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IPAH9.8

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Plasmid
pGEX6P-1-IPAH9.8
Parent Plasmid
pGEX6P1
Expressed
GST IPAH9.8
DU Number
DU32826
Genbank
AY206445.1
Tag
GST
Species
Shigella flexneri
Sequence of Insert
GGATCCATGTTACCGATAAATAATAACTTTTCATTGCCCCAAAATTCTTT
TTATAACACTATTTCCGGTACATATGCTGATTACTTTTCAGCATGGGATA
AATGGGAAAAACAAGCGCTCCCCGGTGAAGAGCGTGATGAGGCTGTCTCC
CGACTTAAAGAATGTCTTATCAATAATTCCGATGAACTTCGACTGGACCG
TTTAAATCTGTCCTCGCTACCTGACAACTTACCAGCTCAGATAACGCTGC
TCAATGTATCATATAATCAATTAACTAACCTACCTGAACTGCCTGTTACG
CTAAAAAAATTATATTCCGCCAGCAATAAATTATCAGAATTGCCCGTGCT
ACCTCCTGCGCTGGAGTCACTTCAGGTACAACACAATGAGCTGGAAAACC
TGCCAGCTTTACCCGATTCGTTATTGACTATGAATATCAGCTATAACGAA
ATAGTCTCCTTACCATCGCTCCCACAGGCTCTTAAAAATCTCAGAGCGAC
CCGTAATTTCCTCACTGAGCTACCAGCATTTTCTGAGGGAAATAATCCCG
TTGTCAGAGAGTATTTTTTTGATAGAAATCAGATAAGTCATATCCCGGAA
AGCATTCTTAATCTGAGGAATGAATGTTCAATACATATTAGTGATAACCC
ATTATCATCCCATGCTCTGCAAGCCCTGCAAAGATTAACCTCTTCGCCGG
ACTACCACGGCCCACGGATTTACTTCTCCATGAGTGACGGACAACAGAAT
ACACTCCATCGCCCCCTGGCTGATGCCGTGACAGCATGGTTCCCGGAAAA
CAAACAATCTGATGTATCACAGATATGGCATGCTTTTGAACATGAAGAGC
ATGCCAACACCTTTTCCGCGTTCCTTGACCGCCTTTCCGATACCGTCTCT
GCACGCAATACCTCCGGATTCCGTGAACAGGTCGCTGCATGGCTGGAAAA
ACTCAGTGCCTCTGCGGAGCTTCGACAGCAGTCTTTCGCTGTTGCTGCTG
ATGCCACTGAGAGCTGTGAGGACCGTGTCGCGCTCACATGGAACAATCTC
CGGAAAACCCTCCTGGTCCATCAGGCATCAGAAGGCCTTTTCGATAATGA
TACCGGCGCTCTGCTCTCCCTGGGCAGGGAAATGTTCCGCCTCGAAATTC
TGGAGGACATTGCCCGGGATAAAGTCAGAACTCTCCATTTTGTGGATGAG
ATAGAAGTCTACCTGGCCTTCCAGACCATGCTCGCAGAGAAACTTCAGCT
CTCCACTGCCGTGAAGGAAATGCGTTTCTATGGCGTGTCGGGAGTGACAG
CAAATGACCTCCGCACTGCCGAAGCCATGGTCAGAAGCCGTGAAGAGAAT
GAATTTACGGACTGGTTCTCCCTCTGGGGACCATGGCATGCTGTACTGAA
GCGTACGGAAGCTGACCGCTGGGCGCTGGCAGAAGAGCAGAAATATGAGA
TGCTGGAGAATGAGTACCCTCAGAGGGTGGCTGACCGGCTGAAAGCATCA
GGTCTGAGCGGTGATGCGGATGCGGAGAGGGAAGCCGGTGCACAGGTGAT
GCGTGAGACTGAACAGCAGATTTACCGTCAGCTGACTGACGAGGTACTGG
CCCTGCGATTGCCTGAAAACGGCTCACAACTGCACCATTCATAAGCGGCC
GC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMLPINNNFSLPQNSFYNTI
SGTYADYFSAWDKWEKQALPGEERDEAVSRLKECLINNSDELRLDRLNLS
SLPDNLPAQITLLNVSYNQLTNLPELPVTLKKLYSASNKLSELPVLPPAL
ESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALKNLRATRNFL
TELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSSH
ALQALQRLTSSPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSD
VSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSAS
AELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGAL
LSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAV
KEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEA
DRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETE
QQIYRQLTDEVLALRLPENGSQLHHS*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N ter GST for bacterial expression

Estimated MW of expressed protein : 88816 Daltons
100042 R 1000 1001 1047 19007
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