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Plasmid
pFBDM-Rbx1, KLHL3 R528H, DAC_TEV-Cullin3
Parent Plasmid
pFBDM
Expressed
DAC-Cullin3 (insect optimised)
DU Number
DU23401
Tag
DAC
Species
Human
Sequence of Insert
View Sequence
GGATCCGAAAACCTGTATTTTCAGATGTCTAACCTGTCTAAGGGCACTGGCAGCCGTAAGGATACCAAGATGAGAATCAGAGCCTTCCCGATGACTATGGACGAAAAGTACGTGAACTCCATCTGGGACCTGCTCAAGAACGCTATCCAGGAGATCCAAAGGAAGAACAACTCCGGCTTGTCTTTCGAGGAACTGTACAGAAACGCCTACACTATGGTGCTGCACAAGCACGGCGAGAAGCTCTACACTGGCTTGCGTGAAGTGGTCACAGAGCACCTCATCAACAAGGTTCGCGAAGATGTGCTCAACTCTTTGAACAACAACTTCCTGCAGACTCTCAACCAGGCTTGGAACGACCACCAGACAGCTATGGTCATGATCAGGGATATCCTGATGTACATGGACAGAGTCTACGTTCAGCAAAACAACGTGGAGAACGTCTACAACCTCGGATTGATCATCTTCCGTGACCAGGTTGTGCGCTACGGTTGCATCCGTGATCACCTCCGCCAAACCTTGCTGGACATGATCGCTCGTGAACGCAAGGGAGAGGTCGTTGATAGGGGTGCTATCAGAAACGCCTGTCAGATGCTGATGATCCTGGGTCTCGAAGGCAGGAGCGTCTACGAGGAAGACTTCGAGGCTCCCTTCCTCGAAATGTCGGCCGAGTTCTTCCAGATGGAATCCCAAAAGTTCTTGGCTGAGAACAGCGCCTCAGTCTACATCAAGAAGGTTGAAGCCCGTATCAACGAGGAAATCGAGCGCGTTATGCACTGCCTGGACAAGTCAACCGAGGAACCTATCGTCAAGGTGGTCGAGCGCGAACTCATCTCCAAGCACATGAAGACCATCGTCGAGATGGAAAACTCTGGTCTGGTTCACATGCTCAAGAACGGCAAGACTGAGGACCTGGGATGTATGTACAAGCTCTTCTCCAGGGTGCCCAACGGCCTGAAGACTATGTGCGAATGTATGTCCTCTTACCTCAGAGAGCAGGGAAAGGCTTTGGTTTCTGAGGAAGGCGAGGGAAAGAACCCTGTGGACTACATCCAAGGCCTCTTGGATTTGAAGTCTAGGTTCGACCGTTTCCTGCTGGAGTCCTTCAACAACGATAGGCTGTTCAAGCAGACCATCGCCGGAGACTTCGAGTACTTCTTGAACCTGAACTCCCGTTCCCCTGAATACCTCAGCTTGTTCATCGACGATAAGCTGAAGAAGGGAGTGAAGGGTCTCACCGAGCAAGAAGTCGAGACTATCTTGGATAAGGCTATGGTCCTGTTCCGTTTCATGCAGGAAAAGGACGTTTTCGAGCGCTACTACAAGCAACACCTGGCCCGTCGCTTGCTGACTAACAAGTCTGTGAGCGACGATAGCGAAAAGAACATGATCTCAAAGTTGAAGACCGAGTGCGGTTGTCAGTTCACTTCTAAGCTGGAAGGCATGTTCCGTGATATGTCAATCTCGAACACCACTATGGACGAGTTCCGCCAGCACCTGCAAGCTACCGGTGTGTCACTCGGTGGCGTGGACCTGACTGTGCGTGTCCTGACAACCGGCTACTGGCCCACTCAGTCGGCTACACCTAAGTGCAACATCCCCCCTGCTCCACGTCACGCCTTCGAAATCTTCAGGAGATTCTACTTGGCCAAGCACAGCGGACGCCAGCTGACACTCCAACACCACATGGGTTCAGCTGATCTGAACGCCACCTTCTACGGCCCGGTGAAGAAGGAAGACGGCTCCGAGGTTGGAGTGGGAGGTGCTCAGGTGACCGGTTCAAACACTCGCAAGCACATCCTGCAGGTCTCGACATTCCAAATGACCATCTTGATGCTGTTCAACAACAGGGAGAAGTACACATTCGAGGAAATCCAGCAAGAAACCGACATCCCAGAACGTGAGCTGGTGCGTGCTCTGCAGTCGCTGGCTTGCGGAAAGCCAACTCAACGCGTCTTGACAAAGGAGCCGAAGTCCAAGGAAATCGAGAACGGTCACATCTTCACAGTGAACGATCAGTTCACCTCTAAGCTGCACCGTGTTAAGATCCAGACTGTGGCTGCCAAGCAAGGCGAGAGCGACCCCGAAAGGAAGGAGACAAGACAGAAGGTGGACGATGACCGCAAGCACGAAATCGAGGCTGCCATCGTCAGGATCATGAAGAGCAGAAAGAAGATGCAACACAACGTCCTCGTTGCTGAGGTCACCCAGCAACTCAAGGCCAGGTTCTTGCCATCACCGGTTGTGATCAAGAAGAGAATCGAGGGTCTGATCGAGAGAGAATACTTGGCTAGGACTCCAGAGGACAGAAAGGTTTACACTTACGTTGCTTAAaagctt
Amino Acid Sequence
View Sequence
MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPGSENLYFQMSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA*KL
Vector Type
Baculovirus
Antibiotic
Amp
Comments
blunt TEV
100042 R 1000 1001 1047 19007
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