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UBC1

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Plasmid
pGEX6P-UBC1
Parent Plasmid
pGEX6P1
Expressed
GST-UBC1
DU Number
DU20013
Genbank
NM_005339
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGA
GGTGCTGAAGAGCGAGGAGACGAGCAAAAATCAAATTAAAGTAGATCTTG
TAGATGAGAATTTTACAGAATTAAGAGGAGAAATAGCAGGACCTCCAGAC
ACACCATATGAAGGAGGAAGATACCAACTAGAGATAAAAATACCAGAAAC
ATACCCATTTAATCCCCCTAAGGTCCGGTTTATCACTAAAATATGGCATC
CTAATATTAGTTCCGTCACAGGGGCTATTTGTTTGGATATCCTGAAAGAT
CAATGGGCAGCTGCAATGACTCTCCGCACGGTATTATTGTCATTGCAAGC
ACTATTGGCAGCTGCAGAGCCAGATGATCCACAGGATGCTGTAGTAGCAA
ATCAGTACAAACAAAATCCCGAAATGTTCAAACAGACAGCTCGACTTTGG
GCACATGTGTATGCTGGAGCACCAGTTTCTAGTCCAGAATACACCAAAAA
AATAGAAAACCTATGTGCTATGGGCTTTGATAGGAATGCAGTAATAGTGG
CCTTGTCTTCAAAATCATGGGATGTAGAGACTGCAACAGAATTGCTTCTG
AGTAACTGAGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN


Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial expression construct for Ubc1 (E2-25K) with N-terminal GST tag (contains preScission protease site)

Estimated MW of expressed protein : 49231 Daltons
100042 R 1000 1001 1047 19007
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