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Plasmid
pET43 DU FAN1 C.elegans
Parent Plasmid
pET43a +3C
Expressed
NUS-6xHis-S.TAG-Prec-FAN1 C.elegans
DU Number
DU33675
Genbank
NM_068596.3
Tag
6His
Species
Caenorhabditis elegans
Sequence of Insert
View Sequence
GGATCCATGAAGAAAGCCAAGAAAGAGAAGAAAGAAAAGATCATTAAGCCAGACGGACCCATTAACTTTAATCGGAGTATCATGGCCGCTTTCGAGAAACAGAGCCGGGGCAAGATTTGCCCTCTGTGCGAAACTAAATTTTCACTGGCCAGCTACAAGTCCCACATGAACACCTGCAATGTGGCTGACGATGACGAGGAAATCGAAGTGATTGCCACATATACTCGCGACGAGGCTATCCTGATGAGGGCTGGACCAGAAATCATTCTGGGGGATGCCTCCTTCTCTGACAAGAGCGAGAACCCCACCAAACGGAGAAAGACAGATGAGCGGGAAGTGCCATCAGAGGATGACATTGTGCCCGAAGTGCCCGGACCTAGCGGCATCGTGAAGAACCACGAGATGCCTAGTGAATCACTGGACGTGACCGAGATCAGCGAAAATATTGAGAAAGTGATCAAGAAGAGCCCAGAGTGGATCAACCATCGGCGGCGGAGCAGCCGGCTGCTCCAGAACAGCCAGAAAGACCAGGCCGATAACGCTAATAAGGAGGACCCAGTGAAGAAAGAAACCGCCACAATTTCTGAGGTGCTCCAGGCTATCGAACGCTTCGAGCAGCGGGTGAGTGGACCAGAGCAGACATGGCCTTACTACATCAAAATCACTATCAAAATCATGAAGAGAGTGATCAGCACTGAGAAGTTCGACGGGACCTTTTACGCCGATGACTTTTGGCTGCCCTCCGATATCATTACATTCTATCGCTTTGTGGAGCTGCTGTCTGAAGGAGCTAAATGTCTGCTGGTGAGACTGTTCATCAGGAAGCCTGCCTGGTACAACCTGGAAAAACTGGAGCAGAAGTATCCTGAGATCCCAAATATCAAGGAGGCCGTGTCCGAACTGGCTAAGGGACACTTCATCGATGACGATTCTAGTATGAAAACCCTGGATGAGGCCCTCCAGATCAGCGACGTGGTGGCTCTGAAGAACGTGACCAAGAAATTCAAACTGGATGGCACAAAGAATCGCCAGGAGCTGATCCAGTCCCTGCGGAAATTTGCCCAGTCTCAGCAGAGTATTTTCGGCGGGACCGGCAACGTGGAGAAGAGCATCCTGAAATCCCTGAAGCAGGAACTGGGACCATGCGTGAGAGTGAGGGGAGGATTCGTGGACCTGTTTAAGTGCCTGTTCACAATCTACTGTCCCGTGACCACAAACTCAGCCAATGTGATCGACAACCCTAGCACTACCAACGTGTACCAGGATCTGCTGTATCTGATGCTGTCCGTGGCCAACGGGACAGTGCAGTTTCCAGCTCCCAATCCTTGTCCAATCATTGCCTCTTTCTACAAAAACAGGAATATGCTCCAGGATTATATGATCAGCAAGTCCCTGGAGATCGCCATCGTGAGCCAGATGAGCAACGGAAATCTGGACGCCGCTCTGGATCTGGCCATTGACGCTAAAGAGTTCATCGAGCAGATGAGCGACGATGACAAGAGATACTATGAATCCCTGGAGATTCACGAAAGGAAGTTTACAAGCATCTGGGTGTTCACTCGCTGCTGTGGGCATGCTTCAAGCATCCTGGAGCGGCAGAAGAAATACGGAATGGCCGTGGAATGGCAGAAGGACCTGCTGATTACCAACAAGGACATCCAGTCTTATTGCATTGATAGTCGCGGCATCTGGTGGGACCGGATGCTGCTGAATCTGGATAGTCACCTGAAAGAGAAGAAAGAATGTGCCAAGATGATCCAGATCGCCCTCCAGGACCCCTCTATTCTGGAGAAGGAACTGCTGATGATCCAGGACAGGGCTCTGAAGCTGAAAGAGATGCCAGCCGATTTCGTGACACCCATCAACATTGGGAATCCTGAAAAGAAAACTATTACCGCCAACGTGATCACTAAGAGCCTGGGGGACGGACGCATTAATCGGTTTATGATCCGCGATCACGAAACTGATGACGATGTGGAGTGCAGCGTGGAGGAAGTGACCAGAAGGCATTACCTGGAGAACGAAGGGTTCTCCACAGGAGTGCACGATGAGGGATCTACATGGCATACTCTGTTTGGCCTGTTCTTTTATGATGTGATCTTCGCCACTGACGAGTCCGTGGAATCTACCTGGCTGTCAGAGCTGCAAGATTGCCCAAGTGACCTGTCAAACACTCTGTACAGCAAGAGAAAAGAGAAGTTTGAAGACAGGTTCGTGTGGCTGGAGGAAGCTGAGCAGGAACTGATCGAGGAAAACATCAGGAAAATTTGGGACCTGAAGCACAACGAGACCAATCGCGAATGTAGTTGGAAGCAGTTCCCTATGGGCGCCGAGGATTGCGTGTCATTCTTTCAGTGTATCCCCCGGCCTGCTCTGATCCTGATTCTGCGCCGGCTGGCCGAGAACTATAGAAATTCTAGGAGTGGGTTTCCTGACCTGACCCTGTGGAACCCAGAAACAAAAAGAGTGGCTGTGGTGGAGGTGAAGGGACCAGGGGACAGACTGAGCACCAAGCAGAGGCTGTGGCTGGCCATTTTCGCTGATTCCGGCATCCGGGCTGAGGTGTGCCATGTGGCTGCTCAGAACAGCCGGCTGCTGGTGTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEATSGSGHHHHHHSAGKETAAAKFERQHMDSPPPTGLVPRGSAGSGTIDDDDKSPGARGSMKKAKKEKKEKIIKPDGPINFNRSIMAAFEKQSRGKICPLCETKFSLASYKSHMNTCNVADDDEEIEVIATYTRDEAILMRAGPEIILGDASFSDKSENPTKRRKTDEREVPSEDDIVPEVPGPSGIVKNHEMPSESLDVTEISENIEKVIKKSPEWINHRRRSSRLLQNSQKDQADNANKEDPVKKETATISEVLQAIERFEQRVSGPEQTWPYYIKITIKIMKRVISTEKFDGTFYADDFWLPSDIITFYRFVELLSEGAKCLLVRLFIRKPAWYNLEKLEQKYPEIPNIKEAVSELAKGHFIDDDSSMKTLDEALQISDVVALKNVTKKFKLDGTKNRQELIQSLRKFAQSQQSIFGGTGNVEKSILKSLKQELGPCVRVRGGFVDLFKCLFTIYCPVTTNSANVIDNPSTTNVYQDLLYLMLSVANGTVQFPAPNPCPIIASFYKNRNMLQDYMISKSLEIAIVSQMSNGNLDAALDLAIDAKEFIEQMSDDDKRYYESLEIHERKFTSIWVFTRCCGHASSILERQKKYGMAVEWQKDLLITNKDIQSYCIDSRGIWWDRMLLNLDSHLKEKKECAKMIQIALQDPSILEKELLMIQDRALKLKEMPADFVTPINIGNPEKKTITANVITKSLGDGRINRFMIRDHETDDDVECSVEEVTRRHYLENEGFSTGVHDEGSTWHTLFGLFFYDVIFATDESVESTWLSELQDCPSDLSNTLYSKRKEKFEDRFVWLEEAEQELIEENIRKIWDLKHNETNRECSWKQFPMGAEDCVSFFQCIPRPALILILRRLAENYRNSRSGFPDLTLWNPETKRVAVVEVKGPGDRLSTKQRLWLAIFADSGIRAEVCHVAAQNSRLLV*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Estimated MW of expressed protein : 160165.04 Daltons
All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.