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SENP1

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Plasmid
pEGFP-C2-SENP1
Parent Plasmid
pEGFPC2
Expressed
GFP SENP1
DU Number
DU32464
Genbank
AK292060.1
Tag
GFP
Species
Human
Sequence of Insert
GAATTCATGGATGATATTGCTGATAGGATGAGGATGGATGCTGGAGAAGT
GACTTTAGTGAACCACAACTCCGTATTCAAAACCCACCTCCTGCCACAAA
CAGGTTTTCCAGAGGACCAGCTTTCGCTTTCTGACCAGCAGATTTTATCT
TCCAGGCAAGGACATTTGGACCGATCTTTTACATGTTCCACAAGAAGTGC
AGCTTATAATCCAAGCTATTACTCAGATAATCCTTCCTCAGACAGTTTTC
TTGGCTCAGGCGATTTAAGAACCTTTGGCCAGAGTGCAAATGGCCAATGG
AGAAATTCTACCCCATCGTCAAGCTCATCTTTACAAAAATCAAGAAACAG
CCGAAGTCTTTACCTCGAAACCCGAAAGACCTCAAGTGGATTATCAAACA
GTTTTGCGGGAAAGTCAAACCATCACTGCCATGTATCTGCATATGAAAAA
TCTTTTCCTATTAAACCTGTTCCAAGTCCATCTTGGAGTGGTTCATGTCG
TCGAAGTCTCTTGAGCCCCAAGAAAACTCAGAGGCGACATGTTAGTACAG
CAGAAGAGACAGTTCAAGAAGAAGAAAGAGAGATTTACAGACAGCTGCTA
CAGATGGTCACAGGGAAACAGTTTACTATAGCCAAACCCACCACACATTT
TCCTTTACACCTGTCTCGATGTCTTAGTTCCAGTAAAAATACTTTGAAAG
ACTCACTGTTTAAAAATGGAAACTCTTGTGCATCTCAGATCATTGGCTCT
GATACTTCATCATCTGGATCTGCCAGCATTTTAACTAACCAGGAACAGCT
GTCCCACAGTGTATATTCCCTATCTTCTTATACCCCAGATGTTGCATTTG
GATCCAAAGATTCTGGTACTCTTCATCATCCCCATCATCACCACTCTGTT
CCACATCAGCCAGATAACTTAGCAGCTTCAAATACACAATCTGAAGGATC
AGACTCTGTGATTTTACTGAAAGTGAAAGATTCCCAGACTCCAACTCCCA
GTTCTACTTTCTTCCAGGCAGAGCTGTGGATCAAAGAATTAACTAGTGTT
TATGATTCTCGAGCACGAGAAAGATTGCGCCAGATTGACGAACAGAAGGC
ATTGGCCTTACAGCTTCAAAACCAGAGATTGCAGGAGCGGGAACATTCAG
TACATGATTCAGTAGAACTACATCTTCGTGTACCTCTTGAAAAGGAGATT
CCTGTTACTGTTGTCCAAGAAACACAAAAAAAAGGTCATAAATTAACTGA
TAGTGAAGATGAATTTCCTGAAATTACAGAGGAAATGGAGAAAGAAATAA
AGAATGTATTTCGTAATGGGAATCAGGATGAAGTTCTCAGTGAAGCATTT
CGCCTGACCATTACACGCAAAGATATTCAAACTCTAAACCATCTGAATTG
GCTCAATGATGAGATCATCAATTTCTACATGAATATGCTGATGGAGCGAA
GTAAAGAGAAGGGCTTGCCAAGTGTGCATGCATTTAATACCTTTTTCTTC
ACTAAATTAAAAACGGCTGGTTATCAGGCAGTGAAACGTTGGACAAAGAA
AGTAGATGTATTTTCTGTTGACATTCTTTTGGTGCCCATTCACCTGGGAG
TACACTGGTGTCTAGCTGTTGTGGACTTTAGAAAGAAGAATATTACCTAT
TACGACTCCATGGGTGGGATAAACAATGAAGCCTGCAGAATACTCTTGCA
ATACCTAAAGCAAGAAAGCATTGACAAGAAAAGGAAAGAGTTTGACACCA
ATGGCTGGCAGCTTTTCAGCAAGAAAAGCCAGGAGATTCCTCAGCAGATG
AATGGAAGTGACTGTGGGATGTTTGCCTGCAAATATGCTGACTGTATTAC
CAAAGACAGACCAATCAACTTCACACAGCAACACATGCCATACTTCCGGA
AGCGGATGGTCTGGGAGATCCTCCACCGAAAACTCTTGTGAGTCGAC
Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFMDDIADRMRMDAGEVTLVNHNSVFKTHLLPQTGFPEDQLSLSDQQILS
SRQGHLDRSFTCSTRSAAYNPSYYSDNPSSDSFLGSGDLRTFGQSANGQW
RNSTPSSSSSLQKSRNSRSLYLETRKTSSGLSNSFAGKSNHHCHVSAYEK
SFPIKPVPSPSWSGSCRRSLLSPKKTQRRHVSTAEETVQEEEREIYRQLL
QMVTGKQFTIAKPTTHFPLHLSRCLSSSKNTLKDSLFKNGNSCASQIIGS
DTSSSGSASILTNQEQLSHSVYSLSSYTPDVAFGSKDSGTLHHPHHHHSV
PHQPDNLAASNTQSEGSDSVILLKVKDSQTPTPSSTFFQAELWIKELTSV
YDSRARERLRQIDEQKALALQLQNQRLQEREHSVHDSVELHLRVPLEKEI
PVTVVQETQKKGHKLTDSEDEFPEITEEMEKEIKNVFRNGNQDEVLSEAF
RLTITRKDIQTLNHLNWLNDEIINFYMNMLMERSKEKGLPSVHAFNTFFF
TKLKTAGYQAVKRWTKKVDVFSVDILLVPIHLGVHWCLAVVDFRKKNITY
YDSMGGINNEACRILLQYLKQESIDKKRKEFDTNGWQLFSKKSQEIPQQM
NGSDCGMFACKYADCITKDRPINFTQQHMPYFRKRMVWEILHRKLL*
Vector Type
Mammalian
Antibiotic
Kan
Comments
N ter GFP for mammalian expression. Sequence agrees with genbank except for E361D change which occurs in the original Hay construct.

Estimated MW of expressed protein : 101802 Daltons
100042 R 1000 1001 1047 19007
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