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Plasmid
pGEX6P-1-FLAG Ubiquitin K6wt
Parent Plasmid
pGEX6P1
Expressed
GST-FLAG Ubiquitin K6wt
DU Number
DU12117
Genbank
P62988
Tag
GST
FLAG
Species
Human
Sequence of Insert
View Sequence
GAATTCGCCCTTGCAGATCTGCCACCATGGACTACAAGGACGACGATGAC
AAGCAAATCTTCGTGAAGACCCTGACTGGTAGGACCATCACTCTCGAAGT
GGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAGGATCCAAGACAGGG
AAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTG
GAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCT
GCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG
AAGCAAATCTTCGTGAAGACCCTGACTGGTAGGACCATCACTCTCGAAGT
GGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAGGATCCAAGACAGGG
AAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTG
GAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCT
GCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIRPCRSATMDYKDDDDK
QIFVKTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQLE
DGRTLSDYNIQRESTLHLVLRLRGG*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIRPCRSATMDYKDDDDK
QIFVKTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQLE
DGRTLSDYNIQRESTLHLVLRLRGG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N terminal GST/FLAG for bacterial expression.
100042 R 1000 1001 1047 19007
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