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A20 (mouse)

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Plasmid
pEBG6P-A20 (mouse)
Parent Plasmid
pEBG6P
Expressed
GST-A20 (mouse)
DU Number
DU8429
Genbank
AAH60221
Tag
GST
Species
Mouse
Sequence of Insert
GGATCCATGGCTGAACAACTTCTTCCTCAGGCTTTGTATTTGAGCAATAT
GCGGAAAGCTGTGAAGATACGAGAGAGAACCCCAGAAGACATTTTCAAAC
CTACCAATGGGATCATCTATCACTTTAAAACCATGCACCGATACACGCTG
GAGATGTTCAGAACATGCCAGTTTTGCCCACAGTTCCGAGAGATCATCCA
CAAAGCACTTATTGACAGAAGTGTCCAGGCTTCCCTGGAAAGCCAGAAGA
AGCTCAACTGGTGTCGTGAAGTCAGGAAGCTCGTGGCTCTGAAAACCAAT
GGTGATGGAAACTGCCTCATGCATGCAGCTTGTCAGTACATGTGGGGTGT
TCAGGATACTGACCTGGTCCTGAGGAAGGCCCTCTGCAGCACCCTTAAGG
AGACAGACACTCGGAACTTTAAATTCCGCTGGCAGCTGGAATCTCTGAAA
TCTCAGGAATTTGTGGAAACAGGACTTTGCTACGACACTCGGAACTGGAA
TGACGAATGGGACAACTTGGTCAAAATGGCATCAGCAGACACACCTGCAG
CCCGAAGTGGACTTCAGTACAATTCCCTGGAAGAAATCCACATATTTGTC
CTCAGCAACATCCTCAGAAGACCCATCATTGTCATTTCAGACAAAATGCT
AAGAAGTTTGGAATCTGGTTCCAATTTTGCTCCTTTGAAAGTGGGTGGGA
TTTATCTGCCTCTTCACTGGCCTGCCCAGGAGTGTTACAGATATCCCATC
GTCCTAGGCTATGACAGCCAGCACTTTGTACCCCTGGTGACCCTGAAGGA
CAGTGGACCTGAACTTCGCGCTGTTCCACTTGTTAACAGAGACCGGGGTA
GGTTTGAAGACTTAAAAGTTCACTTCTTGACAGATCCTGAGAATGAGATG
AAGGAAAAGCTTCTAAAGGAGTACTTGATAGTGATGGAGATCCCTGTGCA
AGGCTGGGACCACGGCACGACTCACCTGATCAACGCTGCAAAATTGGATG
AAGCTAACTTACCCAAAGAAATAAATTTGGTAGACGATTACTTTGAGCTT
GTTCAGCACGAATACAAGAAATGGCAGGAGAACAGCGATCAGGCCAGGAG
AGCGGCACATGCGCAGAACCCCTTGGAGCCTTCCACACCCCAGCTATCAC
TCATGGATATAAAATGTGAGACACCCAACTGTCCTTTCTTCATGTCCGTG
AACACTCAGCCTTTATTCCACGAATGCTCAGAGAGGCGCCAAAAGAATCA
GAGCAAGCTCCCAAAGCTGAACTCGAAGCTAGGCCCTGAAGGACTCCCAG
GCGTGGGACTTGGCTCCTCAAACTGGAGCCCCGAGGAAACCGCTGGAGGA
CCTCATTCAGCCCCACCCACAGCACCCAGCCTTTTTCTCTTCAGTGAGAC
CACTGCAATGAAGTGCAGGAGTCCTGGGTGCCCTTTTACTTTGAATGTGC
AGCATAATGGATTCTGTGAGCGTTGCCACGCCCGGCAGATTAATGCCAGC
CACACCGCAGACCCTGGAAAGTGCCAAGCCTGCCTTCAGGATGTCACTCG
GACCTTTAATGGCATCTGCAGTACCTGTTTCAAAAGGACTACAGCAGAGC
CCAGCTCCAGCCTCACTTCCAGTATCCCTGCCTCCTGTCACCAACGCTCC
AAGTCTGACCCCTCACAACTCATCCAAAGTCTCACTCCACACTCTTGCCA
CCGGACTGGAAATGTCTCTCCTTCTGGCTGCCTCTCCCAGGCTGCACGGA
CTCCAGGAGACAGAGCAGGGACAAGCAAGTGCAGGAAAGCTGGCTGCATG
TATTTTGGGACTCCAGAAAACAAGGGCTTTTGCACTCTATGTTTCATCGA
ATACAGAGAAAATAAGCAGTCTGTTACTGCCTCTGAGAAAGCTGGTTCCC
CGGCCCCCAGGTTCCAGAACAATGTCCCGTGCCTGGGCAGGGAGTGCGGC
ACACTCGGAAGCACCATGTTTGAAGGGTACTGTCAGAAGTGTTTCATCGA
AGCTCAGAACCAGAGATTCCATGAAGCAAGAAGAACGGAAGAACAGCTGA
GATCAAGCCAGCATAGAGACATGCCTCGAACTACACAGGTAGCCTCAAGG
CTGAAATGTGCCCGGGCCTCCTGCAAGAACATTCTGGCCTGTCGCAGTGA
GGAACTCTGTATGGAGTGCCAGCACCTAAGCCAACGAGTAGGTTCTGTGG
CCCACCGGGGTGAGCCCACGCCTGAAGAGCCCCCTAAACAGCGCTGCCGG
GCCCCTGCTTGTGATCACTTTGGCAATGCCAAGTGTAATGGTTACTGCAA
TGAGTGCTACCAGTTCAAGCAGATGTATGGCTAAGCGGCCGC
Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAEQLLPQALYLS
NMRKAVKIRERTPEDIFKPTNGIIYHFKTMHRYTLEMFRTCQFCPQFREI
IHKALIDRSVQASLESQKKLNWCREVRKLVALKTNGDGNCLMHAACQYMW
GVQDTDLVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYDTRN
WNDEWDNLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDK
MLRSLESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTL
KDSGPELRAVPLVNRDRGRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIP
VQGWDHGTTHLINAAKLDEANLPKEINLVDDYFELVQHEYKKWQENSDQA
RRAAHAQNPLEPSTPQLSLMDIKCETPNCPFFMSVNTQPLFHECSERRQK
NQSKLPKLNSKLGPEGLPGVGLGSSNWSPEETAGGPHSAPPTAPSLFLFS
ETTAMKCRSPGCPFTLNVQHNGFCERCHARQINASHTADPGKCQACLQDV
TRTFNGICSTCFKRTTAEPSSSLTSSIPASCHQRSKSDPSQLIQSLTPHS
CHRTGNVSPSGCLSQAARTPGDRAGTSKCRKAGCMYFGTPENKGFCTLCF
IEYRENKQSVTASEKAGSPAPRFQNNVPCLGRECGTLGSTMFEGYCQKCF
IEAQNQRFHEARRTEEQLRSSQHRDMPRTTQVASRLKCARASCKNILACR
SEELCMECQHLSQRVGSVAHRGEPTPEEPPKQRCRAPACDHFGNAKCNGY
CNECYQFKQMYG*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N terminal GST for mammalian expression.

Estimated MW of expressed protein : 115112 Daltons
100042 R 1000 1001 1047 19007
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