This reagent and more can be found at our sister site MRC PPU Reagents & Services
Request Now
Synonyms
HECTH2
Plasmid
pGEX6P.Halo.TEV UBE3C A636-S1083(end) K868R K907R
Parent Plasmid
pGEX6P1 Halo TEV
Expressed
GST.Halo-TEV-UBE3C A636-S1083(end) K868R K907R
DU Number
DU45875
Genbank
NM_014671.2 GI:187960099 with silent change a1686g
Tag
GST
HALO
Species
Human
Sequence of Insert
View Sequence
GAATTCGCAGATAAGGTCACTCAGCTCTATGTGCCAGCATCCAGACATGTGTGGAGGTTCCGGCGGATGGGGAGGATAGGCCCGCTGCAGTCCACCCTGGACGTGGGTTTGGAGTCCCCGCCGCTGTCTGTGTCTGAGGAAAGACAGCTTGCTGTCCTGACAGAGTTGCCTTTTGTGGTTCCATTTGAGGAACGAGTAAAGATCTTTCAGAGGTTGATTTATGCAGATAAGCAAGAAGTTCAAGGAGATGGTCCATTTCTGGATGGAATTAATGTCACAATAAGAAGAAATTACATTTATGAAGATGCTTATGACAAACTTTCTCCAGAAAATGAGCCTGATTTGAAAAAGCGGATCCGTGTGCACTTGCTCAATGCCCATGGCCTGGATGAAGCTGGCATTGATGGTGGTGGTATTTTCAGAGAGTTTTTAAATGAACTACTGAAGTCAGGATTTAACCCCAACCAGGGGTTCTTTAAGACTACTAATGAAGGGCTTCTGTACCCCAACCCGGCTGCTCAGATGCTTGTGGGAGATTCTTTTGCCAGACATTACTACTTCCTAGGCAGAATGCTTGGAAAGGCTCTCTATGAGAACATGCTGGTGGAGCTGCCCTTTGCAGGCTTCTTTCTTTCCAAGTTGCTTGGAACCAGTGCCGACGTGGACATTCACCACCTCGCCTCCCTAGACCCTGAGGTGTATAGAAATTTGCTCTTTCTGAAGAGCTACGAAGACGATGTGGAGGAGCTTGGGCTGAACTTCACTGTGGTGAACAATGACCTGGGAGAGGCGCAGGTAGTTGAACTAAAATTCGGTGGGCGGGACATCCCTGTCACCAGCGCCAACCGGATTGCGTACATCCACTTGGTGGCAGACTACAGGCTGAACAGGCAGATCCGCCAGCACTGCCTGGCTTTCCGCCAGGGCCTTGCCAATGTCGTCAGCCTCGAGTGGCTCCGAATGTTTGATCAGCAAGAAATTCAGGTATTAATTTCTGGTGCACAAGTTCCCATAAGCCTAGAGGACCTAAAATCCTTTACAAACTATTCAGGAGGCTATTCTGCAGACCATCCTGTTATTAAGGTCTTCTGGAGAGTTGTGGAAGGGTTCACTGATGAAGAAAAGCGCAAACTGCTGAAGTTTGTAACAAGCTGCTCTCGACCCCCTCTCTTGGGGTTTAAGGAGTTGTATCCCGCATTTTGTATTCACAACGGAGGCTCCGACCTTGAGCGGCTCCCCACAGCCAGCACCTGCATGAACCTGCTGAAGCTCCCCGAGTTCTATGACGAGACACTTTTGCGAAGTAAACTTCTCTATGCGATTGAATGTGCCGCTGGCTTTGAGCTGAGCTGAgcggccgc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGENLYFQGGSPEFADKVTQLYVP
ASRHVWRFRRMGRIGPLQSTLDVGLESPPLSVSEERQLAVLTELPFVVPF
EERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENE
PDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTT
NEGLLYPNPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPFAGFFLS
KLLGTSADVDIHHLASLDPEVYRNLLFLKSYEDDVEELGLNFTVVNNDLG
EAQVVELKFGGRDIPVTSANRIAYIHLVADYRLNRQIRQHCLAFRQGLAN
VVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSADHPVIKV
FWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERL
PTASTCMNLLKLPEFYDETLLRSKLLYAIECAAGFELS*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGENLYFQGGSPEFADKVTQLYVP
ASRHVWRFRRMGRIGPLQSTLDVGLESPPLSVSEERQLAVLTELPFVVPF
EERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENE
PDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTT
NEGLLYPNPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPFAGFFLS
KLLGTSADVDIHHLASLDPEVYRNLLFLKSYEDDVEELGLNFTVVNNDLG
EAQVVELKFGGRDIPVTSANRIAYIHLVADYRLNRQIRQHCLAFRQGLAN
VVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSADHPVIKV
FWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERL
PTASTCMNLLKLPEFYDETLLRSKLLYAIECAAGFELS*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Parental: DU24005
100042 R 1000 1001 1047 19007
Download datasheet