Ubiquitin

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Plasmid
pGEX6P-1-Ubiquitin I44A
Parent Plasmid
pGEX6P1
Expressed
GSt Ubiquitin I44A
DU Number
DU32715
Genbank
P62988
Tag
GST
Species
Human
Sequence of Insert
GGATCCCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGGCCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQQRLAFAGKQLEDGRTLSDYNIQKESTLHLV
LRLRGG
Vector Type
Bacterial
Antibiotic
Amp
Comments
N terminal GST for bacterial expression

Estimated MW of expressed protein : 35193 Daltons
100042 R 1000 1001 1047 19007
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