Ubiquitin

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Plasmid
pGFP TAPP Ubiquitin K63R
Parent Plasmid
pGFP TAP BN
Expressed
GFP-TAP-Ubiquitin K63R
DU Number
DU8215
Genbank
P62988
Tag
GFP
TAP
Species
Human
Sequence of Insert
GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCAGATCTT
CGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTG
ACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCT
CCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACG
CACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGGTCC
TCCGTCTCAGAGGTGGGTGATAAGGATCC
Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSATM
YPYDVPDYAQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQR
LIFAGKQLEDGRTLSDYNIQRESTLHLVLRLRGG**
Vector Type
Mammalian
Antibiotic
Amp
Comments
N terminal GFP/TAPP/HA tag for mammalian expression.
Estimated MW of expressed protein : 59932 Daltons
100042 R 1000 1001 1047 19007
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