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Plasmid
pCMV5-FLAG-2 SMURF2 C716A
Parent Plasmid
pCMV5 FLAG2
Expressed
FLAG SMURF2 C716A
DU Number
DU32615
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
ATGTCTAACCCCGGAGGCCGGAGGAACGGGCCCGTCAAGCTGCGCCTGAC
AGTACTCTGTGCAAAAAACCTGGTGAAAAAGGATTTTTTCCGACTTCCTG
ATCCATTTGCTAAGGTGGTGGTTGATGGATCTGGGCAATGCCATTCTACA
GATACTGTGAAGAATACGCTTGATCCAAAGTGGAATCAGCATTATGACCT
GTATATTGGAAAGTCTGATTCAGTTACGATCAGTGTATGGAATCACAAGA
AGATCCATAAGAAACAAGGTGCTGGATTTCTCGGTTGTGTTCGTCTTCTT
TCCAATGCCATCAACCGCCTCAAAGACACTGGTTATCAGAGGTTGGATTT
ATGCAAACTCGGGCCAAATGACAATGATACAGTTAGAGGACAGATAGTAG
TAAGTCTTCAGTCCAGAGACCGAATAGGCACAGGAGGACAAGTTGTGGAC
TGCAGTCGTTTATTTGATAACGATTTACCAGACGGCTGGGAAGAAAGGAG
AACCGCCTCTGGAAGAATCCAGTATCTAAACCATATAACAAGAACTACGC
AATGGGAGCGCCCAACACGACCGGCATCCGAATATTCTAGCCCTGGCAGA
CCTCTTAGCTGCTTTGTTGATGAGAACACTCCAATTAGTGGAACAAATGG
TGCAACATGTGGACAGTCTTCAGATCCCAGGCTGGCAGAGAGGAGAGTCA
GGTCACAACGACATAGAAATTACATGAGCAGAACACATTTACATACTCCT
CCAGACCTACCAGAAGGCTATGAACAGAGGACAACGCAACAAGGCCAGGT
GTATTTCTTACATACACAGACTGGTGTGAGCACATGGCATGATCCAAGAG
TGCCCAGGGATCTTAGCAACATCAATTGTGAAGAGCTTGGTCCATTGCCT
CCTGGATGGGAGATCCGTAATACGGCAACAGGCAGAGTTTATTTCGTTGA
CCATAACAACAGAACAACACAATTTACAGATCCTCGGCTGTCTGCTAACT
TGCATTTAGTTTTAAATCGGCAGAACCAATTGAAAGACCAACAGCAACAG
CAAGTGGTATCGTTATGTCCTGATGACACAGAATGCCTGACAGTCCCAAG
GTACAAGCGAGACCTGGTTCAGAAACTAAAAATTTTGCGGCAAGAACTTT
CCCAACAACAGCCTCAGGCAGGTCATTGCCGCATTGAGGTTTCCAGGGAA
GAGATTTTTGAGGAATCATATCGACAGGTCATGAAAATGAGACCAAAAGA
TCTCTGGAAGCGATTAATGATAAAATTTCGTGGAGAAGAAGGCCTTGACT
ATGGAGGCGTTGCCAGGGAATGGTTGTATCTCTTGTCACATGAAATGTTG
AATCCATACTATGGCCTCTTCCAGTATTCAAGAGATGATATTTATACATT
GCAGATCAATCCTGATTCTGCAGTTAATCCGGAACATTTATCCTATTTCC
ACTTTGTTGGACGAATAATGGGAATGGCTGTGTTTCATGGACATTATATT
GATGGTGGTTTCACATTGCCTTTTTATAAGCAATTGCTTGGGAAGTCAAT
TACCTTGGATGACATGGAGTTAGTAGATCCGGATCTTCACAACAGTTTAG
TGTGGATACTTGAGAATGATATTACAGGTGTTTTGGACCATACCTTCTGT
GTTGAACATAATGCATATGGTGAAATTATTCAGCATGAACTTAAACCAAA
TGGCAAAAGTATCCCTGTTAATGAAGAAAATAAAAAAGAATATGTCAGGC
TCTATGTGAACTGGAGATTTTTACGAGGCATTGAGGCTCAATTCTTGGCT
CTGCAGAAAGGATTTAATGAAGTAATTCCACAACATCTGCTGAAGACATT
TGATGAGAAGGAGTTAGAGCTCATTATTTGTGGACTTGGAAAGATAGATG
TTAATGACTGGAAGGTAAACACCCGGTTAAAACACTGTACACCAGACAGC
AACATTGTCAAATGGTTCTGGAAAGCTGTGGAGTTTTTTGATGAAGAGCG
ACGAGCAAGATTGCTTCAGTTTGTGACAGGATCCTCTCGAGTGCCTCTGC
AGGGCTTCAAAGCATTGCAAGGTGCTGCAGGCCCGAGACTCTTTACCATA
CACCAGATTGATGCCTGCACTAACAACCTGCCGAAAGCCCACACTGCCTT
CAATCGAATAGACATTCCACCCTATGAAAGCTATGAAAAGCTATATGAAA
AGCTGCTAACAGCCATTGAAGAAACATGTGGATTTGCTGTGGAATGA
AGTACTCTGTGCAAAAAACCTGGTGAAAAAGGATTTTTTCCGACTTCCTG
ATCCATTTGCTAAGGTGGTGGTTGATGGATCTGGGCAATGCCATTCTACA
GATACTGTGAAGAATACGCTTGATCCAAAGTGGAATCAGCATTATGACCT
GTATATTGGAAAGTCTGATTCAGTTACGATCAGTGTATGGAATCACAAGA
AGATCCATAAGAAACAAGGTGCTGGATTTCTCGGTTGTGTTCGTCTTCTT
TCCAATGCCATCAACCGCCTCAAAGACACTGGTTATCAGAGGTTGGATTT
ATGCAAACTCGGGCCAAATGACAATGATACAGTTAGAGGACAGATAGTAG
TAAGTCTTCAGTCCAGAGACCGAATAGGCACAGGAGGACAAGTTGTGGAC
TGCAGTCGTTTATTTGATAACGATTTACCAGACGGCTGGGAAGAAAGGAG
AACCGCCTCTGGAAGAATCCAGTATCTAAACCATATAACAAGAACTACGC
AATGGGAGCGCCCAACACGACCGGCATCCGAATATTCTAGCCCTGGCAGA
CCTCTTAGCTGCTTTGTTGATGAGAACACTCCAATTAGTGGAACAAATGG
TGCAACATGTGGACAGTCTTCAGATCCCAGGCTGGCAGAGAGGAGAGTCA
GGTCACAACGACATAGAAATTACATGAGCAGAACACATTTACATACTCCT
CCAGACCTACCAGAAGGCTATGAACAGAGGACAACGCAACAAGGCCAGGT
GTATTTCTTACATACACAGACTGGTGTGAGCACATGGCATGATCCAAGAG
TGCCCAGGGATCTTAGCAACATCAATTGTGAAGAGCTTGGTCCATTGCCT
CCTGGATGGGAGATCCGTAATACGGCAACAGGCAGAGTTTATTTCGTTGA
CCATAACAACAGAACAACACAATTTACAGATCCTCGGCTGTCTGCTAACT
TGCATTTAGTTTTAAATCGGCAGAACCAATTGAAAGACCAACAGCAACAG
CAAGTGGTATCGTTATGTCCTGATGACACAGAATGCCTGACAGTCCCAAG
GTACAAGCGAGACCTGGTTCAGAAACTAAAAATTTTGCGGCAAGAACTTT
CCCAACAACAGCCTCAGGCAGGTCATTGCCGCATTGAGGTTTCCAGGGAA
GAGATTTTTGAGGAATCATATCGACAGGTCATGAAAATGAGACCAAAAGA
TCTCTGGAAGCGATTAATGATAAAATTTCGTGGAGAAGAAGGCCTTGACT
ATGGAGGCGTTGCCAGGGAATGGTTGTATCTCTTGTCACATGAAATGTTG
AATCCATACTATGGCCTCTTCCAGTATTCAAGAGATGATATTTATACATT
GCAGATCAATCCTGATTCTGCAGTTAATCCGGAACATTTATCCTATTTCC
ACTTTGTTGGACGAATAATGGGAATGGCTGTGTTTCATGGACATTATATT
GATGGTGGTTTCACATTGCCTTTTTATAAGCAATTGCTTGGGAAGTCAAT
TACCTTGGATGACATGGAGTTAGTAGATCCGGATCTTCACAACAGTTTAG
TGTGGATACTTGAGAATGATATTACAGGTGTTTTGGACCATACCTTCTGT
GTTGAACATAATGCATATGGTGAAATTATTCAGCATGAACTTAAACCAAA
TGGCAAAAGTATCCCTGTTAATGAAGAAAATAAAAAAGAATATGTCAGGC
TCTATGTGAACTGGAGATTTTTACGAGGCATTGAGGCTCAATTCTTGGCT
CTGCAGAAAGGATTTAATGAAGTAATTCCACAACATCTGCTGAAGACATT
TGATGAGAAGGAGTTAGAGCTCATTATTTGTGGACTTGGAAAGATAGATG
TTAATGACTGGAAGGTAAACACCCGGTTAAAACACTGTACACCAGACAGC
AACATTGTCAAATGGTTCTGGAAAGCTGTGGAGTTTTTTGATGAAGAGCG
ACGAGCAAGATTGCTTCAGTTTGTGACAGGATCCTCTCGAGTGCCTCTGC
AGGGCTTCAAAGCATTGCAAGGTGCTGCAGGCCCGAGACTCTTTACCATA
CACCAGATTGATGCCTGCACTAACAACCTGCCGAAAGCCCACACTGCCTT
CAATCGAATAGACATTCCACCCTATGAAAGCTATGAAAAGCTATATGAAA
AGCTGCTAACAGCCATTGAAGAAACATGTGGATTTGCTGTGGAATGA
Amino Acid Sequence
View Sequence
DYKDDDDKGIPGIPGSTRAAAMSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTAFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE
Vector Type
Mammalian
Antibiotic
Amp
Comments
Estimated MW of expressed protein : 88322.08 Daltons
100042 R 1000 1001 1047 19007
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