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Synonyms
E2-EPF, E2EPF, EPF5
Plasmid
pMA UBE2S Nter Puro
Parent Plasmid
pMA
DU Number
DU48148
Genbank
ENSG00000108106
Species
Human
Sequence of Insert
View Sequence
TCAAACGGTCCAATCAGCGGCCGCGTCGCCTTCCCCGTCCCCCTTCCTCTCTTCTTCCCCTTTTCAGCCCCTCCGTTCTTTGAGAACCCTCCCGACCCCCGCGGGCCCCCATTGGCCGGCCCGCTACCCAGCCCTCTCCGCCACTTCCCTCGCTTCTGACCATAGTTTGCGGGGAAGGGAGCGAGCGCGTCGAAAACCAAGGAACGTGCGCGCTGACGTCACGGTTGAGGCTCGGAGCTGAGGGGCCGCGGAGGGCGTGGCCTGCGGGCGGTTATAAAGAGGCAGTGGTGCGCGCGCGGCCGGCTCAGTGCTGCCGGGCACCGGGGCGGCGGGTTGGTCTACGCTGTGCGCGGCGGACGTCGGAGGCAGCGGGGAGCGGAGCGGGGCCGCCGGGGCCTCTCCAGGGCCGCAGCGGCAGCAGTTGGGCCCCCCGCCCCGGCCGGCGGACCGAAGAACGCAGGAAGGGGGCCGGGGGGACCCGCCCCCGGCCGGCCGCAGCCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGTGCCCGAAGGACCGCGCGACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAGTGAGCACGGGCGATGAGGCCCATGAAGGTGGTCGGGGGTGTCCCTGAGGGAGGAGGTGGAGTCTCAGGGGCCTCCGACGGGCGGCCTTGAGGGGTTTGAGGGCCAGCCTGGGGGTCCCTGACCCGGGATGGGCCCGAGGGCCTTCCCGCAGGCGGAGCAGGAGCTCGGGCGGCCCGCAGTCTCCCTGGCGGCGAGGGCTCCGGGCGCTTTGCGGCCCGCCCATGGGTGTTACCAGGAGCAAGGACGCCCCGCCGCCATCTCCAGACCTGAGCCGGGGTTTCAAGGATGCTCTAACCAAAAATTAGTGGAGAAGGGGGAGAGAGTAGATCTCTGGCCTACGTGGAACCACTGGGGACCAAGCAGGGTTAGGAGCGGGCTTTGGGGGGGGGGTTCTGGGGTGGGTGGAGTTATCTGCAAGCCCCCCCGTGAACCCGCCCCCATTTCTGTTGGAAACTCTGCCTGGGCTGATGGCCTGAAGCATCCTGGGTGAGGGTCTGGGcggccgc
Vector Type
Mammalian
Antibiotic
Amp
Comments
Designed to replace the UBE2S coding region of exon 1 with puromycin. Complete sequence of donor including homology arms shown as sequence of insert. Requires modification to add the BGH polyA signl to prevent NMD.
100042 R 1000 1001 1047 19007
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