FAN1 (C.elegans)

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Plasmid
pcDNA5-FRT/TO-GFP FAN1 C.elegans
Parent Plasmid
pcDNA5 FRT/TO GFP
Expressed
GFP-FAN1 C.elegans
DU Number
DU19995
Genbank
NM_068596.3
Tag
GFP
Species
Caenorhabditis elegans
Sequence of Insert
GGATCCATGAAGAAAGCCAAGAAAGAGAAGAAAGAAAAGATCATTAAGCCAGACGGACCCATTAACTTTAATCGGAGTATCATGGCCGCTTTCGAGAAACAGAGCCGGGGCAAGATTTGCCCTCTGTGCGAAACTAAATTTTCACTGGCCAGCTACAAGTCCCACATGAACACCTGCAATGTGGCTGACGATGACGAGGAAATCGAAGTGATTGCCACATATACTCGCGACGAGGCTATCCTGATGAGGGCTGGACCAGAAATCATTCTGGGGGATGCCTCCTTCTCTGACAAGAGCGAGAACCCCACCAAACGGAGAAAGACAGATGAGCGGGAAGTGCCATCAGAGGATGACATTGTGCCCGAAGTGCCCGGACCTAGCGGCATCGTGAAGAACCACGAGATGCCTAGTGAATCACTGGACGTGACCGAGATCAGCGAAAATATTGAGAAAGTGATCAAGAAGAGCCCAGAGTGGATCAACCATCGGCGGCGGAGCAGCCGGCTGCTCCAGAACAGCCAGAAAGACCAGGCCGATAACGCTAATAAGGAGGACCCAGTGAAGAAAGAAACCGCCACAATTTCTGAGGTGCTCCAGGCTATCGAACGCTTCGAGCAGCGGGTGAGTGGACCAGAGCAGACATGGCCTTACTACATCAAAATCACTATCAAAATCATGAAGAGAGTGATCAGCACTGAGAAGTTCGACGGGACCTTTTACGCCGATGACTTTTGGCTGCCCTCCGATATCATTACATTCTATCGCTTTGTGGAGCTGCTGTCTGAAGGAGCTAAATGTCTGCTGGTGAGACTGTTCATCAGGAAGCCTGCCTGGTACAACCTGGAAAAACTGGAGCAGAAGTATCCTGAGATCCCAAATATCAAGGAGGCCGTGTCCGAACTGGCTAAGGGACACTTCATCGATGACGATTCTAGTATGAAAACCCTGGATGAGGCCCTCCAGATCAGCGACGTGGTGGCTCTGAAGAACGTGACCAAGAAATTCAAACTGGATGGCACAAAGAATCGCCAGGAGCTGATCCAGTCCCTGCGGAAATTTGCCCAGTCTCAGCAGAGTATTTTCGGCGGGACCGGCAACGTGGAGAAGAGCATCCTGAAATCCCTGAAGCAGGAACTGGGACCATGCGTGAGAGTGAGGGGAGGATTCGTGGACCTGTTTAAGTGCCTGTTCACAATCTACTGTCCCGTGACCACAAACTCAGCCAATGTGATCGACAACCCTAGCACTACCAACGTGTACCAGGATCTGCTGTATCTGATGCTGTCCGTGGCCAACGGGACAGTGCAGTTTCCAGCTCCCAATCCTTGTCCAATCATTGCCTCTTTCTACAAAAACAGGAATATGCTCCAGGATTATATGATCAGCAAGTCCCTGGAGATCGCCATCGTGAGCCAGATGAGCAACGGAAATCTGGACGCCGCTCTGGATCTGGCCATTGACGCTAAAGAGTTCATCGAGCAGATGAGCGACGATGACAAGAGATACTATGAATCCCTGGAGATTCACGAAAGGAAGTTTACAAGCATCTGGGTGTTCACTCGCTGCTGTGGGCATGCTTCAAGCATCCTGGAGCGGCAGAAGAAATACGGAATGGCCGTGGAATGGCAGAAGGACCTGCTGATTACCAACAAGGACATCCAGTCTTATTGCATTGATAGTCGCGGCATCTGGTGGGACCGGATGCTGCTGAATCTGGATAGTCACCTGAAAGAGAAGAAAGAATGTGCCAAGATGATCCAGATCGCCCTCCAGGACCCCTCTATTCTGGAGAAGGAACTGCTGATGATCCAGGACAGGGCTCTGAAGCTGAAAGAGATGCCAGCCGATTTCGTGACACCCATCAACATTGGGAATCCTGAAAAGAAAACTATTACCGCCAACGTGATCACTAAGAGCCTGGGGGACGGACGCATTAATCGGTTTATGATCCGCGATCACGAAACTGATGACGATGTGGAGTGCAGCGTGGAGGAAGTGACCAGAAGGCATTACCTGGAGAACGAAGGGTTCTCCACAGGAGTGCACGATGAGGGATCTACATGGCATACTCTGTTTGGCCTGTTCTTTTATGATGTGATCTTCGCCACTGACGAGTCCGTGGAATCTACCTGGCTGTCAGAGCTGCAAGATTGCCCAAGTGACCTGTCAAACACTCTGTACAGCAAGAGAAAAGAGAAGTTTGAAGACAGGTTCGTGTGGCTGGAGGAAGCTGAGCAGGAACTGATCGAGGAAAACATCAGGAAAATTTGGGACCTGAAGCACAACGAGACCAATCGCGAATGTAGTTGGAAGCAGTTCCCTATGGGCGCCGAGGATTGCGTGTCATTCTTTCAGTGTATCCCCCGGCCTGCTCTGATCCTGATTCTGCGCCGGCTGGCCGAGAACTATAGAAATTCTAGGAGTGGGTTTCCTGACCTGACCCTGTGGAACCCAGAAACAAAAAGAGTGGCTGTGGTGGAGGTGAAGGGACCAGGGGACAGACTGAGCACCAAGCAGAGGCTGTGGCTGGCCATTTTCGCTGATTCCGGCATCCGGGCTGAGGTGTGCCATGTGGCTGCTCAGAACAGCCGGCTGCTGGTGTGAGCGGCCGC
Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMKKAKKEKKEKIIKPDGPINFNRSIMAAFEKQSRGKICPLCETKFSLASYKSHMNTCNVADDDEEIEVIATYTRDEAILMRAGPEIILGDASFSDKSENPTKRRKTDEREVPSEDDIVPEVPGPSGIVKNHEMPSESLDVTEISENIEKVIKKSPEWINHRRRSSRLLQNSQKDQADNANKEDPVKKETATISEVLQAIERFEQRVSGPEQTWPYYIKITIKIMKRVISTEKFDGTFYADDFWLPSDIITFYRFVELLSEGAKCLLVRLFIRKPAWYNLEKLEQKYPEIPNIKEAVSELAKGHFIDDDSSMKTLDEALQISDVVALKNVTKKFKLDGTKNRQELIQSLRKFAQSQQSIFGGTGNVEKSILKSLKQELGPCVRVRGGFVDLFKCLFTIYCPVTTNSANVIDNPSTTNVYQDLLYLMLSVANGTVQFPAPNPCPIIASFYKNRNMLQDYMISKSLEIAIVSQMSNGNLDAALDLAIDAKEFIEQMSDDDKRYYESLEIHERKFTSIWVFTRCCGHASSILERQKKYGMAVEWQKDLLITNKDIQSYCIDSRGIWWDRMLLNLDSHLKEKKECAKMIQIALQDPSILEKELLMIQDRALKLKEMPADFVTPINIGNPEKKTITANVITKSLGDGRINRFMIRDHETDDDVECSVEEVTRRHYLENEGFSTGVHDEGSTWHTLFGLFFYDVIFATDESVESTWLSELQDCPSDLSNTLYSKRKEKFEDRFVWLEEAEQELIEENIRKIWDLKHNETNRECSWKQFPMGAEDCVSFFQCIPRPALILILRRLAENYRNSRSGFPDLTLWNPETKRVAVVEVKGPGDRLSTKQRLWLAIFADSGIRAEVCHVAAQNSRLLV*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Mammalian expression of protein with N-ter GFP tag.

Estimated MW of expressed protein : 126731.71 Daltons

All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.
100042 R 1000 1001 1047 19007
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