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USP16

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Plasmid
pEBG6P-USP16 Q141H E479D
Parent Plasmid
pEBG6P
Expressed
GST USP16 Q141H E479D
DU Number
DU15087
Genbank
AAH30777.1
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGGGAAAGAAACGGACAAAGGGAAAAACTGTTCCAATCGATGA
TTCCTCTGAAACTTTAGAACCTGTGTGCAGACACATTAGAAAAGGATTGG
AACAAGGTAATTTGAAAAAGGCTTTAGTGAATGTGGAATGGAATATCTGC
CAAGACTGTAAGACTGACAATAAAGTGAAAGATAAAGCTGAAGAAGAAAC
AGAAGAAAAGCCTTCAGTTTGGCTGTGTCTTAAATGTGGCCATCAGGGCT
GTGGCAGAAATTCTCAGGAGCAGCATGCCTTGAAGCACTATCTGACGCCA
AGATCTGAACCTCACTGTCTGGTTCTTAGTTTGGACAACTGGAGTGTATG
GTGTTACGTATGTGATAATGAGGTCCAGTATTGTAGTTCAAACCAGTTGG
GTCAAGTGGTTGATTATGTCAGAAAACATGCCAGCATTACAACTCCAAAG
CCAGAGAAAGATAATGGAAATATTGAACTTGAAAATAAAAAATTAGAAAA
AGAGAGTAAGAATGAACAAGAGAGAGAAAAGAAGGAAAACATGGCTAAAG
AGAATCCTCCCATGAATTCTCCTTGCCAAATAACCGTGAAAGGACTCAGT
AATTTGGGAAACACATGTTTCTTCAATGCAGTTATGCAGAACTTGTCACA
AACACCAGTGCTTAGAGAACTACTAAAAGAAGTGAAAATGTCTGGAACAA
TTGTAAAAATTGAACCACCTGATTTGGCATTAACAGAACCATTAGAAATA
AACCTTGAGCCTCCAGGCCCTCTTACTTTAGCCATGAGCCAGTTTCTTAA
TGAGATGCAAGAGACCAAAAAGGGGGTTGTGACACCGAAAGAACTCTTTT
CTCAGGTCTGTAAAAAAGCAGTGCGGTTTAAAGGCTATCAGCAGCAAGAC
AGCCAGGAGCTGCTTCGCTACTTATTGGATGGGATGAGAGCAGAAGAACA
CCAAAGAGTGAGTAAAGGAATACTTAAAGCATTTGGTAATTCTACTGAAA
AGTTGGATGAAGAACTAAAAAATAAAGTTAAAGATTATGAGAAGAAAAAA
TCAATGCCAAGTTTTGTTGACCGCATCTTTGGTGGTGAACTAACTAGTAT
GATCATGTGTGATCAATGCAGAACTGTCTCCTTGGTTCATGAATCTTTCC
TTGATTTGTCCCTCCCAGTTTTAGATGATCAGAGTGGTAAGAAAAGTGTA
AATGATAAAAATCTGAAAAAGACAGTGGAGGATGAAGATCAAGATAGTGA
GGAAGAAAAAGATAACGACAGTTACATAAAAGAGAGAAGTGATATTCCTT
CTGGAACAAGTAAGCACTTACAGAAAAAAGCAAAGAAACAAGCCAAAAAG
CAAGCCAAGAACCAACGAAGACAACAAAAAATTCAAGGAAAAGTTCTTCA
TTTAAATGATATTTGTACTATTGACCATCCTGAAGACAGTGATAATGAAG
CTGAAATGTCACTTCAAGGAGAAGTAAATATTAAATCCAACCATATTTCA
CAAGAGGGTGTTATGCATAAAGAATATTGTGTCAACCAGAAAGATTTGAA
TGGCCAAGCAAAAATGATCGAAAGTGTAACTGACAATCAAAAATCCACAG
AGGAAGTAGATATGAAAAATATCAACATGGATAATGATCTGGAGGTTTTA
ACATCTTCTCCCACTAGGAATTTAAATGGTGCCTACCTAACGGAAGGGAG
CAATGGAGAAGTGGACATTTCCAATGGTTTCAAAAACCTAAATTTGAATG
CTGCTCTTCATCCTGATGAAATAAATATAGAGATTCTGAATGATAGTCAT
ACTCCTGGAACAAAGGTGTATGAGGTTGTAAATGAAGATCCAGAAACTGC
TTTCTGTACTCTTGCAAACAGGGAAGTTTTCAATACTGATGAGTGTTCAA
TCCAACATTGTTTATATCAGTTCACCCGTAATGAGAAACTTCGAGATGCG
AATAAACTGCTTTGTGAAGTATGCACACGGAGACAGTGTAATGGACCAAA
GGCAAATATAAAAGGTGAAAGGAAGCATGTTTACACCAATGCCAAAAAGC
AGATGCTAATTTCTCTTGCTCCTCCTGTTCTTACTCTTCATTTAAAGAGA
TTTCAGCAGGCTGGTTTTAACCTACGCAAAGTTAACAAACACATAAAGTT
TCCGGAAATCTTAGATTTGGCTCCTTTTTGCACCCTTAAATGTAAGAATG
TTGCAGAAGAAAATACAAGGGTACTCTATTCCTTATATGGAGTTGTTGAA
CACAGTGGTACTATGAGGTCGGGGCATTACACTGCCTATGCCAAGGCAAG
AACCGCAAATAGTCATCTCTCTAATCTTGTTCTTCACGGTGATATTCCAC
AAGATTTTGAAATGGAATCAAAAGGGCAGTGGTTTCACATCAGCGACACA
CATGTGCAAGCTGTGCCTACAACTAAAGTACTAAACTCACAAGCGTACCT
CCTATTTTATGAGAGAATACTGTAAGCGGCCGC
Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMGKKRTKGKTVPI
DDSSETLEPVCRHIRKGLEQGNLKKALVNVEWNICQDCKTDNKVKDKAEE
ETEEKPSVWLCLKCGHQGCGRNSQEQHALKHYLTPRSEPHCLVLSLDNWS
VWCYVCDNEVQYCSSNQLGQVVDYVRKHASITTPKPEKDNGNIELENKKL
EKESKNEQEREKKENMAKENPPMNSPCQITVKGLSNLGNTCFFNAVMQNL
SQTPVLRELLKEVKMSGTIVKIEPPDLALTEPLEINLEPPGPLTLAMSQF
LNEMQETKKGVVTPKELFSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAE
EHQRVSKGILKAFGNSTEKLDEELKNKVKDYEKKKSMPSFVDRIFGGELT
SMIMCDQCRTVSLVHESFLDLSLPVLDDQSGKKSVNDKNLKKTVEDEDQD
SEEEKDNDSYIKERSDIPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQGKV
LHLNDICTIDHPEDSDNEAEMSLQGEVNIKSNHISQEGVMHKEYCVNQKD
LNGQAKMIESVTDNQKSTEEVDMKNINMDNDLEVLTSSPTRNLNGAYLTE
GSNGEVDISNGFKNLNLNAALHPDEINIEILNDSHTPGTKVYEVVNEDPE
TAFCTLANREVFNTDECSIQHCLYQFTRNEKLRDANKLLCEVCTRRQCNG
PKANIKGERKHVYTNAKKQMLISLAPPVLTLHLKRFQQAGFNLRKVNKHI
KFPEILDLAPFCTLKCKNVAEENTRVLYSLYGVVEHSGTMRSGHYTAYAK
ARTANSHLSNLVLHGDIPQDFEMESKGQWFHISDTHVQAVPTTKVLNSQA
YLLFYERIL*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter GST for mammalian expression.

Estimated MW of expressed protein : 120860 Daltons
100042 R 1000 1001 1047 19007
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