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UBE20

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Plasmid
pGEX6P-1-UBE20
Parent Plasmid
pGEX6P1
Expressed
GST UBE20
DU Number
DU32144
Genbank
AAH51868
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGACCTCAGCCGACGTGATGTGGCAGGATGGCTCCGTGGAATG
CAACATCCGCTCCAACGACCTCTTCCCTGTGCACCACCTGGACAACAACG
AGTTCTGCCCTGGAGACTTCGTGGTAGATAAGCGAGTCCAGAGCTGTCCA
GACCCTGCTGTCTACGGTGTGGTACAGTCTGGGGACCACATCGGCCGTAC
CTGCATGGTGAAGTGGTTCAAGCTGAGGCCGAGTGGGGACGACGTGGAGC
TGATTGGAGAAGAGGAAGATGTGAGTGTTTACGACATTGCTGACCACCCT
GACTTTAGGTTCCGTACAACTGACATCGTCATCCGCATCGGCAATACTGA
GGATGGGGCTCCTCACAAGGAGGATGAGCCATCGGTGGGCCAGGTGGCCC
GTGTGGACGTCAGCAGCAAGGTGGAGGTGGTGTGGGCTGACAACTCAAAG
ACCATCATCCTGCCCCAGCACTTGTACAACATAGAGTCTGAGATTGAGGA
GTCAGACTACGATTCGGTAGAAGGCAGCACCAGCGGGGCATCCTCGGATG
AATGGGAAGATGATAGTGACAGCTGGGAGACGGACAATGGGCTGGTGGAG
GACGAGCACCCCAAGATAGAGGAGCCCCCCATCCCACCCCTGGAGCAGCC
GGTGGCCCCTGAGGACAAGGGAGTGGTGATCAGTGAAGAGGCAGCCACAG
CTGCCGTCCAGGGGGCTGTGGCCATGGCTGCCCCCATGGCCGGGCTGATG
GAGAAGGCTGGCAAGGACGGGCCACCCAAGAGCTTCCGGGAGTTGAAAGA
GGCCATCAAGATCCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGC
TGACGGGCTCGCCCACCTCTCCGACTGTGGAGCCTGAGAAGCCAACTCGG
GAGAAGAAGTTTCTGGATGACATCAAGAAGCTACAGGAAAACCTCAAGAA
GACCCTGGACAATGTGGCCATTGTAGAGGAGGAGAAGATGGAAGCAGTGC
CCGACGTAGAGCGCAAGGAGGACAAGCCCGAGGGGCAGTCACCTGTGAAG
GCTGAGTGGCCCAGCGAAACCCCGGTGCTGTGCCAGCAGTGTGGCGGCAA
GCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTCTCCGTACTGG
AGTTTGCACCCTCAAATCATTCTTTTAAGAAAATTGAGTTCCAGCCTCCA
GAAGCCAAGAAGTTCTTCAGCACAGTGCGGAAGGAGATGGCGCTGCTGGC
TACCTCACTGCCTGAGGGCATCATGGTCAAGACTTTTGAAGATAGAATGG
ACCTCTTCTCAGCTCTCATCAAGGGCCCCACTCGAACCCCCTACGAGGAT
GGCCTCTACTTGTTTGACATCCAGCTCCCCAACATCTACCCAGCCGTGCC
CCCCCACTTCTGCTACCTCTCCCAATGCAGTGGCCGCCTGAACCCCAACC
TGTATGACAATGGGAAGGTGTGTGTCAGCCTCCTGGGCACCTGGATTGGA
AAGGGGACAGAGAGGTGGACAAGCAAGTCCAGCCTTCTCCAGGTGCTCAT
CTCCATCCAAGGTCTGATCCTGGTAAATGAACCATACTACAACGAAGCCG
GCTTCGACAGTGACCGAGGCCTGCAGGAAGGCTATGAAAACAGTCGCTGT
TACAATGAGATGGCGCTGATCCGCGTGGTGCAGTCCATGACCCAGCTGGT
GCGGCGGCCCCCCGAGGTCTTTGAGCAGGAGATCAGGCAACACTTTAGCA
CTGGTGGCTGGCGGCTGGTGAACCGTATCGAGTCCTGGCTGGAAACCCAT
GCCCTGCTGGAGAAGGCCCAGGCACTGCCCAACGGGGTGCCCAAGGCCAG
CAGCTCGCCAGAGCCCCCAGCTGTAGCCGAGCTGTCAGACTCCGGCCAAC
AAGAACCTGAGGATGGAGGGCCAGCCCCAGGAGAGGCCTCCCAGGGCTCA
GACTCAGAGGGCGGTGCCCAGGGCCTGGCCTCAGCTAGCAGGGACCACAC
AGACCAGACTTCGGAGACCGCACCAGACGCATCGGTGCCACCCAGTGTGA
AACCAAAGAAGCGGAGAAAGAGCTACCGGAGCTTCTTACCTGAGAAGAGT
GGCTACCCTGACATCGGCTTCCCCCTCTTCCCACTTTCCAAGGGTTTCAT
CAAGAGCATCCGGGGTGTCCTGACGCAGTTCCGGGCTGCCCTGCTAGAGG
CAGGCATGCCGGAGTGCACAGAGGACAAGTAGGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMTSADVMWQDGSVECNIRS
NDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVK
WFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAP
HKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYD
SVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPE
DKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKI
LESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDN
VAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVT
FTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLP
EGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFC
YLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQG
LILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPP
EVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPE
PPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTS
ETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIR
GVLTQFRAALLEAGMPECTEDK*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N ter GST for bacterial expression

Estimated MW of expressed : 108539 Daltons
100042 R 1000 1001 1047 19007
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