCAGATGAGGGTTACTACCAGGGTGGAAAATTTC
AGTTTGAAACTGAAGTTCCCGATGCGTACAACATGGTGCCTCCCAAAGTG
AAATGCCTGACCAAGATCTGGCACCCCAACATCACAGAGACAGGGGAAAT
ATGTCTGAGTTTATTGAGAGAACATTCAATTGATGGCACTGGCTGGGCTC
CCACAAGAACATTAAAGGATGTCGTTTGGGGATTAAACTCTTTGTTTACT
GATCTTTTGAATTTTGATGATCCACTGAATATTGAAGCTGCAGAACATCA
TTTGCGGGACAAGGAGGACTTCCGGAATAAAGTGGATGACTACATCAAAC
GTTATGCCAGATGAGCGGCCGC
AGGGTCCCGGACGGCAGCCACAGCGTCCGACTCGACTCGGAGGGTTTCTG
TGAGAGACAAATTGCTTGTTAAAGAGGTTGCAGAACTTGAAGCTAATTTA
CCTTGTACATGTAAAGTGCATTTTCCTGATCCAAACAAGCTTCATTGTTT
TCAGCTAACAGTAACCCCAGATGAGGGTTACTACCAGGGTGGAAAATTTC
AGTTTGAAACTGAAGTTCCCGATGCGTACAACATGGTGCCTCCCAAAGTG
AAATGCCTGACCAAGATCTGGCACCCCAACATCACAGAGACAGGGGAAAT
ATGTCTGAGTTTATTGAGAGAACATTCAATTGATGGCACTGGCTGGGCTC
CCACAAGAACATTAAAGGATGTCGTTTGGGGATTAAACTCTTTGTTTACT
GATCTTTTGAATTTTGATGATCCACTGAATATTGAAGCTGCAGAACATCA
TTTGCGGGACAAGGAGGACTTCCGGAATAAAGTGGATGACTACATCAAAC
GTTATGCCAGATGAGCGGCCGC
Amino Acid Sequence
View Sequence
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMLTLASKLKRDDGLKG
SRTAATASDSTRRVSVRDKLLVKEVAELEANLPCTCKVHFPDPNKLHCFQ
LTVTPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLTKIWHPNITETGEIC
LSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIEAAEHHL
RDKEDFRNKVDDYIKRYAR*
SRTAATASDSTRRVSVRDKLLVKEVAELEANLPCTCKVHFPDPNKLHCFQ
LTVTPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLTKIWHPNITETGEIC
LSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIEAAEHHL
RDKEDFRNKVDDYIKRYAR*
Vector Type
Bacterial
Antibiotic
Kan
100042 R 1000 1001 1047 19007
Download datasheet
Estimated MW of expressed protein : 24620 Daltons