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Plasmid
pGEX6P-1-Ubiquitin K27wt
Parent Plasmid
pGEX6P1
Expressed
GST-Ubiquitin K27wt
DU Number
DU3563
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCTCAAATCTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACA
GGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG
AGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACA
GGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIFVRTLTGRTITLEVEPS
DTIENVRAKIQDREGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLV
LRLRGG
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIFVRTLTGRTITLEVEPS
DTIENVRAKIQDREGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLV
LRLRGG
Vector Type
Bacterial
Antibiotic
Amp
Comments
N terminal GST for bacterial expression.
100042 R 1000 1001 1047 19007
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