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UBA7

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Plasmid
pGEX6P-2-UBA7
Parent Plasmid
pGEX6P1
Expressed
GST UBA7
DU Number
DU32095
Genbank
NP_003326.2
Tag
GST
Species
Human
Sequence of Insert
GCGGCGCGATGGATGCCCTGGACGCTTCGAAGCTACTGGATGAGGAGCTG
TATTCAAGACAGCTGTATGTGCTGGGCTCACCTGCCATGCAGAGGATTCA
GGGAGCCAGGGTCCTGGTGTCAGGCCTGCAGGGCCTGGGGGCCGAGGTGG
CCAAGAACTTGGTTCTGATGGGTGTGGGCAGCCTCACTCTGCATGATCCC
CACCCCACCTGCTGGTCCGACCTGGCTGCCCAGTTTCTCCTCTCAGAGCA
GGACTTGGAAAGGAGCAGAGCCGAGGCCTCTCAAGAGCTCTTGGCTCAGC
TCAACAGAGCTGTCCAGGTCGTCGTGCACACGGGTGACATCACTGAGGAC
CTGCTGTTGGACTTCCAGGTGGTGGTGCTGACTGCTGCAAAGCTGGAGGA
GCAGCTGAAGGTGGGCACCTTGTGTCATAAGCATGGAGTTTGCTTTCTGG
CGGCTGACACCCGGGGCCTCGTGGGGCAGTTGTTCTGTGACTTTGGTGAG
GACTTCACTGTGCAGGACCCCACAGAGGCAGAACCCCTGACAGCTGCCAT
CCAGCACATCTCCCAGGGCTCCCCTGGCATTCTCACTCTGAGGAAAGGGG
CCAATACCCACTACTTCCGTGATGGAGACTTGGTGACTTTCTCGGGAATT
GAGGGAATGGTTGAGCTCAACGACTGTGATCCCCGGTCTATCCACGTGCG
GGAGGATGGGTCCCTGGAGATTGGAGACACAACAACTTTCTCTCGGTACT
TGCGTGGTGGGGCTATCACTGAAGTCAAGAGACCCAAGACTGTGAGACAT
AAGTCCCTGGACACAGCCCTGCTCCAGCCCCATGTGGTGGCCCAGAGCTC
CCAGGAAGTTCACCATGCCCACTGCCTGCATCAGGCCTTCTGTGCACTGC
ACAAGTTCCAGCACCTCCATGGCCGGCCACCCCAGCCCTGGGATCCTGTT
GATGCAGAGACTGTGGTGGGCCTGGCCCGGGACCTGGAACCACTGAAGCG
GACAGAGGAAGAGCCACTGGAAGAGCCACTGGATGAGGCCCTAGTGCGGA
CAGTCGCCCTAAGCAGTGCAGGTGTCTTGAGCCCTATGGTGGCCATGCTG
GGTGCAGTAGCTGCCCAGGAAGTGCTGAAGGCAATCTCCAGGAAGTTCAT
GCCTCTGGACCAGTGGCTTTACTTTGATGCCCTCGATTGTCTTCCGGAAG
ATGGGGAGCTCCTTCCCAGTCCTGAGGACTGTGCCCTGAGAGGCAGCCGC
TATGATGGGCAAATTGCAGTGTTTGGGGCTGGTTTTCAGGAGAAACTGAG
ACGCCAGCACTACCTCCTGGTGGGCGCTGGTGCCATTGGTTGTGAGCTGC
TCAAAGTCTTTGCCCTAGTGGGACTGGGGGCCGGGAACAGCGGGGGCTTG
ACTGTTGTTGACATGGACCACATAGAGCGCTCCAATCTCAGCCGTCAGTT
CCTCTTCAGGTCCCAGGACGTTGGTAGACCCAAGGCAGAGGTGGCTGCAG
CAGCTGCCCGGGGCCTGAACCCAGACTTACAGGTGATCCCGCTCACCTAC
CCACTGGATCCCACCACAGAGCACATCTATGGGGATAACTTTTTCTCCCG
TGTGGATGGTGTGGCTGCTGCCCTGGACAGTTTCCAGGCCCGGCGCTATG
TGGCTGCTCGTTGCACCCACTATCTGAAGCCACTGCTGGAGGCAGGCACA
TCGGGCACCTGGGGCAGTGCTACAGTATTCATGCCACATGTGACTGAGGC
CTACAGAGCCCCTGCCTCAGCTGCAGCTTCTGAGGATGCCCCCTACCCTG
TCTGTACCGTGCGGTACTTCCCTAGCACAGCCGAGCACACCCTGCAGTGG
GCCCGGCATGAGTTTGAAGAACTCTTCCGACTGTCTGCAGAGACCATCAA
CCACCACCAACAGGCACACACTTCCCTGGCAGACATGGATGAGCCACAGA
CACTCACCTTACTGAAGCCAGTGCTTGGGGTCCTGAGAGTGCGTCCACAG
AACTGGCAAGACTGTGTGGCGTGGGCTCTTGGCCACTGGAAACTCTGCTT
TCATTATGGCATCAAACAGCTGCTGAGGCACTTCCCACCTAATAAAGTGC
TTGAGGATGGAACTCCCTTCTGGTCAGGTCCCAAACAGTGTCCCCAGCCC
TTGGAGTTTGACACCAACCAAGACACACACCTCCTCTACGTACTGGCAGC
TGCCAACCTGTATGCCCAGATGCATGGGCTGCCTGGCTCACAGGACTGGA
CTGCACTCAGGGAGCTGCTGAAGCTGCTGCCACAGCCTGACCCCCAACAG
ATGGCCCCCATCTTTGCTAGTAATCTAGAGCTGGCTTCGGCTTCTGCTGA
GTTTGGCCCTGAGCAGCAGAAGGAACTGAACAAAGCCCTGGAAGTCTGGA
GTGTGGGCCCTCCCCTGAAGCCTCTGATGTTTGAGAAGGATGATGACAGC
AACTTCCATGTGGACTTTGTGGTAGCGGCAGCTAGCCTGAGATGTCAGAA
CTACGGGATTCCACCGGTCAACCGTGCCCAGAGCAAGCGAATTGTGGGCC
AGATTATCCCAGCCATTGCCACCACTACAGCAGCTGTGGCAGGCCTGTTG
GGCCTGGAGCTGTATAAGGTGGTGAGTGGGCCACGGCCTCGTAGTGCCTT
TCGCCACAGCTACCTACATCTGGCTGAAAACTACCTCATCCGCTATATGC
CTTTTGCCCCAGCCATCCAGACGTTCCATCACCTGAAGTGGACCTCTTGG
GACCGTCTGAAGGTACCAGCTGGGCAGCCTGAGAGGACCCTGGAGTCGCT
GCTGGCTCATCTTCAGGAGCAGCACGGGTTGAGGGTGAGGATCCTGCTGC
ACGGCTCAGCCCTGCTCTATGCGGCCGGATGGTCACCTGAAAAGCAGGCC
CAGCACCTGCCCCTCAGGGTGACAGAACTGGTTCAGCAGCTGACAGGCCA
GGCACCTGCTCCTGGGCAGCGGGTGTTGGTGCTAGAGCTGAGCTGTGAGG
GTGACGACGAGGACACTGCCTTCCCACCTCTGCACTATGAGCTGTAGGCG
GCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMDALDASK
LLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGS
LTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHT
GDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQL
FCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDL
VTFSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKR
PKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPP
QPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLS
PMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDC
ALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGA
GNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQ
VIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKP
LLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTA
EHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGV
LRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGP
KQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLP
QPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMF
EKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTA
AVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHH
LKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGW
SPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPL
HYEL*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N ter GST for bacterial expression

Estimated MW of expressed protein : 139409 Daltons
100042 R 1000 1001 1047 19007
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