UBE2W

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Plasmid
pET28a-UBE2W isoform 1
Parent Plasmid
pET28a original
Expressed
6His-UBE2W
DU Number
DU20190
Genbank
NM_001001481.1
Tag
6His
Species
Human
Sequence of Insert
GGATCCATGGCGTCAATGCAGACCACAGGAAGGAGGGTGGAAGTATGGTT
TCCAAAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCACCTC
CTGGAATGACCTTAAATGAGAAGAGTGTTCAAAATTCAATTACACAGTGG
ATTGTAGACATGGAAGGTGCACCAGGTACCTTATATGAAGGGGAAAAATT
TCAACTTCTATTTAAATTTAGTAGTCGATATCCTTTTGACTCTCCTCAGG
TCATGTTTACTGGTGAAAATATTCCTGTTCATCCTCATGTTTATAGCAAT
GGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCCCCAGCGCTCTC
AGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAGG
AAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAG
AATCCAAAGAAAACAAAATGGTGGTATCATGATGATACTTGTTGAGCGGC
CGC
Amino Acid Sequence
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMASMQTTGRRVEVWFP
KRLQKELLALQNDPPPGMTLNEKSVQNSITQWIVDMEGAPGTLYEGEKFQ
LLFKFSSRYPFDSPQVMFTGENIPVHPHVYSNGHICLSILTEDWSPALSV
QSVCLSIISMLSSCKEKRRPPDNSFYVRTCNKNPKKTKWWYHDDTC*





Vector Type
Bacterial
Antibiotic
Kan
Comments
Bacterial expression construct to express UBE2W with N-terminal 6HIS tag (contains Thrombin cleavage site)

Estimated MW of expressed protein : 22205 Daltons
100042 R 1000 1001 1047 19007
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