USP7

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Plasmid
pET156P USP7
Parent Plasmid
pET15b 6His 3C
Expressed
6His USP7
DU Number
DU15644
Genbank
NP_003461.2
Tag
6His
Species
Human
Sequence of Insert
GGATCCATGAACCACCAGCAGCAGCAGCAGCAGCAGAAAGCGGGCGAGCAGCAGTTGAGCGAGCCCGAGGACATGGAGATGGAAGCGGGAGATACAGATGACCCACCAAGAATTACTCAGAACCCTGTGATCAATGGGAATGTGGCCCTGAGTGATGGACACAACACCGCGGAGGAGGACATGGAGGATGACACCAGTTGGCGCTCCGAGGCAACCTTTCAGTTCACTGTGGAGCGCTTCAGCAGACTGAGTGAGTCGGTCCTTAGCCCTCCGTGTTTTGTGCGAAATCTGCCATGGAAGATTATGGTGATGCCACGCTTTTATCCAGACAGACCACACCAAAAAAGCGTAGGATTCTTTCTCCAGTGCAATGCTGAATCTGATTCCACGTCATGGTCTTGCCATGCACAAGCAGTGCTGAAGATAATAAATTACAGAGATGATGAAAAGTCGTTCAGTCGTCGTATTAGTCATTTGTTCTTCCATAAAGAAAATGATTGGGGATTTTCCAATTTTATGGCCTGGAGTGAAGTGACCGATCCTGAGAAAGGATTTATAGATGATGACAAAGTTACCTTTGAAGTCTTTGTACAGGCGGATGCTCCCCATGGAGTTGCGTGGGATTCAAAGAAGCACACAGGCTACGTCGGCTTAAAGAATCAGGGAGCGACTTGTTACATGAACAGCCTGCTACAGACGTTATTTTTCACGAATCAGCTACGAAAGGCTGTGTACATGATGCCAACCGAGGGGGATGATTCGTCTAAAAGCGTCCCTTTAGCATTACAAAGAGTGTTCTATGAATTACAGCATAGTGATAAACCTGTAGGAACAAAAAAGTTAACAAAGTCATTTGGGTGGGAAACTTTAGATAGCTTCATGCAACATGATGTTCAGGAGCTTTGTCGAGTGTTGCTCGATAATGTGGAAAATAAGATGAAAGGCACCTGTGTAGAGGGCACCATACCCAAATTATTCCGCGGCAAAATGGTGTCCTATATCCAGTGTAAAGAAGTAGACTATCGGTCTGATAGAAGAGAAGATTATTATGATATCCAGCTAAGTATCAAAGGAAAGAAAAATATATTTGAATCATTTGTGGATTATGTGGCAGTAGAACAGCTCGATGGGGACAATAAATACGACGCTGGGGAACATGGCTTACAGGAAGCAGAGAAAGGTGTGAAATTCCTAACATTGCCACCAGTGTTACATCTACAACTGATGAGATTTATGTATGACCCTCAGACGGACCAAAATATCAAGATCAATGATAGGTTTGAATTCCCAGAGCAGTTACCACTTGATGAATTTTTGCAAAAAACAGATCCTAAGGACCCTGCAAATTATATTCTTCATGCAGTCCTGGTTCATAGTGGAGATAATCATGGTGGACATTATGTGGTTTATCTAAACCCCAAAGGGGATGGCAAATGGTGTAAATTTGATGACGACGTGGTGTCAAGGTGTACTAAAGAGGAAGCAATTGAGCACAATTATGGGGGTCACGATGACGACCTGTCTGTTCGACACTGCACTAATGCTTACATGTTAGTCTACATCAGGGAATCAAAACTGAGTGAAGTTTTACAGGCGGTCACCGACCATGATATTCCTCAGCAGTTGGTGGAGCGATTACAAGAAGAGAAAAGGATCGAGGCTCAGAAGCGGAAGGAGCGGCAGGAAGCCCATCTCTATATGCAAGTGCAGATAGTCGCAGAGGACCAGTTTTGTGGCCACCAAGGGAATGACATGTACGATGAAGAAAAAGTGAAATACACTGTGTTCAAAGTATTGAAGAACTCCTCGCTTGCTGAGTTTGTTCAGAGCCTCTCTCAGACCATGGGATTTCCACAAGATCAAATTCGATTGTGGCCCATGCAAGCAAGGAGTAATGGAACAAAACGACCAGCAATGTTAGATAATGAAGCCGACGGCAATAAAACAATGATTGAGCTCAGTGATAATGAAAACCCTTGGACAATATTCCTGGAAACAGTTGATCCCGAGCTGGCTGCTAGTGGAGCGACCTTACCCAAGTTTGATAAAGATCATGATGTAATGTTATTTTTGAAGATGTATGATCCCAAAACGCGGAGCTTGAATTACTGTGGGCATATCTACACACCAATATCCTGTAAAATACGTGACTTGCTCCCAGTTATGTGTGACAGAGCAGGATTTATTCAAGATACTAGCCTTATCCTCTATGAGGAAGTTAAACCGAATTTAACAGAGAGAATTCAGGACTATGACGTGTCTCTTGATAAAGCCCTTGATGAACTAATGGATGGTGACATCATAGTATTTCAGAAGGATGACCCTGAAAATGATAACAGTGAATTACCCACCGCAAAGGAGTATTTCCGAGATCTCTACCACCGCGTTGATGTCATTTTCTGTGATAAAACAATCCCTAATGATCCTGGATTTGTGGTTACGTTATCAAATAGAATGAATTATTTTCAGGTTGCAAAGACAGTTGCACAGAGGCTCAACACAGATCCAATGTTGCTGCAGTTTTTCAAGTCTCAAGGTTATAGGGATGGCCCAGGTAATCCTCTTAGACATAATTATGAAGGTACTTTAAGAGATCTTCTACAGTTCTTCAAGCCTAGACAACCTAAGAAACTTTACTATCAGCAGCTTAAGATGAAAATCACAGACTTTGAGAACAGGCGAAGTTTTAAATGTATATGGTTAAACAGCCAATTTAGGGAAGAGGAAATAACACTATATCCAGACAAGCATGGGTGTGTCCGGGACCTGTTAGAAGAATGTAAAAAGGCCGTGGAGCTTGGGGAGAAAGCATCAGGGAAACTTAGGCTGCTAGAAATTGTAAGCTACAAAATCATTGGTGTTCATCAAGAAGATGAACTATTAGAATGTTTATCTCCTGCAACGAGCCGGACGTTTCGAATAGAGGAAATCCCTTTGGACCAGGTGGACATAGACAAAGAGAATGAGATGCTTGTCACAGTGGCGCATTTCCACAAAGAGGTCTTCGGAACGTTCGGAATCCCGTTTTTGCTGAGGATACACCAGGGCGAGCATTTTCGAGAAGTGATGAAGCGAATCCAGAGCCTGCTGGACATCCAGGAGAAGGAGTTTGAGAAGTTTAAATTTGCAATTGTAATGATGGGCCGACACCAGTACATAAATGAAGACGAGTATGAAGTAAATTTGAAAGACTTTGAGCCACAGCCCGGTAATATGTCTCATCCTCGGCCTTGGCTAGGGCTCGACCACTTCAACAAAGCCCCAAAGAGGAGTCGCTACACTTACCTTGAAAAGGCCATTAAAATCCATAACTGAGCGGCCGC
Amino Acid Sequence
MGSSHHHHHHSSGLEVLFQGPGSMNHQQQQQQQKAGEQQLSEPEDMEMEA
GDTDDPPRITQNPVINGNVALSDGHNTAEEDMEDDTSWRSEATFQFTVER
FSRLSESVLSPPCFVRNLPWKIMVMPRFYPDRPHQKSVGFFLQCNAESDS
TSWSCHAQAVLKIINYRDDEKSFSRRISHLFFHKENDWGFSNFMAWSEVT
DPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTGYVGLKNQGATCYMNS
LLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTK
KLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGK
MVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDN
KYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEF
PEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKW
CKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEV
LQAVTDHDIPQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAEDQFCG
HQGNDMYDEEKVKYTVFKVLKNSSLAEFVQSLSQTMGFPQDQIRLWPMQA
RSNGTKRPAMLDNEADGNKTMIELSDNENPWTIFLETVDPELAASGATLP
KFDKDHDVMLFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCDRAGFI
QDTSLILYEEVKPNLTERIQDYDVSLDKALDELMDGDIIVFQKDDPENDN
SELPTAKEYFRDLYHRVDVIFCDKTIPNDPGFVVTLSNRMNYFQVAKTVA
QRLNTDPMLLQFFKSQGYRDGPGNPLRHNYEGTLRDLLQFFKPRQPKKLY
YQQLKMKITDFENRRSFKCIWLNSQFREEEITLYPDKHGCVRDLLEECKK
AVELGEKASGKLRLLEIVSYKIIGVHQEDELLECLSPATSRTFRIEEIPL
DQVDIDKENEMLVTVAHFHKEVFGTFGIPFLLRIHQGEHFREVMKRIQSL
LDIQEKEFEKFKFAIVMTGRHQYINEDEYEVNLKDFEPQPGNMSHPRPWL
GLDHFNKAPKRSRYTYLEKAIKIHN*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N ter 6HIS for bacterial expression.

Estimated MW of expressed protein : 130714 Daltons
100042 R 1000 1001 1047 19007
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