SUMO-2

subcloned into pHISTEV30a vector using EcoRV and HindIII restriction sites
note: EcoRV cleaves the pHISTEV30a plasmid twice, once before the coding
sequence for the TEV protease cleavage site and once in the polylinker. Digestion of the pHISTEV30a vector with EcoRV thus removes the TEV protease cleavage site. The coding sequence for the TEV protease cleavage site was therefore included in the forward primer.
.after cleavage with TEV protease, the construct corresponds to the mature form of SUMO-2 with the diglycine motif at the C-terminus; the first methionine is mutated to a glycine