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Synonyms
E2-EPF, E2EPF, EPF5
Plasmid
pMK-RQ UBE2S Cter GFP
Parent Plasmid
pMK-RQ
DU Number
DU48135
Genbank
ENSG00000108106
Species
Human
Sequence of Insert
View Sequence
gcggccgcTGGCCTCTGAGGGAGACAGGAGCAAAGATGAGGGAGGATGCAGCATAGGTGGGGCCTGGATCTGCAGCTGAGTTGGGAGCAGAAGGAACAGCCAGTGCGGAGGCCACCCTCTTGTGGAGGCCTGGGGGTCCCTGTAGGTTGCCCGACCCACTGTGACCCTGAGCCTCTTCCCACAGACCATCAAGTGCCTGCTGATCCACCCTAACCCCGAGTCTGCACTCAACGAGGAGGCGGGCCGCCTGCTCTTGGAGAACTACGAGGAGTATGCGGCTCGGGCCCGTCTGCTCACAGAGATCCACGGGGGCGCCGGCGGGCCCAGCGGCAGGGCCGAAGCCGGTCGGGCCCTGGCCAGTGGCACTGAAGCTTCCTCCACCGACCCTGGGGCCCCAGGGGGCCCGGGAGGGGCTGAGGGTCCCATGGCCAAGAAGCATGCTGGCGAGCGCGATAAGAAGCTGGCGGCCAAGAAAAAGACGGACAAGAAGCGGGCtCTaCGaCGaCTcGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAGTGGGCTCTCTTCCTCCTTCCACCGTGACCCCAACCTCTCCTGTCCCCTCCCTCCAACTCTGTCTCTAAGTTATTTAAATTATGGCTGGGGTCGGGGAGGGTACAGGGGGCACTGGGACCTGGATTTGTTTTTCTAAATAAAGTTGGAAAAGCAGCTTCTGTGTCTCACAGATGCCCCAGGACCGCTGCATGGTGCCATGTGGCTCTGGGAGTTTGGCTCCCCTGCACAGGCCCTGGGTCCTTGTAAAGCCTCATGGACTGCAGCAGTGGGTCCATCTAGCTCACCAGCCTTGCTGTTTGAGGTTTGGGTCCACCTCTTTGTTCTGTGGAGGTAGAGCGGTCACAACCCCTCTTTGGAGTCAGATTTCCAATCTAAAATGGGGCGAGGACCTGGAGCTGCTCCTGGTGGCCTGAGCGGCACTGCCCTATACTTCTCCCTCTAGAATAATGGCCACGTTGGTGTGGCAGTGGTGTTCCTGCTCAGGACGGCCAGGTGCCAGCggccgc
Amino Acid Sequence
View Sequence
MNSNVENLPPHIIRLVYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGP
EGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHPNVGANGEICVNVLK
RDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARL
LTEIHGGAGGPSGRAEAGRALASGTEASSTDPGAPGGPGGAEGPMAKKHA
GERDKKLAAKKKTDKKRALRRLVSKGEELFTGVVPILVELDGDVNGHKFS
VSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHM
KQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGI
DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQL
ADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAG
ITLGMDELYK*
EGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHPNVGANGEICVNVLK
RDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARL
LTEIHGGAGGPSGRAEAGRALASGTEASSTDPGAPGGPGGAEGPMAKKHA
GERDKKLAAKKKTDKKRALRRLVSKGEELFTGVVPILVELDGDVNGHKFS
VSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHM
KQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGI
DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQL
ADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAG
ITLGMDELYK*
Vector Type
Mammalian
Antibiotic
Kan
Comments
Designed to insert an in-frame GFP upstream of the UBE2S stop codon. Targets last coding exon (exon 4). Complete sequence of donor including homology arms shown as sequence of insert, contains several silent mutations to prevent Cas9 targeting the donor sequence. Sequence of cassette depicts the expressed UBE2S-GFP fusion.
100042 R 1000 1001 1047 19007
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