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Plasmid
pGEX6P-1-FLAG Ubiquitin K27wt
Parent Plasmid
pGEX6P1
Expressed
GST-FLAG Ubiquitin K27wt
DU Number
DU12109
Genbank
P62988
Tag
GST
FLAG
Species
Human
Sequence of Insert
View Sequence
GAATTCGCCCTTGCAGATCTGCCACCATGGACTACAAGGACGACGATGAC
AAGCAAATCTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGAAGT
GGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAGGATCCAAGACAGGG
AAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTG
GAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCT
GCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG
AAGCAAATCTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGAAGT
GGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAGGATCCAAGACAGGG
AAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTG
GAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCT
GCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIRPCRSATMDYKDDDDK
QIFVRTLTGRTITLEVEPSDTIENVKARIQDREGIPPDQQRLIFAGRQLE
DGRTLSDYNIQRESTLHLVLRLRGG*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIRPCRSATMDYKDDDDK
QIFVRTLTGRTITLEVEPSDTIENVKARIQDREGIPPDQQRLIFAGRQLE
DGRTLSDYNIQRESTLHLVLRLRGG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N terminal GST/FLAG for bacterial expression.
100042 R 1000 1001 1047 19007
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