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Synonyms
Ubiquitin-Activating Enzyme E1
Plasmid
pFastBac HTb Ube1
Parent Plasmid
pFastBac HTb
Expressed
6His TEV UBE1
DU Number
DU32888
Genbank
AAA61246.1
Tag
6His
Species
Human
Sequence of Insert
View Sequence
GGATCCTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGA
TCCAAAGCCGGGTTCTAACTGCTCCCCTGCCCAGTCCGTGTTGTCCGAAG
TGCCCTCGGTGCCAACCAACGGAATGGCCAAGAACGGCAGTGAAGCAGAC
ATAGACGAGGGCCTTTACTCCCGGCAGCTGTATGTGTTGGGCCATGAGGC
AATGAAGCGGCTCCAGACATCCAGTGTCCTGGTATCAGGCCTGCGGGGCC
TGGGCGTGGAGATCGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTT
ACCCTACATGACCAGGGCACTGCCCAGTGGGCTGATCTTTCCTCCCAGTT
CTACCTGCGGGAGGAGGACATCGGTAAAAACCGGGCCGAGGTATCACAGC
CCCGCCTCGCTGAGCTCAACAGCTATGTGCCTGTCACTGCCTACACTGGA
CCCCTCGTTGAGGACTTCCTTAGTGGTTTCCAGGTGGTGGTGCTCACCAA
CACCCCCCTGGAGGACCAGCTGCGAGTGGGTGAGTTCTGTCACAACCGTG
GCATCAAGCTGGTGGTGGCAGACACGCGGGGCCTGTTTGGGCAGCTCTTC
TGTGACTTTGGAGAGGAAATGATCCTCACAGATTCCAATGGGGAGCAGCC
ACTCAGTGCTATGGTTTCTATGGTTACCAAGGACAACCCCGGTGTGGTTA
CCTGCCTGGATGAGGCCCGACACGGGTTTGAGAGCGGGGACTTTGTCTCC
TTTTCAGAAGTACAGGGCATGGTTGAACTCAACGGAAATCAGCCCATGGA
GATCAAAGTCCTGGGTCCTTATACCTTTAGCATCTGTGACACCTCCAACT
TCTCCGACTACATCCGTGGAGGCATCGTCAGTCAGGTCAAAGTACCTAAG
AAGATTAGCTTTAAATCCTTGGTGGCCTCACTGGCAGAACCTGACTTTGT
GGTGACGGACTTCGCCAAGTTTTCTCGCCCTGCCCAGCTGCACATTGGCT
TCCAGGCCCTGCACCAGTTCTGTGCTCAGCATGGCCGGCCACCTCGGCCC
CGCAATGAGGAGGATGCAGCAGAACTGGTAGCCTTAGCACAGGCTGTGAA
TGCTCGAGCCCTGCCAGCAGTGCAGCAAAATAACCTGGACGAGGACCTCA
TCCGGAAGCTGGCATATGTGGCTGCTGGGGATCTGGCACCCATAAACGCC
TTCATTGGGGGCCTGGCTGCCCAGGAAGTCATGAAGGCCTGCTCCGGGAA
GTTCATGCCCATCATGCAGTGGCTATACTTTGATGCCCTTGAGTGTCTCC
CTGAGGACAAAGAGGTCCTCACAGAGGACAAGTGCCTCCAGCGCCAGAAC
CGTTATGACGGGCAAGTGGCTGTGTTTGGCTCAGACCTGCAAGAGAAGCT
GGGCAAGCAGAAGTATTTCCTGGTGGGTGCGGGGGCCATTGGCTGTGAGC
TGCTCAAGAACTTTGCCATGATTGGGCTGGGCTGCGGGGAGGGTGGAGAA
ATCATCGTTACAGACATGGACACCATTGAGAAGTCAAATCTGAATCGACA
GTTTCTTTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCTG
CTGCAGCTGTGCGCCAAATGAATCCACATATCCGGGTGACAAGCCACCAG
AACCGTGTGGGTCCTGACACGGAGCGCATCTATGATGACGATTTTTTCCA
AAACCTAGATGGCGTGGCCAATGCCCTGGACAACGTGGATGCCCGCATGT
ACATGGACCGCCGCTGTGTCTACTACCGGAAGCCACTGCTGGAGTCAGGC
ACACTGGGCACCAAGGGCAATGTGCAGGTGGTGATCCCCTTCCTGACAGA
GTCGTACAGTTCCAGCCAGGACCCACCTGAGAAGTCCATCCCCATCTGTA
CCCTGAAGAACTTCCCTAATGCCATCGAGCACACCCTGCAGTGGGCTCGG
GATGAGTTTGAAGGCCTCTTCAAGCAGCCAGCAGAAAATGTCAACCAGTA
CCTCACAGACCCCAAGTTTGTGGAGCGAACACTGCGGCTGGCAGGCACTC
AGCCCTTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGCTGCAGCGA
CCACAGACCTGGGCTGACTGCGTGACCTGGGCCTGCCACCACTGGCACAC
CCAGTACTCGAACAACATCCGGCAGCTGCTGCACAACTTCCCTCCTGACC
AGCTCACAAGCTCAGGAGCGCCGTTCTGGTCTGGGCCCAAACGCTGTCCA
CACCCGCTCACCTTTGATGTCAACAATCCCCTGCATCTGGACTATGTGAT
GGCTGCTGCCAACCTGTTTGCCCAGACCTACGGGCTGACAGGCTCTCAGG
ACCGAGCTGCTGTGGCCACATTCCTGCAGTCTGTGCAGGTCCCCGAATTC
ACCCCCAAGTCTGGCGTCAAGATCCATGTTTCTGACCAGGAGCTGCAGAG
CGCCAATGCCTCTGTTGATGACAGTCGTCTAGAGGAGCTCAAAGCCACTC
TGCCCAGCCCAGACAAGCTCCCTGGATTCAAGATGTACCCCATTGACTTT
GAGAAGGATGATGACAGCAACTTTCATATGGATTTCATCGTGGCTGCATC
CAACCTCCGGGCAGAAAACTATGACATTCCTTCTGCAGACCGGCACAAGA
GCAAGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACGACCACAGCA
GCCGTGGTTGGCCTTGTGTGTCTGGAGCTGTACAAGGTTGTGCAGGGGCA
CCGACAGCTTGACTCCTACAAGAATGGTTTCCTCAACTTGGCCCTGCCTT
TCTTTGGTTTCTCTGAACCCCTTGCCGCACCACGTCACCAGTACTATAAC
CAAGAGTGGACATTGTGGGATCGCTTTGAGGTACAAGGGCTGCAGCCTAA
TGGTGAGGAGATGACCCTCAAACAGTTCCTCGACTATTTTAAGACAGAGC
ACAAATTAGAGATCACCATGCTGTCCCAGGGCGTGTCCATGCTCTATTCC
TTCTTCATGCCAGCTGCCAAGCTCAAGGAACGGTTGGATCAGCCGATGAC
AGAGATTGTGAGCCGTGTGTCGAAGCGAAAGCTGGGCCGCCACGTGCGGG
CGCTGGTGCTTGAGCTGTGCTGTAACGACGAGAGCGGCGAGGATGTCGAG
GTTCCCTATGTCCGATACACCATCCGCTGAGGATCC
TCCAAAGCCGGGTTCTAACTGCTCCCCTGCCCAGTCCGTGTTGTCCGAAG
TGCCCTCGGTGCCAACCAACGGAATGGCCAAGAACGGCAGTGAAGCAGAC
ATAGACGAGGGCCTTTACTCCCGGCAGCTGTATGTGTTGGGCCATGAGGC
AATGAAGCGGCTCCAGACATCCAGTGTCCTGGTATCAGGCCTGCGGGGCC
TGGGCGTGGAGATCGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTT
ACCCTACATGACCAGGGCACTGCCCAGTGGGCTGATCTTTCCTCCCAGTT
CTACCTGCGGGAGGAGGACATCGGTAAAAACCGGGCCGAGGTATCACAGC
CCCGCCTCGCTGAGCTCAACAGCTATGTGCCTGTCACTGCCTACACTGGA
CCCCTCGTTGAGGACTTCCTTAGTGGTTTCCAGGTGGTGGTGCTCACCAA
CACCCCCCTGGAGGACCAGCTGCGAGTGGGTGAGTTCTGTCACAACCGTG
GCATCAAGCTGGTGGTGGCAGACACGCGGGGCCTGTTTGGGCAGCTCTTC
TGTGACTTTGGAGAGGAAATGATCCTCACAGATTCCAATGGGGAGCAGCC
ACTCAGTGCTATGGTTTCTATGGTTACCAAGGACAACCCCGGTGTGGTTA
CCTGCCTGGATGAGGCCCGACACGGGTTTGAGAGCGGGGACTTTGTCTCC
TTTTCAGAAGTACAGGGCATGGTTGAACTCAACGGAAATCAGCCCATGGA
GATCAAAGTCCTGGGTCCTTATACCTTTAGCATCTGTGACACCTCCAACT
TCTCCGACTACATCCGTGGAGGCATCGTCAGTCAGGTCAAAGTACCTAAG
AAGATTAGCTTTAAATCCTTGGTGGCCTCACTGGCAGAACCTGACTTTGT
GGTGACGGACTTCGCCAAGTTTTCTCGCCCTGCCCAGCTGCACATTGGCT
TCCAGGCCCTGCACCAGTTCTGTGCTCAGCATGGCCGGCCACCTCGGCCC
CGCAATGAGGAGGATGCAGCAGAACTGGTAGCCTTAGCACAGGCTGTGAA
TGCTCGAGCCCTGCCAGCAGTGCAGCAAAATAACCTGGACGAGGACCTCA
TCCGGAAGCTGGCATATGTGGCTGCTGGGGATCTGGCACCCATAAACGCC
TTCATTGGGGGCCTGGCTGCCCAGGAAGTCATGAAGGCCTGCTCCGGGAA
GTTCATGCCCATCATGCAGTGGCTATACTTTGATGCCCTTGAGTGTCTCC
CTGAGGACAAAGAGGTCCTCACAGAGGACAAGTGCCTCCAGCGCCAGAAC
CGTTATGACGGGCAAGTGGCTGTGTTTGGCTCAGACCTGCAAGAGAAGCT
GGGCAAGCAGAAGTATTTCCTGGTGGGTGCGGGGGCCATTGGCTGTGAGC
TGCTCAAGAACTTTGCCATGATTGGGCTGGGCTGCGGGGAGGGTGGAGAA
ATCATCGTTACAGACATGGACACCATTGAGAAGTCAAATCTGAATCGACA
GTTTCTTTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCTG
CTGCAGCTGTGCGCCAAATGAATCCACATATCCGGGTGACAAGCCACCAG
AACCGTGTGGGTCCTGACACGGAGCGCATCTATGATGACGATTTTTTCCA
AAACCTAGATGGCGTGGCCAATGCCCTGGACAACGTGGATGCCCGCATGT
ACATGGACCGCCGCTGTGTCTACTACCGGAAGCCACTGCTGGAGTCAGGC
ACACTGGGCACCAAGGGCAATGTGCAGGTGGTGATCCCCTTCCTGACAGA
GTCGTACAGTTCCAGCCAGGACCCACCTGAGAAGTCCATCCCCATCTGTA
CCCTGAAGAACTTCCCTAATGCCATCGAGCACACCCTGCAGTGGGCTCGG
GATGAGTTTGAAGGCCTCTTCAAGCAGCCAGCAGAAAATGTCAACCAGTA
CCTCACAGACCCCAAGTTTGTGGAGCGAACACTGCGGCTGGCAGGCACTC
AGCCCTTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGCTGCAGCGA
CCACAGACCTGGGCTGACTGCGTGACCTGGGCCTGCCACCACTGGCACAC
CCAGTACTCGAACAACATCCGGCAGCTGCTGCACAACTTCCCTCCTGACC
AGCTCACAAGCTCAGGAGCGCCGTTCTGGTCTGGGCCCAAACGCTGTCCA
CACCCGCTCACCTTTGATGTCAACAATCCCCTGCATCTGGACTATGTGAT
GGCTGCTGCCAACCTGTTTGCCCAGACCTACGGGCTGACAGGCTCTCAGG
ACCGAGCTGCTGTGGCCACATTCCTGCAGTCTGTGCAGGTCCCCGAATTC
ACCCCCAAGTCTGGCGTCAAGATCCATGTTTCTGACCAGGAGCTGCAGAG
CGCCAATGCCTCTGTTGATGACAGTCGTCTAGAGGAGCTCAAAGCCACTC
TGCCCAGCCCAGACAAGCTCCCTGGATTCAAGATGTACCCCATTGACTTT
GAGAAGGATGATGACAGCAACTTTCATATGGATTTCATCGTGGCTGCATC
CAACCTCCGGGCAGAAAACTATGACATTCCTTCTGCAGACCGGCACAAGA
GCAAGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACGACCACAGCA
GCCGTGGTTGGCCTTGTGTGTCTGGAGCTGTACAAGGTTGTGCAGGGGCA
CCGACAGCTTGACTCCTACAAGAATGGTTTCCTCAACTTGGCCCTGCCTT
TCTTTGGTTTCTCTGAACCCCTTGCCGCACCACGTCACCAGTACTATAAC
CAAGAGTGGACATTGTGGGATCGCTTTGAGGTACAAGGGCTGCAGCCTAA
TGGTGAGGAGATGACCCTCAAACAGTTCCTCGACTATTTTAAGACAGAGC
ACAAATTAGAGATCACCATGCTGTCCCAGGGCGTGTCCATGCTCTATTCC
TTCTTCATGCCAGCTGCCAAGCTCAAGGAACGGTTGGATCAGCCGATGAC
AGAGATTGTGAGCCGTGTGTCGAAGCGAAAGCTGGGCCGCCACGTGCGGG
CGCTGGTGCTTGAGCTGTGCTGTAACGACGAGAGCGGCGAGGATGTCGAG
GTTCCCTATGTCCGATACACCATCCGCTGAGGATCC
Amino Acid Sequence
View Sequence
MSYYHHHHHHDYDIPTTENLYFQGAMGSSSSPLSKKRRVSGPDPKPGSNC
SPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTS
SVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDI
GKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQL
RVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSM
VTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPY
TFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKF
SRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAV
QQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQW
LYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFL
VGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWD
VTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVAN
ALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQD
PPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFV
ERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIR
QLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFA
QTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDD
SRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENY
DIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYK
NGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLK
QFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVS
KRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR*
SPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTS
SVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDI
GKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQL
RVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSM
VTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPY
TFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKF
SRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAV
QQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQW
LYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFL
VGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWD
VTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVAN
ALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQD
PPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFV
ERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIR
QLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFA
QTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDD
SRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENY
DIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYK
NGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLK
QFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVS
KRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
Nter 6HIS for baculovirus expression
100042 R 1000 1001 1047 19007
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