OPTINEURIN | Ubiquitylation Resource

Plasmid

pCMV-HA Optineurin M98K

Parent Plasmid

pCMV5

Expressed

HA-Optineurin M98K

DU Number

DU8943

Genbank

NP_001008212

Tag

HA

Species

Human

Sequence of Insert

View Sequence

GGATCCATGTCCCATCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAG
CCCCAGTGAAAGCACAGGAAATGGACCCCCCCACCTGGCCCACCCAAACC
TGGACACGTTTACCCCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTG
ACCGAGAACCACCAGCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCAT
GAAAGGGAGATTTGAGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAG
AACGCCAGTTTTTTGAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAAAG
GCCTTGAGTCATGAGAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAA
AGGGAAATCAGAAAGGTCATCTGAGGACCCCACTGATGACTCCAGGCTTC
CCAGGGCCGAAGCGGAGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTG
AGGCTACAAGCAGAGAAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCA
GCTCAAGCTGAACTCCAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTA
GGATGGCTGAAGGAGAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGT
CCTGGGCCCACGAGAACAGTCTCCACTGGCACGGCATTGTCTAAATATAG
GAGCAGATCTGCAGATGGGGCCAAGAATTACTTCGAACATGAGGAGTTAA
CTGTGAGCCAGCTCCTGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAG
AGACTTGAAGTTGCACTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGA
AAAGAAAACAAGTAATCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCA
CAGAGAAAGAGAATGATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAA
GTGGAAGCACTGAACCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGA
GGCTCATACAAAACTCAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAG
AAAAGTGTCAGGCCCTTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTG
AATGAAAAGCAAGAGCTTGTTTATACTAACAAAAAGTTAGAGCTACAAGT
GGAAAGCATGCTATCAGAAATCAAAATGGAACAGGCTAAAACAGAGGATG
AAAAGTCCAAATTAACTGTGCTACAGATGACACACAACAAGCTTCTTCAA
GAACATAATAATGCATTGAAAACAATTGAGGAACTAACAAGAAAAGAGTC
AGAAAAAGTGGACAGGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAAC
TGGCAGAGAAGGCTCTGGCTTCCAAACAGCTGCAAATGGATGAAATGAAG
CAAACCATTGCCAAGCAGGAAGAGGACCTGGAAACCATGACCATCCTCAG
GGCTCAGATGGAAGTTTACTGTTCTGATTTTCATGCTGAAAGAGCAGCGA
GAGAGAAAATTCATGAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTT
CTGCTGAAAGAGAATGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGAT
GGAGATGCAGAGTCGTCATGGGGCGAGAACAAGTGACTCTGACCAGCAGG
CTTACCTTGTTCAAAGAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGG
AATATTCCGATTCATTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACAT
AGACACGTTACAGATTCACGTGATGGATTGCATCATTTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MYPYDVPDYAGSMSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPE
ELLQQMKELLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEI
QSKEAKERLKALSHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQ
EKDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEA
EGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLL
CLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDE
EKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALE
RKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTV
LQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALA
SKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEE
KEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRG
AEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter HA for mammalian expression.

Estimated MW of expressed protein : 67276 Daltons

100042 R 1000 1001 1047 19007