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Synonyms
E2-230K
Plasmid
pcDNA5 FRT/TO GFP UBE20
Parent Plasmid
pcDNA5D FRT/TO GFP
Expressed
GFP UBE20
DU Number
DU45507
Genbank
NM_022066.3 GI:192449448 with silent change g6a (to kill BamH1) and coding change g3619a (G1207S) - common variant
Tag
GFP
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGCAGATCCCGCAGCCCCCACGCCCGCAGCTCCCGCTCCAGCCCAGGCCCCGGCTCCAGCCCCGGAGGCAGTCCCGGCCCCAGCCGCAGCCCCCGTCCCGGCGCCGGCGCCCGCCTCGGACTCGGCCTCCGGGCCGTCCTCGGACTCCGGCCCAGAAGCCGGCTCGCAGCGCCTGCTGTTTTCTCACGACCTGGTGTCGGGCCGTTACCGTGGCTCCGTGCACTTCGGGCTGGTGCGCCTCATCCACGGCGAGGACTCGGACTCGGAGGGCGAGGAGGAGGGCCGCGGGAGCTCGGGGTGCTCCGAGGCCGGGGGCGCGGGCCACGAGGAGGGCCGGGCCAGCCCCCTGCGCCGCGGCTACGTGCGCGTCCAGTGGTACCCGGAGGGCGTCAAGCAGCATGTGAAGGAGACCAAGCTGAAACTAGAGGACCGTTCTGTGGTGCCCCGAGATGTGGTCCGGCACATGCGATCCACCGACAGTCAGTGTGGCACGGTGATCGACGTCAACATCGACTGTGCCGTCAAGCTCATCGGCACCAACTGCATCATCTATCCCGTCAACAGCAAGGACCTGCAGCACATCTGGCCCTTCATGTATGGGGACTACATTGCCTATGACTGCTGGCTGGGGAAGGTCTACGACTTGAAGAACCAGATCATCCTGAAGCTATCCAACGGCGCCAGGTGCTCCATGAACACGGAAGATGGCGCCAAGCTCTACGACGTCTGCCCGCACGTCAGCGACTCGGGTCTCTTCTTCGATGATTCCTATGGCTTCTACCCAGGCCAGGTGCTCATTGGCCCTGCCAAGATCTTCTCCAGCGTCCAGTGGCTGTCAGGTGTCAAGCCCGTGCTCAGCACCAAGAGCAAGTTCCGAGTGGTGGTGGAAGAGGTGCAGGTTGTAGAGTTGAAAGTTACATGGATTACCAAGAGTTTCTGTCCAGGGGGCACGGACAGCGTCAGCCCCCCACCCTCTGTCATCACCCAGGAAAACCTAGGCAGGGTGAAGCGTCTCGGATGCTTTGACCATGCTCAGCGGCAGCTTGGGGAGCGCTGTCTGTATGTCTTCCCAGCCAAGGTAGAGCCAGCCAAGATTGCCTGGGAATGTCCAGAAAAAAACTGCGCCCAGGGGGAGGGCTCTATGGCCAAGAAGGTGAAGCGCCTGTTGAAGAAGCAGGTTGTGCGGATCATGTCATGCTCCCCAGACACCCAGTGTTCCCGGGACCATTCCATGGAAGACCCAGACAAGAAGGGGGAATCCAAAACCAAGAGCGAAGCGGAGTCTGCCAGCCCTGAGGAGACGCCCGATGGCTCTGCCAGTCCAGTGGAGATGCAGGACGAGGGTGCAGAGGAGCCCCACGAGGCAGGAGAGCAGCTGCCCCCATTCCTGCTAAAAGAAGGCAGAGATGACAGGCTGCACTCGGCAGAGCAGGACGCAGATGATGAGGCTGCTGATGACACGGACGACACCAGTTCGGTGACCTCCTCTGCCAGCTCCACCACTTCCTCCCAGAGCGGCAGCGGCACGAGTCGCAAAAAGAGCATCCCCTTGTCCATCAAGAACTTAAAGCGCAAACACAAGAGGAAGAAGAATAAAATCACTCGAGACTTCAAGCCAGGGGACAGGGTGGCAGTGGAGGTGGTGACCACGATGACCTCAGCCGACGTGATGTGGCAGGATGGCTCCGTGGAATGCAACATCCGCTCCAACGACCTCTTCCCTGTGCACCACCTGGACAACAACGAGTTCTGCCCTGGAGACTTCGTGGTAGATAAGCGAGTCCAGAGCTGTCCAGACCCTGCTGTCTACGGTGTGGTACAGTCTGGGGACCACATCGGCCGTACCTGCATGGTGAAGTGGTTCAAGCTGAGGCCGAGTGGGGACGACGTGGAGCTGATTGGAGAAGAGGAAGATGTGAGTGTTTACGACATTGCTGACCACCCTGACTTTAGGTTCCGTACAACTGACATCGTCATCCGCATCGGCAATACTGAGGATGGGGCTCCTCACAAGGAGGATGAGCCATCGGTGGGCCAGGTGGCCCGTGTGGACGTCAGCAGCAAGGTGGAGGTGGTGTGGGCTGACAACTCAAAGACCATCATCCTGCCCCAGCACTTGTACAACATAGAGTCTGAGATTGAGGAGTCAGACTACGATTCGGTAGAAGGCAGCACCAGCGGGGCATCCTCGGATGAATGGGAAGATGATAGTGACAGCTGGGAGACGGACAATGGGCTGGTGGAGGACGAGCACCCCAAGATAGAGGAGCCCCCCATCCCACCCCTGGAGCAGCCGGTGGCCCCTGAGGACAAGGGAGTGGTGATCAGTGAAGAGGCAGCCACAGCTGCCGTCCAGGGGGCTGTGGCCATGGCTGCCCCCATGGCCGGGCTGATGGAGAAGGCTGGCAAGGACGGGCCACCCAAGAGCTTCCGGGAGTTGAAAGAGGCCATCAAGATCCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGCTGACGGGCTCGCCCACCTCTCCGACTGTGGAGCCTGAGAAGCCAACTCGGGAGAAGAAGTTTCTGGATGACATCAAGAAGCTACAGGAAAACCTCAAGAAGACCCTGGACAATGTGGCCATTGTAGAGGAGGAGAAGATGGAAGCAGTGCCCGACGTAGAGCGCAAGGAGGACAAGCCCGAGGGGCAGTCACCTGTGAAGGCTGAGTGGCCCAGCGAAACCCCGGTGCTGTGCCAGCAGTGTGGCGGCAAGCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTCTCCGTACTGGAGTTTGCACCCTCAAATCATTCTTTTAAGAAAATTGAGTTCCAGCCTCCAGAAGCCAAGAAGTTCTTCAGCACAGTGCGGAAGGAGATGGCGCTGCTGGCTACCTCACTGCCTGAGGGCATCATGGTCAAGACTTTTGAAGATAGAATGGACCTCTTCTCAGCTCTCATCAAGGGCCCCACTCGAACCCCCTACGAGGATGGCCTCTACTTGTTTGACATCCAGCTCCCCAACATCTACCCAGCCGTGCCCCCCCACTTCTGCTACCTCTCCCAATGCAGTGGCCGCCTGAACCCCAACCTGTATGACAATGGGAAGGTGTGTGTCAGCCTCCTGGGCACCTGGATTGGAAAGGGGACAGAGAGGTGGACAAGCAAGTCCAGCCTTCTCCAGGTGCTCATCTCCATCCAAGGTCTGATCCTGGTAAATGAACCATACTACAACGAAGCCGGCTTCGACAGTGACCGAGGCCTGCAGGAAGGCTATGAAAACAGTCGCTGTTACAATGAGATGGCGCTGATCCGCGTGGTGCAGTCCATGACCCAGCTGGTGCGGCGGCCCCCCGAGGTCTTTGAGCAGGAGATCAGGCAACACTTTAGCACTGGTGGCTGGCGGCTGGTGAACCGTATCGAGTCCTGGCTGGAAACCCATGCCCTGCTGGAGAAGGCCCAGGCACTGCCCAACGGGGTGCCCAAGGCCAGCAGCTCGCCAGAGCCCCCAGCTGTAGCCGAGCTGTCAGACTCCGGCCAACAAGAACCTGAGGATGGAGGGCCAGCCCCAGGAGAGGCCTCCCAGGGCTCAGACTCAGAGGGCGGTGCCCAGAGCCTGGCCTCAGCTAGCAGGGACCACACAGACCAGACTTCGGAGACCGCACCAGACGCATCGGTGCCACCCAGTGTGAAACCAAAGAAGCGGAGAAAGAGCTACCGGAGCTTCTTACCTGAGAAGAGTGGCTACCCTGACATCGGCTTCCCCCTCTTCCCACTTTCCAAGGGTTTCATCAAGAGCATCCGGGGTGTCCTGACGCAGTTCCGGGCTGCCCTGCTAGAGGCAGGCATGCCGGAGTGCACAGAGGACAAGTAGCGGCCGC
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMADPAA
PTPAAPAPAQAPAPAPEAVPAPAAAPVPAPAPASDSASGPSSDSGPEAGS
QRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGG
AGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHM
RSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAY
DCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDD
SYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKV
TWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYV
FPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTQC
SRDHSMEDPDKKGESKTKSEAESASPEETPDGSASPVEMQDEGAEEPHEA
GEQLPPFLLKEGRDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQS
GSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSAD
VMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGV
VQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTT
DIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQH
LYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIE
EPPIPPLEQPVAPEDKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDG
PPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDD
IKKLQENLKKTLDNVAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSET
PVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFS
TVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDI
QLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWT
SKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALI
RVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQ
ALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQ
SLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGF
PLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK*
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMADPAA
PTPAAPAPAQAPAPAPEAVPAPAAAPVPAPAPASDSASGPSSDSGPEAGS
QRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGG
AGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHM
RSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAY
DCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDD
SYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKV
TWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYV
FPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTQC
SRDHSMEDPDKKGESKTKSEAESASPEETPDGSASPVEMQDEGAEEPHEA
GEQLPPFLLKEGRDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQS
GSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSAD
VMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGV
VQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTT
DIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQH
LYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIE
EPPIPPLEQPVAPEDKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDG
PPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDD
IKKLQENLKKTLDNVAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSET
PVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFS
TVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDI
QLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWT
SKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALI
RVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQ
ALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQ
SLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGF
PLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK*
Vector Type
Mammalian
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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