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Plasmid
pEGFP-C4P Pellino1
Parent Plasmid
pEGFPC4P1
Expressed
GFP Precission Pellino1
DU Number
DU25235
Genbank
Q96FA3
Tag
GFP
Species
Human
Sequence of Insert
View Sequence
GGATCCATGTTTTCTCCTGATCAAGAAAATCATCCATTTAAAGCACCAGTAAAATATGGTGAACTCATTGTCTTAGGGTATAATGGGTCTCTCCCAAATGGCGATAGAGGAAGGAGGAAAAGTAGGTTTGCTTTGTTTAAAAGACCTAAGGCAAATGGGGTGAAGCCCAGCACTGTGCATATTGCTTGTACTCCTCAGGCTGCAAAGGCAATAAGCAACAAAGACCAGCATAGCATATCATATACTTTGTCTCGGGCCCAGACTGTGGTGGTTGAATATACTCATGACAGCAACACAGATATGTTTCAGATTGGCCGGTCGACTGAAAGCCCCATTGATTTTGTAGTAACTGACACAGTTCCTGGAAGTCAAAGTAATTCTGATACACAGTCAGTACAAAGCACTATATCAAGATTTGCCTGCAGAATCATATGTGAACGGAATCCTCCCTTTACAGCACGGATTTATGCTGCAGGATTTGACTCATCAAAAAACATCTTTCTTGGGGAGAAGGCTGCCAAATGGAAGACATCAGATGGACAGATGGATGGCTTGACCACTAATGGTGTTCTTGTGATGCATCCACGCAATGGGTTCACAGAAGACTCCAAGCCTGGAATATGGAGAGAAATATCGGTGTGTGGAAATGTATTTAGCCTACGTGAAACCAGATCGGCTCAGCAGAGAGGAAAAATGGTGGAAATTGAAACCAATCAGTTACAAGATGGCTCGTTAATTGACCTCTGTGGTGCAACATTGTTATGGCGTACTGCAGAAGGCCTTTCCCACACTCCTACCGTGAAGCATTTAGAGGCTTTAAGACAGGAAATCAATGCAGCACGACCTCAGTGCCCTGTAGGGTTCAACACACTAGCATTTCCTAGTATGAAGAGGAAAGACGTTGTAGATGAAAAACAACCATGGGTATATCTAAACTGCGGCCATGTACATGGCTATCATAACTGGGGAAACAAAGAAGAACGTGATGGAAAAGATCGTGAATGTCCTATGTGTAGGTCTGTTGGTCCCTATGTTCCTCTGTGGCTTGGATGTGAAGCTGGATTTTATGTGGACGCCGGCCCTCCAACCCATGCGTTTAGCCCGTGTGGGCATGTGTGTTCAGAAAAGACAACTGCCTATTGGTCCCAGATCCCACTTCCTCATGGTACTCATACTTTTCATGCAGCCTGTCCCTTTTGTGCACATCAGTTGGCTGGTGAACAAGGCTACATCAGACTTATTTTTCAAGGACCTCTAGACTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSPGSLEVLFQGPGSMFSPDQENHPFKAPVKYGELIVLGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTPQAAKAISNKDQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFVVTDTVPGSQSNSDTQSVQSTISRFACRIICERNPPFTARIYAAGFDSSKNIFLGEKAAKWKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIWREISVCGNVFSLRETRSAQQRGKMVEIETNQLQDGSLIDLCGATLLWRTAEGLSHTPTVKHLEALRQEINAARPQCPVGFNTLAFPSMKRKDVVDEKQPWVYLNCGHVHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCEAGFYVDAGPPTHAFSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIFQGPLD*
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSPGSLEVLFQGPGSMFSPDQENHPFKAPVKYGELIVLGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTPQAAKAISNKDQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFVVTDTVPGSQSNSDTQSVQSTISRFACRIICERNPPFTARIYAAGFDSSKNIFLGEKAAKWKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIWREISVCGNVFSLRETRSAQQRGKMVEIETNQLQDGSLIDLCGATLLWRTAEGLSHTPTVKHLEALRQEINAARPQCPVGFNTLAFPSMKRKDVVDEKQPWVYLNCGHVHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCEAGFYVDAGPPTHAFSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIFQGPLD*
Antibiotic
Kan
Comments
silent mutation at K271 to KO internal HindIII site
100042 R 1000 1001 1047 19007
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