Optineurin | Ubiquitylation Resource

Plasmid

pGEX6P-1-Optineurin 691-692 AG E50K

Parent Plasmid

pGEX6P1

Expressed

GST Optineurin 691-692AG E50K

DU Number

DU12185

Genbank

NP_001008212

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGTCCCATCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAG
CCCCAGTGAAAGCACAGGAAATGGACCCCCCCACCTGGCCCACCCAAACC
TGGACACGTTTACCCCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTG
ACCAAGAACCACCAGCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCAT
GAAAGGGAGATTTGAGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAG
AACGCCAGTTTTTTGAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAATG
GCCTTGAGTCATGAGAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAA
AGGGAAATCAGAAAGGTCATCTGAGGACCCCACTGATAGGACTCCAGGCT
TCCCAGGGCCGAAGCGGAGCAGGAAAAGGACCAGCTCAGGACCCAGGTGG
TGAGGCTACAAGCAGAGAAGGCAGACCTGTTGGGCATCGTGTCTGAACTG
CAGCTCAAGCTGAACTCCAGCGGCTCCTCAGAAGATTCCTTTGTTGAAAT
TAGGATGGCTGAAGGAGAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATA
GTCCTGGGCCCACGAGAACAGTCTCCACTGGCACGGCATTGTCTAAATAT
AGGAGCAGATCTGCAGATGGGGCCAAGAATTACTTCGAACATGAGGAGTT
AACTGTGAGCCAGCTCCTGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGG
AGAGACTTGAAGTTGCACTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTT
GAAAAGAAAACAAGTAATCGTTCTGAGATTGAAACCCAGACAGAGGGGAG
CACAGAGAAAGAGAATGATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCG
AAGTGGAAGCACTGAACCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAA
GAGGCTCATACAAAACTCAGCGAAGCTGAGCTAATGAAGAAGAGACTTCA
AGAAAAGTGTCAGGCCCTTGAAAGGAAAAATTCTGCAATTCCATCAGAGT
TGAATGAAAAGCAAGAGCTTGTTTATACTAACAAAAAGTTAGAGCTACAA
GTGGAAAGCATGCTATCAGAAATCAAAATGGAACAGGCTAAAACAGAGGA
TGAAAAGTCCAAATTAACTGTGCTACAGATGACACACAACAAGCTTCTTC
AAGAACATAATAATGCATTGAAAACAATTGAGGAACTAACAAGAAAAGAG
TCAGAAAAAGTGGACAGGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGA
ACTGGCAGAGAAGGCTCTGGCTTCCAAACAGCTGCAAATGGATGAAATGA
AGCAAACCATTGCCAAGCAGGAAGAGGACCTGGAAACCATGACCATCCTC
AGGGCTCAGATGGAAGTTTACTGTTCTAATTTTCATGCTGAAAGAGCAGC
GAGAGAGAAAATTCATGAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAG
TTCTGCTGAAAGAGAATGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTG
ATGGAGATGCAGAGTCGTCATGGGGCGAGAACAAGTGACTCTGACCAGCA
GGCTTACCTTGTTCAAAGAGGAGCTGAGGACAGGGACTGGCGGCAACAGC
GGAATATTCCGATTCATTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGAC
ATAGACACGTTACAGATTCACGTGATGGATTGCATCATTTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSHQPLSCLTEKEDSPSES
TGNGPPHLAHPNLDTFTPEELLQQMKELLTKNHQLKEAMKLNNQAMKGRF
EELSAWTEKQKEERQFFEIQSKEAKERLMALSHENEKLKEELGKLKGKSE
RSSEDPTDRTPGFPGPKRSRKRTSSGPRW*

Vector Type

Bacterial

Antibiotic

Amp

Comments

N ter GST for bacterial expression.

Estimated MW of expressed protein : 43837 Daltons

100042 R 1000 1001 1047 19007