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Synonyms
E2-230K
Plasmid
pGEX6P UBE20 Q57-E1290
Parent Plasmid
pGEX6P1
Expressed
GST UBE20 Q57-E1290
DU Number
DU45530
Genbank
NM_022066.3 GI:192449448 with silent change g6a (to kill BamH1) and coding change g3619a (G1207S) - common variant
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCCAGCGCCTGCTGTTTTCTCACGACCTGGTGTCGGGCCGTTACCGTGGCTCCGTGCACTTCGGGCTGGTGCGCCTCATCCACGGCGAGGACTCGGACTCGGAGGGCGAGGAGGAGGGCCGCGGGAGCTCGGGGTGCTCCGAGGCCGGGGGCGCGGGCCACGAGGAGGGCCGGGCCAGCCCCCTGCGCCGCGGCTACGTGCGCGTCCAGTGGTACCCGGAGGGCGTCAAGCAGCATGTGAAGGAGACCAAGCTGAAACTAGAGGACCGTTCTGTGGTGCCCCGAGATGTGGTCCGGCACATGCGATCCACCGACAGTCAGTGTGGCACGGTGATCGACGTCAACATCGACTGTGCCGTCAAGCTCATCGGCACCAACTGCATCATCTATCCCGTCAACAGCAAGGACCTGCAGCACATCTGGCCCTTCATGTATGGGGACTACATTGCCTATGACTGCTGGCTGGGGAAGGTCTACGACTTGAAGAACCAGATCATCCTGAAGCTATCCAACGGCGCCAGGTGCTCCATGAACACGGAAGATGGCGCCAAGCTCTACGACGTCTGCCCGCACGTCAGCGACTCGGGTCTCTTCTTCGATGATTCCTATGGCTTCTACCCAGGCCAGGTGCTCATTGGCCCTGCCAAGATCTTCTCCAGCGTCCAGTGGCTGTCAGGTGTCAAGCCCGTGCTCAGCACCAAGAGCAAGTTCCGAGTGGTGGTGGAAGAGGTGCAGGTTGTAGAGTTGAAAGTTACATGGATTACCAAGAGTTTCTGTCCAGGGGGCACGGACAGCGTCAGCCCCCCACCCTCTGTCATCACCCAGGAAAACCTAGGCAGGGTGAAGCGTCTCGGATGCTTTGACCATGCTCAGCGGCAGCTTGGGGAGCGCTGTCTGTATGTCTTCCCAGCCAAGGTAGAGCCAGCCAAGATTGCCTGGGAATGTCCAGAAAAAAACTGCGCCCAGGGGGAGGGCTCTATGGCCAAGAAGGTGAAGCGCCTGTTGAAGAAGCAGGTTGTGCGGATCATGTCATGCTCCCCAGACACCCAGTGTTCCCGGGACCATTCCATGGAAGACCCAGACAAGAAGGGGGAATCCAAAACCAAGAGCGAAGCGGAGTCTGCCAGCCCTGAGGAGACGCCCGATGGCTCTGCCAGTCCAGTGGAGATGCAGGACGAGGGTGCAGAGGAGCCCCACGAGGCAGGAGAGCAGCTGCCCCCATTCCTGCTAAAAGAAGGCAGAGATGACAGGCTGCACTCGGCAGAGCAGGACGCAGATGATGAGGCTGCTGATGACACGGACGACACCAGTTCGGTGACCTCCTCTGCCAGCTCCACCACTTCCTCCCAGAGCGGCAGCGGCACGAGTCGCAAAAAGAGCATCCCCTTGTCCATCAAGAACTTAAAGCGCAAACACAAGAGGAAGAAGAATAAAATCACTCGAGACTTCAAGCCAGGGGACAGGGTGGCAGTGGAGGTGGTGACCACGATGACCTCAGCCGACGTGATGTGGCAGGATGGCTCCGTGGAATGCAACATCCGCTCCAACGACCTCTTCCCTGTGCACCACCTGGACAACAACGAGTTCTGCCCTGGAGACTTCGTGGTAGATAAGCGAGTCCAGAGCTGTCCAGACCCTGCTGTCTACGGTGTGGTACAGTCTGGGGACCACATCGGCCGTACCTGCATGGTGAAGTGGTTCAAGCTGAGGCCGAGTGGGGACGACGTGGAGCTGATTGGAGAAGAGGAAGATGTGAGTGTTTACGACATTGCTGACCACCCTGACTTTAGGTTCCGTACAACTGACATCGTCATCCGCATCGGCAATACTGAGGATGGGGCTCCTCACAAGGAGGATGAGCCATCGGTGGGCCAGGTGGCCCGTGTGGACGTCAGCAGCAAGGTGGAGGTGGTGTGGGCTGACAACTCAAAGACCATCATCCTGCCCCAGCACTTGTACAACATAGAGTCTGAGATTGAGGAGTCAGACTACGATTCGGTAGAAGGCAGCACCAGCGGGGCATCCTCGGATGAATGGGAAGATGATAGTGACAGCTGGGAGACGGACAATGGGCTGGTGGAGGACGAGCACCCCAAGATAGAGGAGCCCCCCATCCCACCCCTGGAGCAGCCGGTGGCCCCTGAGGACAAGGGAGTGGTGATCAGTGAAGAGGCAGCCACAGCTGCCGTCCAGGGGGCTGTGGCCATGGCTGCCCCCATGGCCGGGCTGATGGAGAAGGCTGGCAAGGACGGGCCACCCAAGAGCTTCCGGGAGTTGAAAGAGGCCATCAAGATCCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGCTGACGGGCTCGCCCACCTCTCCGACTGTGGAGCCTGAGAAGCCAACTCGGGAGAAGAAGTTTCTGGATGACATCAAGAAGCTACAGGAAAACCTCAAGAAGACCCTGGACAATGTGGCCATTGTAGAGGAGGAGAAGATGGAAGCAGTGCCCGACGTAGAGCGCAAGGAGGACAAGCCCGAGGGGCAGTCACCTGTGAAGGCTGAGTGGCCCAGCGAAACCCCGGTGCTGTGCCAGCAGTGTGGCGGCAAGCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTCTCCGTACTGGAGTTTGCACCCTCAAATCATTCTTTTAAGAAAATTGAGTTCCAGCCTCCAGAAGCCAAGAAGTTCTTCAGCACAGTGCGGAAGGAGATGGCGCTGCTGGCTACCTCACTGCCTGAGGGCATCATGGTCAAGACTTTTGAAGATAGAATGGACCTCTTCTCAGCTCTCATCAAGGGCCCCACTCGAACCCCCTACGAGGATGGCCTCTACTTGTTTGACATCCAGCTCCCCAACATCTACCCAGCCGTGCCCCCCCACTTCTGCTACCTCTCCCAATGCAGTGGCCGCCTGAACCCCAACCTGTATGACAATGGGAAGGTGTGTGTCAGCCTCCTGGGCACCTGGATTGGAAAGGGGACAGAGAGGTGGACAAGCAAGTCCAGCCTTCTCCAGGTGCTCATCTCCATCCAAGGTCTGATCCTGGTAAATGAACCATACTACAACGAAGCCGGCTTCGACAGTGACCGAGGCCTGCAGGAAGGCTATGAAAACAGTCGCTGTTACAATGAGATGGCGCTGATCCGCGTGGTGCAGTCCATGACCCAGCTGGTGCGGCGGCCCCCCGAGGTCTTTGAGCAGGAGATCAGGCAACACTTTAGCACTGGTGGCTGGCGGCTGGTGAACCGTATCGAGTCCTGGCTGGAAACCCATGCCCTGCTGGAGAAGGCCCAGGCACTGCCCAACGGGGTGCCCAAGGCCAGCAGCTCGCCAGAGCCCCCAGCTGTAGCCGAGCTGTCAGACTCCGGCCAACAAGAACCTGAGGATGGAGGGCCAGCCCCAGGAGAGGCCTCCCAGGGCTCAGACTCAGAGGGCGGTGCCCAGAGCCTGGCCTCAGCTAGCAGGGACCACACAGACCAGACTTCGGAGACCGCACCAGACGCATCGGTGCCACCCAGTGTGAAACCAAAGAAGCGGAGAAAGAGCTACCGGAGCTTCTTACCTGAGAAGAGTGGCTACCCTGACATCGGCTTCCCCCTCTTCCCACTTTCCAAGGGTTTCATCAAGAGCATCCGGGGTGTCCTGACGCAGTTCCGGGCTGCCCTGCTAGAGGCAGGCATGCCGGAGTGCACAGAGTAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQRLLFSHDLVSGRYRGSVH
FGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQ
WYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAV
KLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLS
NGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSS
VQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPP
SVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNC
AQGEGSMAKKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKS
EAESASPEETPDGSASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHS
AEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKR
KHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFP
VHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLR
PSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDE
PSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGS
TSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVV
ISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLK
NMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVE
EEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAK
GEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMV
KTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQC
SGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVN
EPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQ
EIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVA
ELSDSGQQEPEDGGPAPGEASQGSDSEGGAQSLASASRDHTDQTSETAPD
ASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQ
FRAALLEAGMPECTE*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQRLLFSHDLVSGRYRGSVH
FGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQ
WYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAV
KLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLS
NGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSS
VQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPP
SVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNC
AQGEGSMAKKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKS
EAESASPEETPDGSASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHS
AEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKR
KHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFP
VHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLR
PSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDE
PSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGS
TSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVV
ISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLK
NMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVE
EEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAK
GEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMV
KTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQC
SGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVN
EPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQ
EIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVA
ELSDSGQQEPEDGGPAPGEASQGSDSEGGAQSLASASRDHTDQTSETAPD
ASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQ
FRAALLEAGMPECTE*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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