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HUWE1 UBA-UIM

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Plasmid
pGEX C3 HALO thrombin-HUWE E1308-A1400
Parent Plasmid
pGEX6P Halo Thr
Expressed
GST-C3-HALO-Thr-HUWE E1308-A1400
DU Number
DU24297
Genbank
Uniprot Q7Z6Z7
Tag
GST
HALO
Species
Human
Sequence of Insert
GGATCCGAAGGTGGCTCCCGCCGGGAACCTCAAGTCAACCAGCAACAACTACAACAGCTCATGGACATGGGCTTCACAAGGGAACATGCAATGGAGGCACTGTTGAACACCAGCACCATGGAGCAGGCCACAGAGTACCTTTTAACCCACCCTCCTCCAATCATGGGAGGAGTTGTTCGGGATCTCAGCATGTCTGAAGAGGACCAGATGATGAGAGCAATTGCTATGTCTCTGGGACAGGATATTCCAATGGATCAAAGGGCAGAGTCACCTGAGGAAGTTGCTTAAGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSEGGSRREPQVNQQQ
LQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIMGGVVRDLSMSE
EDQMMRAIAMSLGQDIPMDQRAESPEEVA*
Vector Type
Bacterial
Antibiotic
Amp
Comments
UBA UIM DOMAIN. Silent change compared to NCBI ref at L1322 (CTA instead of CTG). Parental 24192
100042 R 1000 1001 1047 19007
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