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Plasmid
pFastbac 6HIS TEV GST 3C AVI IRAK2 192-625
Parent Plasmid
pFastbac
Expressed
6HIS TEV GST 3C AVI IRAK2 192-625
DU Number
DU56369
Tag
GST
6His
AVI
Species
Human
Sequence of Insert
View Sequence
GGCCCGGCCTGAACGATATTTTTGAAGCGCAGAAAAGCGGCGGCAGCGGCCTGGGATCCGCTGGAGACAGCCTTTTCTGGAGTGAGGCAGACGTGGTCCA
GGCAACCGATGACTTCAATCAAAACCGCAAAATCAGCCAGGGGACCTTTGCTGACGTCTACAGAGGGCACAGGCACGGGAAGCCATTCGTCTTCAAGAAG
CTCAGAGAGACAGCCTGTTCAAGTCCAGGATCAATCGAAAGATTCTTCCAGGCAGAGTTGCAGATTTGTCTTAGATGCTGCCACCCCAATGTCTTACCTG
TGCTGGGCTTCTGTGCTGCAAGACAGTTTCACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTGCAGGGTCAGGGTGGCTCGGA
CCCCCTCCCCTGGCCCCAGCGTGTCAGCATCTGCTCAGGGCTGCTCTGTGCCGTCGAGTACCTGCATGGTCTGGAGATCATCCACAGCAACGTCAAGAGC
TCTAATGTCTTGCTGGACCAAAATCTCACCCCCAAACTTGCTCACCCAATGGCTCATCTGTGTCCTGTCAACAAAAGGTCAAAATACACCATGATGAAGA
CTCACCTGCTCCGGACGTCAGCCGCGTATCTGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGACATCTTCAGCTGTGGAATAGTGTT
GGCCGAGGTCCTCACGGGCATCCCTGCAATGGATAACAACCGAAGCCCGGTTTACCTGAAGGACTTACTCCTCAGTGATATTCCAAGCAGCACCGCCTCG
CTCTGCTCCAGGAAGACGGGCGTGGAGAACGTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAGAAGGGCGCAGGGAGGCTTCCGGAGGACTGCGCCG
AGGCCCTGGCCACGGCTGCCTGCCTGTGCCTGCGGAGGCGTAACACCAGCCTGCAGGAGGTGTGTGGCTCTGTGGCTGCTGTGGAAGAGCGGCTCCGAGG
TCGGGAGACGTTGCTCCCTTGGAGTGGGCTTTCTGAGGGTACAGGCTCTTCTTCCAACACCCCAGAGGAAACAGACGACGTTGACAATTCCAGCCTTGAT
GCCTCCTCCTCCATGAGTGTGGCACCCTGGGCAGGGGCTGCCACCCCACTTCTCCCCACAGAGAATGGGGAAGGAAGGCTGCGGGTCATCGTGGGAAGGG
AGGCTGACTCCTCCTCTGAGGCCTGTGTTGGCCTGGAGCCTCCCCAGGATGTTACAGAAACTTCGTGGCAAATTGAGATCAATGAGGCCAAAAGGAAACT
GATGGAGAATATTCTGCTCTACAAAGAGGAAAAAGTGGACAGCATTGAGCTCTTTGGCCCCTAAgcggccgc
GGCAACCGATGACTTCAATCAAAACCGCAAAATCAGCCAGGGGACCTTTGCTGACGTCTACAGAGGGCACAGGCACGGGAAGCCATTCGTCTTCAAGAAG
CTCAGAGAGACAGCCTGTTCAAGTCCAGGATCAATCGAAAGATTCTTCCAGGCAGAGTTGCAGATTTGTCTTAGATGCTGCCACCCCAATGTCTTACCTG
TGCTGGGCTTCTGTGCTGCAAGACAGTTTCACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTGCAGGGTCAGGGTGGCTCGGA
CCCCCTCCCCTGGCCCCAGCGTGTCAGCATCTGCTCAGGGCTGCTCTGTGCCGTCGAGTACCTGCATGGTCTGGAGATCATCCACAGCAACGTCAAGAGC
TCTAATGTCTTGCTGGACCAAAATCTCACCCCCAAACTTGCTCACCCAATGGCTCATCTGTGTCCTGTCAACAAAAGGTCAAAATACACCATGATGAAGA
CTCACCTGCTCCGGACGTCAGCCGCGTATCTGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGACATCTTCAGCTGTGGAATAGTGTT
GGCCGAGGTCCTCACGGGCATCCCTGCAATGGATAACAACCGAAGCCCGGTTTACCTGAAGGACTTACTCCTCAGTGATATTCCAAGCAGCACCGCCTCG
CTCTGCTCCAGGAAGACGGGCGTGGAGAACGTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAGAAGGGCGCAGGGAGGCTTCCGGAGGACTGCGCCG
AGGCCCTGGCCACGGCTGCCTGCCTGTGCCTGCGGAGGCGTAACACCAGCCTGCAGGAGGTGTGTGGCTCTGTGGCTGCTGTGGAAGAGCGGCTCCGAGG
TCGGGAGACGTTGCTCCCTTGGAGTGGGCTTTCTGAGGGTACAGGCTCTTCTTCCAACACCCCAGAGGAAACAGACGACGTTGACAATTCCAGCCTTGAT
GCCTCCTCCTCCATGAGTGTGGCACCCTGGGCAGGGGCTGCCACCCCACTTCTCCCCACAGAGAATGGGGAAGGAAGGCTGCGGGTCATCGTGGGAAGGG
AGGCTGACTCCTCCTCTGAGGCCTGTGTTGGCCTGGAGCCTCCCCAGGATGTTACAGAAACTTCGTGGCAAATTGAGATCAATGAGGCCAAAAGGAAACT
GATGGAGAATATTCTGCTCTACAAAGAGGAAAAAGTGGACAGCATTGAGCTCTTTGGCCCCTAAgcggccgc
Amino Acid Sequence
View Sequence
MHHHHHHENLYFQGMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCP
KERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAI
PQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPGLNDIFEAQKSGGSGLGSAGDSLFWSEADVVQATDDFNQNRKISQGTFADVYRGHRHG
KPFVFKKLRETACSSPGSIERFFQAELQICLRCCHPNVLPVLGFCAARQFHSFIYPYMANGSLQDRLQGQGGSDPLPWPQRVSICSGLLCAVEYLHGLEI
IHSNVKSSNVLLDQNLTPKLAHPMAHLCPVNKRSKYTMMKTHLLRTSAAYLPEDFIRVGQLTKRVDIFSCGIVLAEVLTGIPAMDNNRSPVYLKDLLLSD
IPSSTASLCSRKTGVENVMAKEICQKYLEKGAGRLPEDCAEALATAACLCLRRRNTSLQEVCGSVAAVEERLRGRETLLPWSGLSEGTGSSSNTPEETDD
VDNSSLDASSSMSVAPWAGAATPLLPTENGEGRLRVIVGREADSSSEACVGLEPPQDVTETSWQIEINEAKRKLMENILLYKEEKVDSIELFGP*
KERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAI
PQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPGLNDIFEAQKSGGSGLGSAGDSLFWSEADVVQATDDFNQNRKISQGTFADVYRGHRHG
KPFVFKKLRETACSSPGSIERFFQAELQICLRCCHPNVLPVLGFCAARQFHSFIYPYMANGSLQDRLQGQGGSDPLPWPQRVSICSGLLCAVEYLHGLEI
IHSNVKSSNVLLDQNLTPKLAHPMAHLCPVNKRSKYTMMKTHLLRTSAAYLPEDFIRVGQLTKRVDIFSCGIVLAEVLTGIPAMDNNRSPVYLKDLLLSD
IPSSTASLCSRKTGVENVMAKEICQKYLEKGAGRLPEDCAEALATAACLCLRRRNTSLQEVCGSVAAVEERLRGRETLLPWSGLSEGTGSSSNTPEETDD
VDNSSLDASSSMSVAPWAGAATPLLPTENGEGRLRVIVGREADSSSEACVGLEPPQDVTETSWQIEINEAKRKLMENILLYKEEKVDSIELFGP*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
Please insert Avitag sequence (GLNDIFEAQKIEWHE) between 3C cleavage site and protein with 6 aa spacer (e.g. GSGGSG). DU56039 might be suitable template.
100042 R 1000 1001 1047 19007
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