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Plasmid
pGEX6P-1-FLAG-2Gly-Ubiquitin KallR
Parent Plasmid
pGEX6P-1
Expressed
GST-RV3C-FLAG-Gly-Gly-Ubiquitin [K all R]
DU Number
DU29879
Genbank
P62987
Tag
GST
FLAG
Species
Human
Sequence of Insert
View Sequence
GAATTCGCCCTTGCAGATCTGCCACCATGGACTACAAGGACGACGATGAC
AAGGGAGGCATGCAAATCTTCGTGAGGACCCTGACTGGTAGGACCATCAC
TCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAGGATCC
AAGACAGGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGG
AGACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGA
GTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG
AAGGGAGGCATGCAAATCTTCGTGAGGACCCTGACTGGTAGGACCATCAC
TCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAGGATCC
AAGACAGGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGG
AGACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGA
GTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSRIRPCRSATMDYKDDDDKG
GMQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQ
LEDGRTLSDYNIQRESTLHLVLRLRGG**
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSRIRPCRSATMDYKDDDDKG
GMQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQ
LEDGRTLSDYNIQRESTLHLVLRLRGG**
Vector Type
Bacterial
Antibiotic
Amp
Comments
Gly-Gly spacer inserted and ubiquitin native methionine restored
100042 R 1000 1001 1047 19007
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