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BARD1

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Plasmid
pCMV-FLAG-2 BARD1
Parent Plasmid
pCMV5 FLAG2
Expressed
FLAG-Bard1
DU Number
DU12159
Genbank
AAB38316.1
Tag
FLAG
Species
Human
Sequence of Insert
GCGGCCGCGATGCCGGATAATCGGCAGCCGAGGAACCGGCAGCCGAGGAT
CCGCTCCGGGAACGAGCCTCGTTCCGCGCCCGCCATGGAACCGGATGGTC
GCGGTGCCTGGGCCCACAGTCGCGCCGCGCTCGACCGCCTGGAGAAGCTG
CTGCGCTGCTCGCGTTGTACTAACATTCTGAGAGAGCCTGTGTGTTTAGG
AGGATGTGAGCACATCTTCTGTAGTAATTGTGTAAGTGACTGCATTGGAA
CTGGATGTCCAGTGTGTTACACCCCGGCCTGGATACAAGACTTGAAGATA
AATAGACAACTGGACAGCATGATTCAACTTTGTAGTAAGCTTCGAAATTT
GCTACATGACAATGAGCTGTCAGATTTGAAAGAAGATAAACCTAGGAAAA
GTTTGTTTAATGATGCAGGAAACAAGAAGAATTCAATTAAAATGTGGTTT
AGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCTTCAGTGCA
AACCCAGCCTGCAATAAAAAAAGATGCAAGTGCTCAGCAAGACTCATATG
AATTTGTTTCCCCAAGTCCTCCTGCAGATGTTTCTGAGAGGGCTAAAAAG
GCTTCTGCAAGATCTGGAAAAAAGCAAAAAAAGAAAACTTTAGCTGAAAT
CAACCAAAAATGGAATTTAGAGGCAGAAAAAGAAGATGGTGAATTTGACT
CCAAAGAGGAATCTAAGCAAAAGCTGGTATCCTTCTGTAGCCAACCATCT
GTTATCTCCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGCAAGTGG
CTCCTTGACAGAATCTGAATGTTTTGGAAGTTTAACTGAAGTCTCTTTAC
CACTGGCTGAGCAAATAGAGTCTCCAGACACTAAGAGCAGGAATGAAGTA
GTGACTCCTGAGAAGGTCTGCAAAAATTATCTTACATCTAAGAAATCTTT
GCCATTAGAAAATAATGGAAAACGTGGCCATCACAATAGACTTTCCAGTC
CCATTTCTAAGAGATGTAGAACCAGCATTCTGAGCACCAGTGGAGATTTT
GTTAAGCAAACGGTGCCCTCAGAAAATATACCATTGCCTGAATGTTCTTC
ACCACCTTCATGCAAACGTAAAGTTGGTGGTACATCAGGGAGGAAAAACA
GTAACATGTCCGATGAATTCATTAGTCTTTCACCAGGTACACCACCTTCT
ACATTAAGTAGTTCAAGTTACAGGCAAGTGATGTCTAGTCCCTCAGCAAT
GAAGCTGTTGCCCAATATGGCTGTGAAAAGAAATCATAGAGGAGAGACTT
TGCTCCATATTGCTTCTATTAAGGGCGACATACCTTCTGTTGAATACCTT
TTACAAAATGGAAGTGATCCAAATGTTAAAGACCATGCTGGATGGACACC
ATTGCATGAAGCTTGCAATCATGGGCACCTGAAGGTAGTGGAATTATTGC
TCCAGCATAAGGCATTGGTGAACACCACCGGGTATCAAAATGACTCACCA
CTTCACGATGCAGCCAAGAATGGGCACGTGGATATAGTCAAGCTGTTACT
TTCCTATGGAGCCTCCAGAAATGCTGTTAATATATTTGGTCTGCGGCCTG
TCGATTATACAGATGATGAAAGTATGAAATCGCTATTGCTGCTACCAGAG
AAGAATGAATCATCCTCAGCTAGCCACTGCTCAGTAATGAACACTGGGCA
GCGTAGGGATGGACCTCTTGTACTTATAGGCAGTGGGCTGTCTTCAGAAC
AACAGAAAATGCTCAGTGAGCTTGCAGTAATTCTTAAGGCTAAAAAATAT
ACTGAGTTTGACAGTACAGTAACTCATGTTGTTGTTCCTGGTGATGCAGT
TCAAAGTACCTTGAAGTGTATGCTTGGGATTCTCAATGGATGCTGGATTC
TAAAATTTGAATGGGTAAAAGCATGTCTACGAAGAAAAGTATGTGAACAG
GAAGAAAAGTATGAAATTCCTGAAGGTCCACGCAGAAGCAGGCTCAACAG
AGAACAGCTGTTGCCAAAGCTGTTTGATGGATGCTACTTCTATTTGTGGG
GAACCTTCAAACACCATCCAAAGGACAACCTTATTAAGCTCGTCACTGCA
GGTGGGGGCCAGATCCTCAGTAGAAAGCCCAAGCCAGACAGTGACGTGAC
TCAGACCATCAATACAGTCGCATACCATGCGAGACCCGATTCTGATCAGC
GCTTCTGCACACAGTATATCATCTATGAAGATTTGTGTAATTATCACCCA
GAGAGGGTTCGGCAGGGCAAAGTCTGGAAGGCTCCTTCGAGCTGGTTTAT
AGACTGTGTGATGTCCTTTGAGTTGCTTCCTCTTGACAGCTGAGCGGCCG
C
Amino Acid Sequence
MDYKDDDDKGIPGIPGSTRAAAMPDNRQPRNRQPRIRSGNEPRSAPAMEP
DGRGAWAHSRAALDRLEKLLRCSRCTNILREPVCLGGCEHIFCSNCVSDC
IGTGCPVCYTPAWIQDLKINRQLDSMIQLCSKLRNLLHDNELSDLKEDKP
RKSLFNDAGNKKNSIKMWFSPRSKKVRYVVSKASVQTQPAIKKDASAQQD
SYEFVSPSPPADVSERAKKASARSGKKQKKKTLAEINQKWNLEAEKEDGE
FDSKEESKQKLVSFCSQPSVISSPQINGEIDLLASGSLTESECFGSLTEV
SLPLAEQIESPDTKSRNEVVTPEKVCKNYLTSKKSLPLENNGKRGHHNRL
SSPISKRCRTSILSTSGDFVKQTVPSENIPLPECSSPPSCKRKVGGTSGR
KNSNMSDEFISLSPGTPPSTLSSSSYRQVMSSPSAMKLLPNMAVKRNHRG
ETLLHIASIKGDIPSVEYLLQNGSDPNVKDHAGWTPLHEACNHGHLKVVE
LLLQHKALVNTTGYQNDSPLHDAAKNGHVDIVKLLLSYGASRNAVNIFGL
RPVDYTDDESMKSLLLLPEKNESSSASHCSVMNTGQRRDGPLVLIGSGLS
SEQQKMLSELAVILKAKKYTEFDSTVTHVVVPGDAVQSTLKCMLGILNGC
WILKFEWVKACLRRKVCEQEEKYEIPEGPRRSRLNREQLLPKLFDGCYFY
LWGTFKHHPKDNLIKLVTAGGGQILSRKPKPDSDVTQTINTVAYHARPDS
DQRFCTQYIIYEDLCNYHPERVRQGKVWKAPSSWFIDCVMSFELLPLDS*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter FLAG for mammalian expression.

Estimated MW of expressed protein : 88892 Daltons
100042 R 1000 1001 1047 19007
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