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Plasmid
pGEX6P-1-Ubiquitin K11R K29R K33R
Parent Plasmid
pGEX6P1
Expressed
GST-Ubiquitin K11R K29R K33R
DU Number
DU12217
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCCAGATCTTCGTGAAGACCCTGACTGGTAGGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAGGATCCAAGACA
GGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAC
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAGGATCCAAGACA
GGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIFVKTLTGRTITLEVEPS
DTIENVKARIQDREGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
LRLRGG
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIFVKTLTGRTITLEVEPS
DTIENVKARIQDREGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
LRLRGG
Vector Type
Bacterial
Antibiotic
Amp
Comments
N terminal GST for bacterial expression.
100042 R 1000 1001 1047 19007
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