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Plasmid
pGEX6P-1-FLAG Ubiquitin K29wt K33wt
Parent Plasmid
pGEX6P1
Expressed
GST-FLAG Ubiquitin K29wt K33wt
DU Number
DU8990
Genbank
P62988
Tag
GST
FLAG
Species
Human
Sequence of Insert
View Sequence
GAATTCGGATCTGCCACCATGGACTACAAGGACGACGATGACAAGCAAAT
CTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGAAGTGGAGCCGA
GTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACAAGGAAGGCATC
CCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGG
ACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGG
TCCTCCGTCTCAGAGGTGGGTGATAACTCGAG
CTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGAAGTGGAGCCGA
GTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACAAGGAAGGCATC
CCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGG
ACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGG
TCCTCCGTCTCAGAGGTGGGTGATAACTCGAG
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFGSATMDYKDDDDKQIF
VRTLTGRTITLEVEPSDTIENVRAKIQDKEGIPPDQQRLIFAGRQLEDGR
TLSDYNIQRESTLHLVLRLRGG*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFGSATMDYKDDDDKQIF
VRTLTGRTITLEVEPSDTIENVRAKIQDKEGIPPDQQRLIFAGRQLEDGR
TLSDYNIQRESTLHLVLRLRGG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N terminal GST/FLAG for bacterial expression.
100042 R 1000 1001 1047 19007
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