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Plasmid
pCMV5 STREP P 6His Ubiquitin K48R K63R
Parent Plasmid
pCMV5 strep 3C 6His
Expressed
STREP P 6His Ubiquitin K48R K63R
DU Number
DU3754
Genbank
P62988
Tag
6His
Species
Human
Sequence of Insert
View Sequence
GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCAGATCTT
CGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTG
ACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCT
CCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACG
CACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGGTCC
TCCGTCTCAGAGGTGGGTGATAAGGATCC
CGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTG
ACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCT
CCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACG
CACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGGTCC
TCCGTCTCAGAGGTGGGTGATAAGGATCC
Amino Acid Sequence
View Sequence
MASSLRQILDSQKMEWRSNAGGSELALEVLFQGPWHHHHHHGSATMYPYD
VPDYAQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
GRQLEDGRTLSDYNIQRESTLHLVLRLRGG*
VPDYAQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
GRQLEDGRTLSDYNIQRESTLHLVLRLRGG*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N terminal STREP/6His/HA tag for mammalian expression. (Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7480-5)
100042 R 1000 1001 1047 19007
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