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Plasmid
pGEX6P-1-ATXN3 F74A
Parent Plasmid
pGEX6P1
Expressed
GST ATXN3 F74A
DU Number
DU65487
Genbank
AAH33711.1
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGAGTCCATCTTCCACGAGAAACAAGAAGGCTCACTTTGTGC
TCAACATTGCCTGAATAACTTATTGCAAGGAGAATATTTTAGCCCTGTGG
AATTATCCTCAATTGCACATCAGCTGGATGAGGAGGAGAGGATGAGAATG
GCAGAAGGAGGAGTTACTAGTGAAGATTATCGCACGTTTTTACAGCAGCC
TTCTGGAAATATGGATGACAGTGGTgcTTTCTCTATTCAGGTTATAAGCA
ATGCCTTGAAAGTTTGGGGTTTAGAACTAATCCTGTTCAACAGTCCAGAG
TATCAGAGGCTCAGGATCGATCCTATAAATGAAAGATCATTTATATGCAA
TTATAAGGAACACTGGTTTACAGTTAGAAAATTAGGAAAACAGTGGTTTA
ACTTGAATTCTCTCTTGACGGGTCCAGAATTAATATCAGATACATATCTT
GCACTTTTCTTGGCTCAATTACAACAGGAAGGTTATTCTATATTTGTTGT
TAAGGGTGATCTGCCAGATTGCGAAGCTGACCAACTCCTGCAGATGATTA
GGGTCCAACAGATGCATCGACCAAAACTTATTGGAGAAGAATTAGCACAA
CTAAAAGAGCAAAGAGTCCATAAAACAGACCTGGAACGAGTGTTAGAAGC
AAATGATGGCTCAGGAATGTTAGACGAAGATGAGGAGGATTTGCAGAGGG
CTCTGGCACTAAGTCGCCAAGAAATTGACATGGAAGATGAGGAAGCAGAT
CTCCGCAGGGCTATTCAGCTAAGTATGCAAGGTAGTTCCAGAAACATATC
TCAAGATATGACACAGACATCAGGTACAAATCTTACTTCAGAAGAGCTTC
GGAAGAGACGAGAAGCCTACTTTGAAAAACAGCAGCAAAAGCAGCAACAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGGG
GGACCTATCAGGACAGAGTTCACATCCATGTGAAAGGCCAGCCACCAGTT
CAGGAGCACTTGGGAGTGATCTAGGTGATGCTATGAGTGAAGAAGACATG
CTTCAGGCAGCTGTGACCATGTCTTTAGAAACTGTCAGAAATGATTTGAA
AACAGAAGGAAAAAAATAAGCGGCCGC
TCAACATTGCCTGAATAACTTATTGCAAGGAGAATATTTTAGCCCTGTGG
AATTATCCTCAATTGCACATCAGCTGGATGAGGAGGAGAGGATGAGAATG
GCAGAAGGAGGAGTTACTAGTGAAGATTATCGCACGTTTTTACAGCAGCC
TTCTGGAAATATGGATGACAGTGGTgcTTTCTCTATTCAGGTTATAAGCA
ATGCCTTGAAAGTTTGGGGTTTAGAACTAATCCTGTTCAACAGTCCAGAG
TATCAGAGGCTCAGGATCGATCCTATAAATGAAAGATCATTTATATGCAA
TTATAAGGAACACTGGTTTACAGTTAGAAAATTAGGAAAACAGTGGTTTA
ACTTGAATTCTCTCTTGACGGGTCCAGAATTAATATCAGATACATATCTT
GCACTTTTCTTGGCTCAATTACAACAGGAAGGTTATTCTATATTTGTTGT
TAAGGGTGATCTGCCAGATTGCGAAGCTGACCAACTCCTGCAGATGATTA
GGGTCCAACAGATGCATCGACCAAAACTTATTGGAGAAGAATTAGCACAA
CTAAAAGAGCAAAGAGTCCATAAAACAGACCTGGAACGAGTGTTAGAAGC
AAATGATGGCTCAGGAATGTTAGACGAAGATGAGGAGGATTTGCAGAGGG
CTCTGGCACTAAGTCGCCAAGAAATTGACATGGAAGATGAGGAAGCAGAT
CTCCGCAGGGCTATTCAGCTAAGTATGCAAGGTAGTTCCAGAAACATATC
TCAAGATATGACACAGACATCAGGTACAAATCTTACTTCAGAAGAGCTTC
GGAAGAGACGAGAAGCCTACTTTGAAAAACAGCAGCAAAAGCAGCAACAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGGG
GGACCTATCAGGACAGAGTTCACATCCATGTGAAAGGCCAGCCACCAGTT
CAGGAGCACTTGGGAGTGATCTAGGTGATGCTATGAGTGAAGAAGACATG
CTTCAGGCAGCTGTGACCATGTCTTTAGAAACTGTCAGAAATGATTTGAA
AACAGAAGGAAAAAAATAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMESIFHEKQEGSLCAQHCL
NNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNM
DDSGAFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEH
WFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDL
PDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGS
GMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMT
QTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQQQQQQQQQQGDLSG
QSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGK
K*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMESIFHEKQEGSLCAQHCL
NNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNM
DDSGAFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEH
WFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDL
PDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGS
GMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMT
QTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQQQQQQQQQQGDLSG
QSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGK
K*
Vector Type
Bacterial
Antibiotic
Amp
Comments
silent change at Val164 (GTC > GTT)compared to NCBI ref
100042 R 1000 1001 1047 19007
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