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SENP1

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Plasmid
pFBHTa SENP1
Parent Plasmid
pSC-A
Expressed
6His SENP1
DU Number
DU32813
Genbank
AK292060.1
Tag
6His
Species
Human
Sequence of Insert
GAATTCATGGATGATATTGCTGATAGGATGAGGATGGATGCTGGAGAAGT
GACTTTAGTGAACCACAACTCCGTATTCAAAACCCACCTCCTGCCACAAA
CAGGTTTTCCAGAGGACCAGCTTTCGCTTTCTGACCAGCAGATTTTATCT
TCCAGGCAAGGACATTTGGACCGATCTTTTACATGTTCCACAAGAAGTGC
AGCTTATAATCCAAGCTATTACTCAGATAATCCTTCCTCAGACAGTTTTC
TTGGCTCAGGCGATTTAAGAACCTTTGGCCAGAGTGCAAATGGCCAATGG
AGAAATTCTACCCCATCGTCAAGCTCATCTTTACAAAAATCAAGAAACAG
CCGAAGTCTTTACCTCGAAACCCGAAAGACCTCAAGTGGATTATCAAACA
GTTTTGCGGGAAAGTCAAACCATCACTGCCATGTATCTGCATATGAAAAA
TCTTTTCCTATTAAACCTGTTCCAAGTCCATCTTGGAGTGGTTCATGTCG
TCGAAGTCTCTTGAGCCCCAAGAAAACTCAGAGGCGACATGTTAGTACAG
CAGAAGAGACAGTTCAAGAAGAAGAAAGAGAGATTTACAGACAGCTGCTA
CAGATGGTCACAGGGAAACAGTTTACTATAGCCAAACCCACCACACATTT
TCCTTTACACCTGTCTCGATGTCTTAGTTCCAGTAAAAATACTTTGAAAG
ACTCACTGTTTAAAAATGGAAACTCTTGTGCATCTCAGATCATTGGCTCT
GATACTTCATCATCTGGATCTGCCAGCATTTTAACTAACCAGGAACAGCT
GTCCCACAGTGTATATTCCCTATCTTCTTATACCCCAGATGTTGCATTTG
GATCCAAAGATTCTGGTACTCTTCATCATCCCCATCATCACCACTCTGTT
CCACATCAGCCAGATAACTTAGCAGCTTCAAATACACAATCTGAAGGATC
AGACTCTGTGATTTTACTGAAAGTGAAAGATTCCCAGACTCCAACTCCCA
GTTCTACTTTCTTCCAGGCAGAGCTGTGGATCAAAGAATTAACTAGTGTT
TATGATTCTCGAGCACGAGAAAGATTGCGCCAGATTGACGAACAGAAGGC
ATTGGCCTTACAGCTTCAAAACCAGAGATTGCAGGAGCGGGAACATTCAG
TACATGATTCAGTAGAACTACATCTTCGTGTACCTCTTGAAAAGGAGATT
CCTGTTACTGTTGTCCAAGAAACACAAAAAAAAGGTCATAAATTAACTGA
TAGTGAAGATGAATTTCCTGAAATTACAGAGGAAATGGAGAAAGAAATAA
AGAATGTATTTCGTAATGGGAATCAGGATGAAGTTCTCAGTGAAGCATTT
CGCCTGACCATTACACGCAAAGATATTCAAACTCTAAACCATCTGAATTG
GCTCAATGATGAGATCATCAATTTCTACATGAATATGCTGATGGAGCGAA
GTAAAGAGAAGGGCTTGCCAAGTGTGCATGCATTTAATACCTTTTTCTTC
ACTAAATTAAAAACGGCTGGTTATCAGGCAGTGAAACGTTGGACAAAGAA
AGTAGATGTATTTTCTGTTGACATTCTTTTGGTGCCCATTCACCTGGGAG
TACACTGGTGTCTAGCTGTTGTGGACTTTAGAAAGAAGAATATTACCTAT
TACGACTCCATGGGTGGGATAAACAATGAAGCCTGCAGAATACTCTTGCA
ATACCTAAAGCAAGAAAGCATTGACAAGAAAAGGAAAGAGTTTGACACCA
ATGGCTGGCAGCTTTTCAGCAAGAAAAGCCAGGAGATTCCTCAGCAGATG
AATGGAAGTGACTGTGGGATGTTTGCCTGCAAATATGCTGACTGTATTAC
CAAAGACAGACCAATCAACTTCACACAGCAACACATGCCATACTTCCGGA
AGCGGATGGTCTGGGAGATCCTCCACCGAAAACTCTTGTGAGTCGAC
Amino Acid Sequence
MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMDDIADRMRMDAGEVTLVNH
NSVFKTHLLPQTGFPEDQLSLSDQQILSSRQGHLDRSFTCSTRSAAYNPS
YYSDNPSSDSFLGSGDLRTFGQSANGQWRNSTPSSSSSLQKSRNSRSLYL
ETRKTSSGLSNSFAGKSNHHCHVSAYEKSFPIKPVPSPSWSGSCRRSLLS
PKKTQRRHVSTAEETVQEEEREIYRQLLQMVTGKQFTIAKPTTHFPLHLS
RCLSSSKNTLKDSLFKNGNSCASQIIGSDTSSSGSASILTNQEQLSHSVY
SLSSYTPDVAFGSKDSGTLHHPHHHHSVPHQPDNLAASNTQSEGSDSVIL
LKVKDSQTPTPSSTFFQAELWIKELTSVYDSRARERLRQIDEQKALALQL
QNQRLQEREHSVHDSVELHLRVPLEKEIPVTVVQETQKKGHKLTDSEDEF
PEITEEMEKEIKNVFRNGNQDEVLSEAFRLTITRKDIQTLNHLNWLNDEI
INFYMNMLMERSKEKGLPSVHAFNTFFFTKLKTAGYQAVKRWTKKVDVFS
VDILLVPIHLGVHWCLAVVDFRKKNITYYDSMGGINNEACRILLQYLKQE
SIDKKRKEFDTNGWQLFSKKSQEIPQQMNGSDCGMFACKYADCITKDRPI
NFTQQHMPYFRKRMVWEILHRKLL*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
N ter 6HIS for insect expression. Sequence agrees with genbank except for E361D change which occurs in the original Hay construct.

Estimated MW of expressed protein : 77181 Daltons
100042 R 1000 1001 1047 19007
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