Ubiquitin

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Plasmid
pEBG6P-Ubiquitin K33wt
Parent Plasmid
pEBG6P
Expressed
GST-Ubiquitin K33wt
DU Number
DU12070
Genbank
P62988
Tag
GST
Species
Human
Sequence of Insert
GGATCTCAAATCTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAGCATGCAT
CTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGAGCTCGCTGATC
AGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCC
CCGTGCCTTCCTTGACCCTGGAAGGTGCCACAAGGGCGAATTCTGCAGAT
ATCCATCACACTGGCGGCCGC
Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSQIFVRTLTGRTIT
LEVEPSDTIENVRAKIQDKEGIPPDQQRLIFAGRQLEDGRTLSDYNIQRE
STLHLVLRLRGG*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N terminal GST for mammalian expression.

Estimated MW of expressed protein : 35990 Daltons
100042 R 1000 1001 1047 19007
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