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BARD1

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Plasmid
pEBG6P-BARD1
Parent Plasmid
pEBG6P
Expressed
GST-BARD1
DU Number
DU12160
Genbank
AAB38316.1
Tag
GST
Species
Human
Sequence of Insert
GCGGCCGCGATGCCGGATAATCGGCAGCCGAGGAACCGGCAGCCGAGGAT
CCGCTCCGGGAACGAGCCTCGTTCCGCGCCCGCCATGGAACCGGATGGTC
GCGGTGCCTGGGCCCACAGTCGCGCCGCGCTCGACCGCCTGGAGAAGCTG
CTGCGCTGCTCGCGTTGTACTAACATTCTGAGAGAGCCTGTGTGTTTAGG
AGGATGTGAGCACATCTTCTGTAGTAATTGTGTAAGTGACTGCATTGGAA
CTGGATGTCCAGTGTGTTACACCCCGGCCTGGATACAAGACTTGAAGATA
AATAGACAACTGGACAGCATGATTCAACTTTGTAGTAAGCTTCGAAATTT
GCTACATGACAATGAGCTGTCAGATTTGAAAGAAGATAAACCTAGGAAAA
GTTTGTTTAATGATGCAGGAAACAAGAAGAATTCAATTAAAATGTGGTTT
AGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCTTCAGTGCA
AACCCAGCCTGCAATAAAAAAAGATGCAAGTGCTCAGCAAGACTCATATG
AATTTGTTTCCCCAAGTCCTCCTGCAGATGTTTCTGAGAGGGCTAAAAAG
GCTTCTGCAAGATCTGGAAAAAAGCAAAAAAAGAAAACTTTAGCTGAAAT
CAACCAAAAATGGAATTTAGAGGCAGAAAAAGAAGATGGTGAATTTGACT
CCAAAGAGGAATCTAAGCAAAAGCTGGTATCCTTCTGTAGCCAACCATCT
GTTATCTCCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGCAAGTGG
CTCCTTGACAGAATCTGAATGTTTTGGAAGTTTAACTGAAGTCTCTTTAC
CACTGGCTGAGCAAATAGAGTCTCCAGACACTAAGAGCAGGAATGAAGTA
GTGACTCCTGAGAAGGTCTGCAAAAATTATCTTACATCTAAGAAATCTTT
GCCATTAGAAAATAATGGAAAACGTGGCCATCACAATAGACTTTCCAGTC
CCATTTCTAAGAGATGTAGAACCAGCATTCTGAGCACCAGTGGAGATTTT
GTTAAGCAAACGGTGCCCTCAGAAAATATACCATTGCCTGAATGTTCTTC
ACCACCTTCATGCAAACGTAAAGTTGGTGGTACATCAGGGAGGAAAAACA
GTAACATGTCCGATGAATTCATTAGTCTTTCACCAGGTACACCACCTTCT
ACATTAAGTAGTTCAAGTTACAGGCAAGTGATGTCTAGTCCCTCAGCAAT
GAAGCTGTTGCCCAATATGGCTGTGAAAAGAAATCATAGAGGAGAGACTT
TGCTCCATATTGCTTCTATTAAGGGCGACATACCTTCTGTTGAATACCTT
TTACAAAATGGAAGTGATCCAAATGTTAAAGACCATGCTGGATGGACACC
ATTGCATGAAGCTTGCAATCATGGGCACCTGAAGGTAGTGGAATTATTGC
TCCAGCATAAGGCATTGGTGAACACCACCGGGTATCAAAATGACTCACCA
CTTCACGATGCAGCCAAGAATGGGCACGTGGATATAGTCAAGCTGTTACT
TTCCTATGGAGCCTCCAGAAATGCTGTTAATATATTTGGTCTGCGGCCTG
TCGATTATACAGATGATGAAAGTATGAAATCGCTATTGCTGCTACCAGAG
AAGAATGAATCATCCTCAGCTAGCCACTGCTCAGTAATGAACACTGGGCA
GCGTAGGGATGGACCTCTTGTACTTATAGGCAGTGGGCTGTCTTCAGAAC
AACAGAAAATGCTCAGTGAGCTTGCAGTAATTCTTAAGGCTAAAAAATAT
ACTGAGTTTGACAGTACAGTAACTCATGTTGTTGTTCCTGGTGATGCAGT
TCAAAGTACCTTGAAGTGTATGCTTGGGATTCTCAATGGATGCTGGATTC
TAAAATTTGAATGGGTAAAAGCATGTCTACGAAGAAAAGTATGTGAACAG
GAAGAAAAGTATGAAATTCCTGAAGGTCCACGCAGAAGCAGGCTCAACAG
AGAACAGCTGTTGCCAAAGCTGTTTGATGGATGCTACTTCTATTTGTGGG
GAACCTTCAAACACCATCCAAAGGACAACCTTATTAAGCTCGTCACTGCA
GGTGGGGGCCAGATCCTCAGTAGAAAGCCCAAGCCAGACAGTGACGTGAC
TCAGACCATCAATACAGTCGCATACCATGCGAGACCCGATTCTGATCAGC
GCTTCTGCACACAGTATATCATCTATGAAGATTTGTGTAATTATCACCCA
GAGAGGGTTCGGCAGGGCAAAGTCTGGAAGGCTCCTTCGAGCTGGTTTAT
AGACTGTGTGATGTCCTTTGAGTTGCTTCCTCTTGACAGCTGAGCGGCCG
C
Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMPDN
RQPRNRQPRIRSGNEPRSAPAMEPDGRGAWAHSRAALDRLEKLLRCSRCT
NILREPVCLGGCEHIFCSNCVSDCIGTGCPVCYTPAWIQDLKINRQLDSM
IQLCSKLRNLLHDNELSDLKEDKPRKSLFNDAGNKKNSIKMWFSPRSKKV
RYVVSKASVQTQPAIKKDASAQQDSYEFVSPSPPADVSERAKKASARSGK
KQKKKTLAEINQKWNLEAEKEDGEFDSKEESKQKLVSFCSQPSVISSPQI
NGEIDLLASGSLTESECFGSLTEVSLPLAEQIESPDTKSRNEVVTPEKVC
KNYLTSKKSLPLENNGKRGHHNRLSSPISKRCRTSILSTSGDFVKQTVPS
ENIPLPECSSPPSCKRKVGGTSGRKNSNMSDEFISLSPGTPPSTLSSSSY
RQVMSSPSAMKLLPNMAVKRNHRGETLLHIASIKGDIPSVEYLLQNGSDP
NVKDHAGWTPLHEACNHGHLKVVELLLQHKALVNTTGYQNDSPLHDAAKN
GHVDIVKLLLSYGASRNAVNIFGLRPVDYTDDESMKSLLLLPEKNESSSA
SHCSVMNTGQRRDGPLVLIGSGLSSEQQKMLSELAVILKAKKYTEFDSTV
THVVVPGDAVQSTLKCMLGILNGCWILKFEWVKACLRRKVCEQEEKYEIP
EGPRRSRLNREQLLPKLFDGCYFYLWGTFKHHPKDNLIKLVTAGGGQILS
RKPKPDSDVTQTINTVAYHARPDSDQRFCTQYIIYEDLCNYHPERVRQGK
VWKAPSSWFIDCVMSFELLPLDS*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter GST for mammalian expression.

Estimated MW of expressed protein : 114822 Daltons
100042 R 1000 1001 1047 19007
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