ISG15 | Ubiquitylation Resource

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Plasmid

pTXB1-ISG15 (aa1-154) C78S T3C Mxe intein CBD

Parent Plasmid

pTXB1

Expressed

ISG15 (aa1-154) C78S T3C Mxe intein CBD

DU Number

DU29486

Genbank

NM_005101.4

Tag

Intein

Species

Human

Sequence of Insert

View Sequence

GCTAGCATGGGTTGCTCTTCCGGCGGCTGGGACCTGACGGTGAAGATGCT
GGCGGGCAACGAATTCCAGGTGTCCCTGAGCAGCTCCATGTCGGTGTCAG
AGCTGAAGGCGCAGATCACCCAGAAGATCGGCGTGCACGCCTTCCAGCAG
CGTCTGGCTGTCCACCCGAGCGGTGTGGCGCTGCAGGACAGGGTCCCCCT
TGCCAGCCAGGGCCTGGGCCCCGGCAGCACGGTCCTGCTGGTGGTGGACA
AATCCGACGAACCTCTGAACATCCTGGTGAGGAATAACAAGGGCCGCAGC
AGCACCTACGAGGTGCGGCTGACGCAGACCGTGGCCCACCTGAAGCAGCA
AGTGAGCGGGCTGGAGGGTGTGCAGGACGACCTGTTCTGGCTGACCTTCG
AGGGGAAGCCCCTGGAGGACCAGCTCCCGCTGGGGGAGTACGGCCTCAAG
CCCCTGAGCACCGTGTTCATGAATCTGCGCCTGTGCATCTGCGGAGATGC
ACTAGT

Amino Acid Sequence

View Sequence

MGCSSGGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRL
AVHPSGVALQDRVPLASQGLGPGSTVLLVVDKSDEPLNILVRNNKGRSST
YEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPL
STVFMNLRLCICGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRH
GNPVLADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLW
KLIDEIKPGDYAVIQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLE
AHHRDPDAQAIADELTDGRFYYAKVASVTDAGVQPVYSLRVDTADHAFIT
NGFVSHATGLTGLNSGLTTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQP
HTSLAGWEPSNVPALWQLQ*

Vector Type

Bacterial

Antibiotic

Amp

Comments

additional N-terminal residues (MAS) derived from translation initiation at the pTXB1 vector derived Nde1 site. Native ISG15 Met1 = Met4 of translation product (with GCSSG inserted between native Met1 and Gly2)
Intein T3C mutant
Carries known SNP S83N and silent change at Val98 (GTA>GTG) compared to NCBI ref NM_005101.4
PARENTAL 45876

100042 R 1000 1001 1047 19007

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