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A20

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Plasmid
pCMV5 FLAG A20
Parent Plasmid
pCMV5 FLAG2
Expressed
FLAG A20
DU Number
DU15963
Genbank
BC114480
Tag
FLAG
Species
Human
Sequence of Insert
GCGGCCGCGATGGCTGAACAAGTCCTTCCTCAGGCTTTGTATTTGAGCAA
TATGCGGAAAGCTGTGAAGATACGGGAGAGAACTCCAGAAGACATTTTTA
AACCTACTAATGGGATCATTCATCATTTTAAAACCATGCACCGATACACA
CTGGAAATGTTCAGAACTTGCCAGTTTTGTCCTCAGTTTCGGGAGATCAT
CCACAAAGCCCTCATCGACAGAAACATCCAGGCCACCCTGGAAAGCCAGA
AGAAACTCAACTGGTGTCGAGAAGTCCGGAAGCTTGTGGCGCTGAAAACG
AACGGTGACGGCAATTGCCTCATGCATGCCACTTCTCAGTACATGTGGGG
CGTTCAGGACACAGACTTGGTACTGAGGAAGGCGCTGTTCAGCACGCTCA
AGGAAACAGACACACGCAACTTTAAATTCCGCTGGCAACTGGAGTCTCTC
AAATCTCAGGAATTTGTTGAAACGGGGCTTTGCTATGATACTCGGAACTG
GAATGATGAATGGGACAATCTTATCAAAATGGCTTCCACAGACACACCCA
TGGCCCGAAGTGGACTTCAGTACAACTCACTGGAAGAAATACACATATTT
GTCCTTTGCAACATCCTCAGAAGGCCAATCATTGTCATTTCAGACAAAAT
GCTAAGAAGTTTGGAATCAGGTTCCAATTTCGCCCCTTTGAAAGTGGGTG
GAATTTACTTGCCTCTCCACTGGCCTGCCCAGGAATGCTACAGATACCCC
ATTGTTCTCGGCTATGACAGCCATCATTTTGTACCCTTGGTGACCCTGAA
GGACAGTGGGCCTGAAATCCGAGCTGTTCCACTTGTTAACAGAGACCGGG
GAAGATTTGAAGACTTAAAAGTTCACTTTTTGACAGATCCTGAAAATGAG
ATGAAGGAGAAGCTCTTAAAAGAGTACTTAATGGTGATAGAAATCCCCGT
CCAAGGCTGGGACCATGGCACAACTCATCTCATCAATGCCGCAAAGTTGG
ATGAAGCTAACTTACCAAAAGAAATCAATCTGGTAGATGATTACTTTGAA
CTTGTTCAGCATGAGTACAAGAAATGGCAGGAAAACAGCGAGCAGGGGAG
GAGAGAGGGGCACGCCCAGAATCCCATGGAACCTTCCGTGCCCCAGCTTT
CTCTCATGGATGTAAAATGTGAAACGCCCAACTGCCCCTTCTTCATGTCT
GTGAACACCCAGCCTTTATGCCATGAGTGCTCAGAGAGGCGGCAAAAGAA
TCAAAACAAACTCCCAAAGCTGAACTCCAAGCCGGGCCCTGAGGGGCTCC
CTGGCATGGCGCTCGGGGCCTCTCGGGGAGAAGCCTATGAGCCCTTGGCG
TGGAACCCTGAGGAGTCCACTGGGGGGCCTCATTCGGCCCCACCGACAGC
ACCCAGCCCTTTTCTGTTCAGTGAGACCACTGCCATGAAGTGCAGGAGCC
CCGGCTGCCCCTTCACACTGAATGTGCAGCACAACGGATTTTGTGAACGT
TGCCACAACGCCCGGCAACTTCACGCCAGCCACGCCCCAGACCACACAAG
GCACTTGGATCCCGGGAAGTGCCAAGCCTGCCTCCAGGATGTTACCAGGA
CATTTAATGGGATCTGCAGTACTTGCTTCAAAAGGACTACAGCAGAGGCC
TCCTCCAGCCTCAGCACCAGCCTCCCTCCTTCCTGTCACCAGCGTTCCAA
GTCAGATCCCTCGCGGCTCGTCCGGAGCCCCTCCCCGCATTCTTGCCACA
GAGCTGGAAACGACGCCCCTGCTGGCTGCCTGTCTCAAGCTGCACGGACT
CCTGGGGACAGGACGGGGACGAGCAAGTGCAGAAAAGCCGGCTGCGTGTA
TTTTGGGACTCCAGAAAACAAGGGCTTTTGCACACTGTGTTTCATCGAGT
ACAGAGAAAACAAACATTTTGCTGCTGCCTCAGGGAAAGTCAGTCCCACA
GCGTCCAGGTTCCAGAACACCATTCCGTGCCTGGGGAGGGAATGCGGCAC
CCTTGGAAGCACCATGTTTGAAGGATACTGCCAGAAGTGTTTCATTGAAG
CTCAGAATCAGAGATTTCATGAGGCCAAAAGGACAGAAGAGCAACTGAGA
TCGAGCCAGCGCAGAGATGTGCCTCGAACCACACAAAGCACCTCAAGGCC
CAAGTGCGCCCGGGCCTCCTGCAAGAACATCCTGGCCTGCCGCAGCGAGG
AGCTCTGCATGGAGTGTCAGCATCCCAACCAGAGGATGGGCCCTGGGGCC
CACCGGGGTGAGCCTGCCCCCGAAGACCCCCCCAAGCAGCGTTGCCGGGC
CCCCGCCTGTGATCATTTTGGCAATGCCAAGTGCAACGGCTACTGCAACG
AATGCTTTCAGTTCAAGCAGATGTATGGCTAAGCGGCCGC
Amino Acid Sequence
MDYKDDDDKGIPGIPGSTRAAAMAEQVLPQALYLSNMRKAVKIRERTPED
IFKPTNGIIHHFKTMHRYTLEMFRTCQFCPQFREIIHKALIDRNIQATLE
SQKKLNWCREVRKLVALKTNGDGNCLMHATSQYMWGVQDTDLVLRKALFS
TLKETDTRNFKFRWQLESLKSQEFVETGLCYDTRNWNDEWDNLIKMASTD
TPMARSGLQYNSLEEIHIFVLCNILRRPIIVISDKMLRSLESGSNFAPLK
VGGIYLPLHWPAQECYRYPIVLGYDSHHFVPLVTLKDSGPEIRAVPLVNR
DRGRFEDLKVHFLTDPENEMKEKLLKEYLMVIEIPVQGWDHGTTHLINAA
KLDEANLPKEINLVDDYFELVQHEYKKWQENSEQGRREGHAQNPMEPSVP
QLSLMDVKCETPNCPFFMSVNTQPLCHECSERRQKNQNKLPKLNSKPGPE
GLPGMALGASRGEAYEPLAWNPEESTGGPHSAPPTAPSPFLFSETTAMKC
RSPGCPFTLNVQHNGFCERCHNARQLHASHAPDHTRHLDPGKCQACLQDV
TRTFNGICSTCFKRTTAEASSSLSTSLPPSCHQRSKSDPSRLVRSPSPHS
CHRAGNDAPAGCLSQAARTPGDRTGTSKCRKAGCVYFGTPENKGFCTLCF
IEYRENKHFAAASGKVSPTASRFQNTIPCLGRECGTLGSTMFEGYCQKCF
IEAQNQRFHEAKRTEEQLRSSQRRDVPRTTQSTSRPKCARASCKNILACR
SEELCMECQHPNQRMGPGAHRGEPAPEDPPKQRCRAPACDHFGNAKCNGY
CNECFQFKQMYG*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter FLAG for mammalian expression

Estimated MW of expressed protein : 91886 Daltons
100042 R 1000 1001 1047 19007
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