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Plasmid
pCMV5 STREP P 6His HA Ubiquitin K48R
Parent Plasmid
pCMV5 strep 3C 6His
Expressed
STREP P 6His HA Ubiquitin K48R
DU Number
DU3677
Genbank
P62988
Tag
6His
Species
Human
Sequence of Insert
View Sequence
GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCAGATCTT
CGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTG
ACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCT
CCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACG
CACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCC
TCCGTCTCAGAGGTGGGTGATAAGGATCC
CGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTG
ACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCT
CCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACG
CACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCC
TCCGTCTCAGAGGTGGGTGATAAGGATCC
Amino Acid Sequence
View Sequence
MASSLRQILDSQKMEWRSNAGGSELALEVLFQGPWHHHHHHGSATMYPYD
VPDYAQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
GRQLEDGRTLSDYNIQKESTLHLVLRLRGG*
VPDYAQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
GRQLEDGRTLSDYNIQKESTLHLVLRLRGG*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N terminal STREP/6His/HA tag for mammalian expression. (Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7480-5)
100042 R 1000 1001 1047 19007
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